We narrowed to 9,391 results for: Coli
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Plasmid#119853Purposeprecusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427CDepositorInsertprecursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
UseTagsnoneExpressionBacterialMutationCysteine 169 to Serine, Cysteine 179 to Serine, C…PromoterT7Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCarCBADE
Plasmid#117757PurposeCodon optimized CarCBADE enzymes from Pectobacterium carotovorum in pBbaA5k backboneDepositorInsertCodon optimised CarCBA, Protein CarD, Ferredoxin CarE
UseTagsExpressionBacterialMutationPromoterPLac(UV5)Available sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbH-C-A2-PCP S1524A
Plasmid#114158PurposeExpresses ClbH-C-A2-PCP S1524ADepositorInsertclbH
UseTagsS-Tag, Thrombin siteExpressionBacterialMutationOnly contains the C-A2-PCP domains of ClbH with S…PromoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJB03
Plasmid#111961PurposeExpresses MBP-GFP-DHFR; construct generated for pull-down and localization experimentsDepositorInsertsmEGFP
eDHFR
UseTagsDHFRExpressionBacterialMutationeDHFR;residues 2-159 and enhanced GFP with monome…PromoterTacAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-PdhR
Plasmid#89655PurposePlasmid for titrating synthesis of PdhR with doxycyclineDepositorInsertPyruvate dehydrogenase complex repressor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-FtsB
Plasmid#107559PurposePlasmid for titrating synthesis of FtsB with doxycyclineDepositorInsertftsB
UseSynthetic BiologyTagsExpressionMutationPromoterpLtetOAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU2-D-lacZ
Plasmid#72953PurposeLevel 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - D site
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2-D-RFP
Plasmid#72957PurposeLevel 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - D site
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Vv4CL-PhCHS-MsCHI
Plasmid#73400PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsVv4CL
PhCHS
MsCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Vv4CL-CmCHS-CmCHI
Plasmid#73403PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsVv4CL
CmCHS
CmCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Pc4CL-CmCHS-CmCHI
Plasmid#73399PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsPc4CL
CmCHS
CmCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Rm_EF
Plasmid#66060PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Ampicillin plasmid [E:J23102:B:BCD2:C:E1010m:D:B0015:F]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Rm_AE
Plasmid#66065PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Rm_EF
Plasmid#66066PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E1010m:D:B0015:F]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Gm_AE
Plasmid#66057PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Gm_EF
Plasmid#66058PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Ampicillin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Rm_AE
Plasmid#66059PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_AB
Plasmid#66016PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [A:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_EB
Plasmid#66017PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [E:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_FB
Plasmid#66018PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [F:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_GB
Plasmid#66019PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [G:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
B0033m_BC
Plasmid#66021PurposeMoClo Basic Part: RBS - Weiss RBS, low strength. Modified from Bba_B0033 to adjust spacing in MC system. [B:B0033m:C]DepositorInsertRBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
B0034m_BC
Plasmid#66022PurposeMoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0034 to adjust spacing in MC system. [B:B0034m:C]DepositorInsertRBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
C0080 (araC)_CD
Plasmid#66029PurposeMoClo Basic Part: CDS - Controller protein, araC repressor/activator (activates pBAD, I13453) [C:C0080:D]DepositorInsertTranscription factor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZS1[Iq:LacI-T]
Plasmid#60756PurposeContains PIq driving expression LacI-T, the Lactose inducible chimera with the TAN DBD.DepositorInsertLacI-T
UseSynthetic BiologyTagsExpressionBacterialMutationLacI without the tetramerization domain and the T…PromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 1035
Plasmid#52999PurposepLtetO-1:RhyB, pCon:fhlA::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
fhlA
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains −309 to +96 bp relative to start codon a…PromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1033
Plasmid#52998PurposepLtetO-1:RhyB, pCon:rpoS::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
rpoS
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains −150 and +30 bp relative to start codon …PromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa02_2817
Plasmid#49654DepositorInsertECF02_2817
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef-Ptef1-CSC-Tpdc1
Plasmid#127739PurposeYeast pathway position 6. CSC transcription unit with the TEF1 promoter and PDC1 terminator.DepositorInsertcitrate synthase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfba1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef-Pzuo1-CSC-Tvma16
Plasmid#127740PurposeYeast pathway position 6. CSC transcription unit with the ZUO1 promoter and VMA16 terminator.DepositorInsertcitrate synthase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPzuo1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef-Ppfy1-CSC-Taip1
Plasmid#127741PurposeYeast pathway position 6. CSC transcription unit with the PFY1 promoter and AIP1 terminator.DepositorInsertcitrate synthase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPpfy1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
BirA in pET28a (w400-2)
Plasmid#26624DepositorInsertBirA
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOP3SU
Plasmid#112599PurposeUsed for expression of protein in E. coli as N-terminal Sumo tag fusionsDepositorTypeEmpty backboneUseTagsSumoExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpot2
Plasmid#117063PurposeFor expression of proteins with C-terminal Spot-Tag in E. coliDepositorTypeEmpty backboneUseTagsSpot-TagExpressionBacterialMutationPromoterAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP2H
Plasmid#112596PurposeUsed for expression of protein in E. coli as non-cleavable C-terminal 8His-tag fusionsDepositorTypeEmpty backboneUseTags8HisExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTar-ipgC
Plasmid#97097PurposeExpresses ipgC in the presence of E. coli sigma factor ?28DepositorInsertIpgC
UseTagsExpressionBacterialMutationPromoterpTarAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDest-557
Plasmid#161890PurposeStandard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions in E. coliDepositorTypeEmpty backboneUseTagsMBPExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF-mcherry2
Plasmid#87145PurposeConstitutive E. coli mcherry Expression, with DAS4 tagDepositorInsertmcherry
UseTagsC terminal DAS4 tagExpressionBacterialMutationPromoterConstitutive proDAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOP2S
Plasmid#112597PurposeUsed for expression of protein in E. coli as non-cleavable C-terminal Strep Tag fusionsDepositorTypeEmpty backboneUseTagsStrep tagExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAMHDAdGT8-4
Plasmid#26421DepositorInsertHSV-TK, mCMV-b-gal
UseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only