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Addgene

We narrowed to 8,862 results for: BLI;

Showing: 5351 - 5400 of 8862 results
  1. pET28-Nsp14-TEV-Strep

    Plasmid
    #154953
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli
    Depositor
    Insert
    Nsp14-TEV-Strep (ORF1ab SARS-CoV-2)
    Use
    Tags
    TEV-Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  2. pET22-Strep-TEV-Nsp10

    Plasmid
    #154958
    Purpose
    Expresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli
    Depositor
    Insert
    Strep-TEV-Nsp10 (ORF1ab SARS-CoV-2)
    Use
    Tags
    Strep-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    July 21, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. DYSF-Exon42-del

    Plasmid
    #60194
    Purpose
    Plasmid contains human dysferlin with exon 42 deleted. Deletion maintains the open reading frame.
    Depositor
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 42 has been deleted
    Promoter
    Available since
    Oct. 23, 2014
    Availability
    Academic Institutions and Nonprofits only
  4. DYSF-Exon41-del

    Plasmid
    #60193
    Purpose
    Plasmid contains human dysferlin with exon 41 deleted. Deletion maintains the open reading frame.
    Depositor
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 41 has been deleted
    Promoter
    Available since
    Oct. 23, 2014
    Availability
    Academic Institutions and Nonprofits only
  5. Dox human Citron shRNA 1 mKate

    Plasmid
    #155293
    Purpose
    Lentivirus for doxycycline inducible expression of nontargeting shRNA, mKate marker, based on Addgene 11652
    Depositor
    Insert
    Citron (CIT Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. Mouse 5' Npm1 AID GFP PuroR

    Plasmid
    #127899
    Purpose
    HDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Npm1 Gene
    Depositor
    Insert
    Npm1 (Npm1 Mouse, Mustard Weed)
    Use
    Donor plasmid
    Tags
    GFP, AID, PuroR
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. TGM1

    Plasmid
    #39068
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    TGM1 (TGM1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only
  8. GMPR

    Plasmid
    #39147
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    GMPR (GMPR Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  9. pIC145

    Plasmid
    #63068
    Purpose
    Retroviral expression of EGFP-TEV-S-tag CENP-O for protein localization and affinity purification (LAP)
    Depositor
    Insert
    CENP-O, IMAGE clone 3942844 (CENPO Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
  10. rFIXX-bp chain B (IRESpuro)

    Plasmid
    #82451
    Purpose
    Chain B of the habu snake venom FIX/FX binding protein with C-terminal biotin-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring puromycin resistance.
    Depositor
    Insert
    rFIXX-bp chain B
    Use
    Tags
    Biotin
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7 RNA polymerase promoter
    Available since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
  11. pHH0103 KIBRA WW domain #2

    Plasmid
    #104230
    Purpose
    Bacterial expression of WW domain #2 from KIBRA
    Insert
    KIBRA WW domain #2 (WWC1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  12. 4RRE

    Plasmid
    #64662
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    ITCH (ITCH Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    Available since
    May 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
  13. AMPH

    Plasmid
    #39093
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    AMPH (AMPH Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  14. HOXC5 (human) HIS-tag pET

    Plasmid
    #8563
    Depositor
    Insert
    HOXC5 (HOXC5 Human)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    Starts at Amino Acid #2 (Ser); makes protein by W…
    Promoter
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only
  15. DYSF-Exon17-del

    Plasmid
    #60182
    Purpose
    Plasmid contains human dysferlin with exon 17 deleted. Deletion maintains the open reading frame.
    Depositor
    Insert
    Dysferlin (DYSF Human)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Exon 17 has been deleted
    Promoter
    Available since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
  16. pUASP Flag Rab32 CA

    Plasmid
    #44834
    Depositor
    Insert
    Rab32 (Rab32 Fly)
    Use
    Drosophila
    Tags
    Flag
    Expression
    Insect
    Mutation
    Q79L (constitutive active)
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  17. CA6

    Plasmid
    #38992
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    CA6 (CA6 Human)
    Use
    Tags
    Tev His Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only
  18. RGS14

    Plasmid
    #39139
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    RGS14 (RGS14 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  19. pCRII-Topo Cdh10 in situ probe

    Plasmid
    #45638
    Depositor
    Insert
    Cdh10 in situ probe (Cdh10 Mouse)
    Use
    In situ
    Tags
    Expression
    Mutation
    fragment contains nt 2628-3061 of Cdh10 (GenBank …
    Promoter
    Available since
    June 3, 2013
    Availability
    Academic Institutions and Nonprofits only
  20. pHH0103 YAP1 WW domain #2

    Plasmid
    #104228
    Purpose
    Bacterial expression of WW domain #2 from YAP1
    Insert
    YAP1 WW domain #2 (YAP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  21. pHH0103 YAP1 WW domain #1

    Plasmid
    #104227
    Purpose
    Bacterial expression of WW domain #1 from YAP1
    Insert
    YAP1 WW domain #1 (YAP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  22. CCNT2

    Plasmid
    #38960
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    CCNT2 (CCNT2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only
  23. pJK383

    Plasmid
    #72474
    Purpose
    Produces Thermoplasma acidophilum citrate synthase with Asp317>Gly mutant (TpCS-D317G)
    Depositor
    Insert
    citrate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-17 to phenylalanine, tryptopha…
    Promoter
    recA
    Available since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
  24. pattB-aubergine-ADH-gf

    Plasmid
    #69448
    Purpose
    genomic fragment of Drosophila aubergine was cloned from w1 background and inserted into pattB plasmid; mutagenesis PCR was used to change the catalytic residue DDH to ADH to express cat-dead Aub
    Depositor
    Insert
    aubergine (aub Fly)
    Use
    Tags
    NA
    Expression
    Insect
    Mutation
    chr2L:10,998,626(−)T>G
    Promoter
    aubergine
    Available since
    Oct. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
  25. 4ROH

    Plasmid
    #64659
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    ITCH:JMC081-G03:C229184 (ITCH Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    April 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
  26. GPX5

    Plasmid
    #38875
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    GPX5 (Gpx5 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  27. pUASt- Crb intraGFP

    Plasmid
    #67525
    Purpose
    Expression of transmembrane and the intracellular domains of Crb with GFP.
    Insert
    crumbs (crb Fly)
    Use
    Drosophila
    Tags
    GFP
    Expression
    Insect
    Mutation
    aa97-2103 deleted (extracellular domain)
    Promoter
    hsp70
    Available since
    Aug. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
  28. MAST3

    Plasmid
    #39034
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    MAST3 (MAST3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  29. PECR

    Plasmid
    #39158
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    PECR (PECR Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  30. {Ud}RpL14.Dm

    Plasmid
    #53220
    Purpose
    {Ud} is composed of two genes; (1) a UAS-RpL14.dsRNA targeting RNAi to a haploinsufficient gene RpL14 and (2) an RNAi insensitive RpL14 rescue
    Depositor
    Insert
    RpL14 (RpL14 Fly)
    Use
    Attb drosophila melanogaster transformation
    Tags
    Expression
    Mutation
    14 synonymous mutations introduced
    Promoter
    endogenous promoter
    Available since
    Nov. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
  31. MCEE

    Plasmid
    #39082
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    MCEE (MCEE Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  32. pAAV asyn S87D/S129A

    Plasmid
    #36071
    Insert
    alpha-synuclein S87D/S129A (SNCA Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S87D/S129A
    Promoter
    CMV
    Available since
    Sept. 10, 2012
    Availability
    Academic Institutions and Nonprofits only
  33. CDKL2

    Plasmid
    #39184
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Insert
    CDKL3 (CDKL3 Human)
    Use
    Baculovirus expression
    Tags
    His Tev
    Expression
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  34. pCMV4a-hFJX1-Flag

    Plasmid
    #46931
    Purpose
    expression of human FJX1-FLAG in mammalian cells
    Insert
    FJX1 (FJX1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
  35. SYNJ2BP

    Plasmid
    #38881
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    SYNJ2BP (SYNJ2BP Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  36. pAAV-hSyn-mem-seTurboID

    Plasmid
    #237888
    Purpose
    AAV vector encoding membrane-tethered seTurboID under the control of human synapsin promoter
    Depositor
    Insert
    mem-seTurboID
    Use
    AAV
    Tags
    GAP43 palmitoylation signal and V5 tag
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin 1 (hSyn)
    Available since
    Aug. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
  37. pAAV-CAG-DIO-mem-seTurboID

    Plasmid
    #237887
    Purpose
    AAV vector for Cre-dependent expression of membrane-tethered seTurboID
    Depositor
    Insert
    mem-seTurboID
    Use
    AAV and Cre/Lox
    Tags
    FLAG tag and GAP43 palmitoylation signal
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
  38. pDisplay-CMV-GRAB_LoX3-mut

    Plasmid
    #234651
    Purpose
    This plasmid encodes a mutated version of the genetically encoded chemokine biosensor LoX3-1.0, which is designed to be non-responsive to chemokine binding.
    Depositor
    Insert
    GPCR activation-based mutant chemokine CXCL9-11 sensor GRAB_LoX3-mut
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
  39. pNL [NlucP/IFNb/Hygro] Vector

    Plasmid
    #236928
    Purpose
    Express IFNb in luciferase reporter assay
    Has Service
    DNA
    Insert
    IFNb (IFNB1 Human)
    Use
    Luciferase
    Tags
    NanoLuc (R)
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 5351 - 5400 of 8862 results