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AAVS1-SA-CAG-Pa01-BFP

Plasmid

#234142

Purpose

Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci

Depositor

Gary Hon

Article

Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

Insert

Pa01

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Cluc-NHP.RMA

Plasmid

#227888

Purpose

Expresses Cluc-NHP.RMA under the hSyn promoter. Cluc-NHP.RMA for monitoring neuronal transduction.

Depositor

Jerzy Szablowski

Article

Lee et al bioRxiv 2025.06.01.657212; doi: 10.1101/

Insert

Cypridina luciferase fused to macaque Fc

Use

AAV and Luciferase

Tags

Cluc, IgG1 Fc, and T7 tag

Expression

Mammalian

Mutation

Promoter

Available since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-HaloDA1.0

Plasmid

#234408

Purpose

Expresses the chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0 in neurons

Depositor

Yulong Li

Article

Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

Insert

GPCR activation based chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

Plasmid

#236406

Purpose

Expresses cytosolic low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-DIO- alpha-MSH

Plasmid

#239044

Purpose

Overexpression of alpha-MSH

Depositor

Qingchun Tong

Article

Xu et al Cell Rep. 2023 May 30;42(5):112502. doi:

Insert

alpha-MSH (Pomc Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-DIO-beta-Endorphin

Plasmid

#239043

Purpose

Overexpression of beta endorphin

Depositor

Qingchun Tong

Article

Xu et al Cell Rep. 2023 May 30;42(5):112502. doi:

Insert

beta-Endorphin (Pomc Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Grx1-RoTq-On

Plasmid

#234467

Purpose

Mammalian expression of Grx1-RoTq-On

Depositor

Gary Yellen

Article

Rosen et al Proc Natl Acad Sci U S A. 2025 May 13;12

Insert

Grx1-RoTq-On

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-IgKL-SpyCatcher003-GINKO2

Plasmid

#234544

Purpose

to express a potassium sensor tagged with SpyCatcher in the liver

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

IgKL-SpyCatcher003-GINKO2

Use

AAV

Tags

Expression

Mutation

None

Promoter

P3

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-IgKL-SpyCatcher003-mNeonGreen

Plasmid

#234543

Purpose

to express a green fluorescent protein tagged with SpyCatcher in the liver

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

IgKL-SpyCatcher003-mNeonGreen

Use

AAV

Tags

Expression

Mutation

None

Promoter

P3

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-IgKL-SpyCatcher003-SEP

Plasmid

#234541

Purpose

to express a pH sensor tagged with SpyCatcher in the liver

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

IgKL-SpyCatcher003-SEP

Use

AAV

Tags

Expression

Mutation

None

Promoter

P3

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1 EGFP-p62 HRD

Plasmid

#207550

Purpose

Homologous recombination donor to integrate and expression cassette for eGFP-p62 in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible EGFP-P62

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-L7-6-DD-T6B-MUT-YFP

Plasmid

#235146

Purpose

Expresses the inducible mutant T6B peptide fused with DHFR and YFP under the Purkinje cell-specific L7-6 promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

DHFR-fused mutant T6B peptide

Use

AAV

Tags

Expression

Mutation

5 tryptophan residues of T6B-WT mutated

Promoter

L7-6

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-CAG_G-PTEN

Plasmid

#227438

Purpose

An AAV backbone for expression of the G-PTEN sensor under the CAG promoter, in a Cre dependent manner.

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

G-PTEN sensor (Pten Rat)

Use

AAV

Tags

CD-sREACh and mEGFP

Expression

Mammalian

Mutation

R14G

Promoter

pCAG

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-L7-6-FLAG-HA-T6B-WT-YFP

Plasmid

#235141

Purpose

Expresses the T6B peptide fused with YFP under the Purkinje cell-specific L7-6 promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

T6B peptide

Use

AAV

Tags

FLAG/HA

Expression

Mutation

Promoter

L7-6

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-DIO-Flpo

Plasmid

#237222

Purpose

For Cre-dependent expression of Flp recombinase

Depositor

Thomas Hnasko

Article

Oriol et al bioRxiv [Preprint]. 2025 Jan 17:2024.06.

Insert

Flpo recombinase

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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AAV PHP.eB-CAG-Venus

Plasmid

#233695

Purpose

AAV expression of Venus from CAG promoter

Depositor

Leo James

Article

Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

Insert

Venus

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG_Kinprola_PKA-mEGFP-NLS3__WPRE-SV40

Plasmid

#233365

Purpose

CAG driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for mammalian cell expression through AAV transduction

Depositor

Kai Johnsson

Article

Sun et al bioRxiv 2024.09.11.611894

Insert

Kinprola_PKA-mEGFP-NLS3X

Use

AAV

Tags

mEGFP-NLS3X

Expression

Mutation

Promoter

CAG

Available since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR(RfxCas13d)-CAG-CasRx-pa

Plasmid

#233035

Purpose

To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a CAG promoter

Depositor

Jonathan Ploski

Insert

RfxCas13d-compatible gRNA expression cassette and CasRx

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG_NES-Kinprola_PKA-mTagBFP2_WPRE-SV40

Plasmid

#233364

Purpose

CAG driven expression of the PKA activity recorder Kinprola_PKA fused to mTagBFP2 for mammalian cell expression through AAV transduction

Depositor

Kai Johnsson

Article

Sun et al bioRxiv 2024.09.11.611894

Insert

NES-Kinprola_PKA-mTagBFP2

Use

AAV

Tags

NES and mTagBFP2

Expression

Mutation

Promoter

CAG

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_NES-Kinprola_on-mEGFP_WRPE-SV40

Plasmid

#233368

Purpose

hSyn1 driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Sun et al bioRxiv 2024.09.11.611894

Insert

NES-Kinprola_on-mEGFP

Use

AAV

Tags

NES and mEGFP

Expression

Mutation

Promoter

hSyn1

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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