165,075 results

1. pEN765 -pCAGGS-3XFLAG-mKate2-(human)PDS5B-bpA

   Plasmid

   #156436

   Purpose

   For transient expression of human PDS5B tagged with mKate2

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   human PDS5B (PDS5B Human)
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPD304 VPR-ZF43

   Plasmid

   #138840

   Purpose

   Constitutive expression of VPR-ZF43 under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VPR-ZF43
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW-Flag-CtIP

   Plasmid

   #71108

   Purpose

   mammalian expession of Flag-CtlP

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   CtlP
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Please see Depositor Comments

   Promoter

   TRE promoter, Tet ON

   Available Since

   Nov. 11, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. TFORF3164

   Plasmid

   #144640

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RUVBL1 (RUVBL1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMBP-FnCas12a

   Plasmid

   #113432

   Purpose

   Expresses 10xHis-MBP fused to FnCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   FnCas12a
   

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRedF

   Plasmid

   #118057

   Purpose

   GBPart - RedF Luciferase CDS

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   Red Firefly Luciferase CDS
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Mutation

   Red mutant S286Y

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX304_zeo_empty

   Plasmid

   #160093

   Purpose

   Control lentiviral vector for mammalian gene expression

   Depositor

   Rizwan Haq

   Article

   Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPD547 ZF43x6-Compact_EYFP

   Plasmid

   #138861

   Purpose

   EYFP reporter vector for the ZF43x6-Compact promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x6-Compact
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   ZF43x6-Compact

   Available Since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-WDR12

   Plasmid

   #116803

   Purpose

   Lentiviral expression of WDR12

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   WDR12 (WDR12 Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. vc241

    Plasmid

    #193453

    Purpose

    Expression of rRFP in A. castellanii

    Depositor

    Chantal Abergel

    Article

    Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

    Insert

    mRFP
    

    Use

    Acanthamoeba exression

    Promoter

    GAPDH

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223-MAPK3

    Plasmid

    #23509

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    MAPK3 (MAPK3 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pCRISPaint-mNeon-PuroR

    Plasmid

    #174090

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    mNeonGreen
    

    Use

    Gene tagging

    Available Since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. ER-B

    Plasmid

    #209870

    Purpose

    Dimerization dependent fluorescent protein B anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Tags

    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTHSSe_48

    Plasmid

    #109246

    Purpose

    pSC101 var6 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Expression

    Bacterial

    Promoter

    PJ23105

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBWB419

    Plasmid

    #140637

    Purpose

    RSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.

    Depositor

    Keith Tyo

    Article

    Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

    Insert

    Cas9
    

    Expression

    Bacterial

    Available Since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. ADSL_pLX307

    Plasmid

    #98311

    Purpose

    Lentiviral expression of ADSL

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    ADSL (ADSL Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. ppyCAG_RNaseH1_WT

    Plasmid

    #111906

    Purpose

    Express RNASEH1 in mammalian cell.

    Depositor

    Xiang-Dong Fu

    Article

    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

    Insert

    RNASEH1 (RNASEH1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    July 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. EcMscL

    Plasmid

    #92418

    Purpose

    E. coli Mechanosensitive Channel of Large Conductance

    Depositor

    Douglas Rees

    Insert

    MscL (mscL E. coli)
    

    Tags

    6x Histidine Tag and Thrombin Cleavage Site betwe…

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET His6 Sumo TEV LIC cloning vector (1S)

    Plasmid

    #29659

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    6xHis, SUMO, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCSIIbleo-iRFP670-P2A-EGFP

    Plasmid

    #198060

    Purpose

    The lentiviral vector for iRFP670 and EGFP

    Depositor

    Michiyuki Matsuda

    Insert

    iRFP670
    

    Use

    Lentiviral

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV.CMV.PI.EGFP.WPRE.bGH

    Plasmid

    #105530

    Purpose

    AAV expression of EGFP from CMV promoter

    Depositor

    James M. Wilson

    Has Service

    AAV PHP.eB, AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3_QLuc

    Plasmid

    #183260

    Purpose

    Express QLuc in mammalian cells

    Depositor

    Huiwang Ai

    Article

    Xiong et al J Am Chem Soc. 2022 Aug 1. doi: 10.1021/

    Insert

    QLuc
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLenti-IFT20-EGFP

    Plasmid

    #118032

    Purpose

    Expresses IFT20-EGFP in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Insert

    human IFT20 (IFT20 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    EF1 alpha

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAcGFP1-N1-RvCAHS12-GS

    Plasmid

    #192462

    Purpose

    Expression of RvCAHS12-GS_linker-AcGFP1 in mammalian cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS12
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pHR-CMV-TetO2_3C-Twin-Strep

    Plasmid

    #113883

    Purpose

    Mammalian (inducible) protein expression

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Twin-Strep

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLenti-H3.3(WT)-3AID-HA-2A-mCherryBSD

    Plasmid

    #225697

    Purpose

    Lentiviral expression of AID degron tagged Wildtype H3.3 and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (WT) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xAID HA and T2A-mCherry

    Expression

    Mammalian

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pTP396

    Plasmid

    #149336

    Purpose

    Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

    Depositor

    Rebecca Voorhees

    Article

    Pleiner et al Science. 2020 May 21. pii: science.abb50

    Insert

    anti-GFP nanobody
    

    Tags

    Biotin acceptor peptide (Avi-tag), His14, and SUM…

    Expression

    Bacterial

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET28b_H6-Smt3-AesC-GFP

    Plasmid

    #234486

    Purpose

    Superfolder GFP C-terminally fused to His-Smt3-tagged AesC split intein

    Depositor

    Henning Mootz

    Article

    Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

    Insert

    C-terminal Fragment of the Aes123 PolB1 Split Intein
    

    Tags

    Hexahistidine Tag, Superfolder Green Fluorescent …

    Expression

    Bacterial

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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29. pDsRed-Sensor

    Plasmid

    #22743

    Depositor

    Lynn Hudson

    Article

    Lau et al J Neurosci. 2008 Nov 5. 28(45):11720-30.

    Insert

    modified pCMV-DsRed-Express (Clontech)
    

    Expression

    Mammalian

    Available Since

    Dec. 18, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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30. canxTM-mEmerald (cytosolic)

    Plasmid

    #186962

    Purpose

    Expresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    CANX (CANX Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Only express calnexin (482-end) and add ER signal…

    Promoter

    CMV Promoter

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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