169,691 results
-
Plasmid#177591PurposeExpresses His tagged LeuRS in Escherichia coli cellsDepositorInsertLeucine tRNA synthetase
TagsHexahistidineExpressionBacterialAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pPig_8X-TOPFLash-tdIRFP_Puro
Plasmid#249713PurposeTopFlash reporter with puromycin. Used as a live readout of downstream Wnt gene transcription.DepositorInsertstdiRFP
T2A-PuroR
ExpressionMammalianPromoterTopFlash and TopFlash, with 7x TCF/LEF motifAvailable SinceJan. 14, 2026AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1α-H2B-PA-mCherry-PGKneo
Plasmid#247340PurposeExpresses photoactivatable H2B-Cherry under EF1 promoter and can be randomly integrated by PiggyBac transposaseDepositorAvailable SinceNov. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-SUMO-h-cGAS(T321I)
Plasmid#232294PurposeExpression of mutant human cGAS protein (T321I)DepositorInsertMB21D1 (CGAS )
MutationT321IAvailable SinceAug. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP-P2A-mCherry
Plasmid#229604PurposeExpresses EGFP and mCherry in mammalian cellsDepositorInsertEGFP
UseAAVExpressionMammalianAvailable SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
TFORF1164
Plasmid#144274PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
Const.6 EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc-PS9
Plasmid#231910PurposeFor packaging EGFP, dTomato, TagBFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)DepositorInsertEGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc
ExpressionMammalianPromoterCMVAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHBJT034
Plasmid#225180PurposeLow copy cloning vector with mCherry integrated in the multiple cloning site (SmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.DepositorTypeEmpty backboneUseSynthetic BiologyTagsmCherryExpressionBacterialAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT023
Plasmid#225174PurposeLow copy cloning vector with same multiple cloning site as pUC19 and pSU19 (SmR).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Arg13Ala
Plasmid#208172PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 Arg13Ala mutant (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
TagsStrep-tagExpressionMammalianMutationArg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…Available SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Arg300Ala
Plasmid#208173PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg300Ala (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys,Arg300Ala)
TagsStrep-tagExpressionMammalianMutationLeu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe19…Available SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Lys15Ala
Plasmid#208174PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Lys15Ala (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
TagsStrep-tagExpressionMammalianMutationLys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…Available SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-sTagRFP-TUBA1B
Plasmid#207765PurposeDonor template for sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorInsertTUBA1B Homology Arms flanking a sTagRFP Tag (TUBA1B Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
vYM245_PB-Blast-pEF1a-H2B-mCh-D3bCI
Plasmid#202596Purposeplasmid for expressing the DNMT3bCI (catalytic inactive)DepositorInsertH2B-mCherry-T2A-rTetR-DNMT3bCI (Dnmt3b Synthetic, Mouse)
UseSynthetic BiologyTagsrTetRExpressionMammalianMutationP656V, C657DAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1915
Plasmid#143860PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-PAH1-3HA
Plasmid#184761PurposePAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.DepositorAvailable SinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-CDS1-3HA
Plasmid#184760PurposeCDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.DepositorAvailable SinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJC20_cam1-T6C
Plasmid#182427PurposeBacterial expression of the Schizosaccharomyces pombe fission yeast calmodulin, Cam1 with the T6C residue replacement to allow labellingDepositorAvailable SinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40-Asp115Ala
Plasmid#180760PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)DepositorInsertα-COPI-WD40-Asp115Ala
TagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Asp115Ala, Leu…PromoterCMVAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-Tollip
Plasmid#178050PurposeGFP fused to human Tollip cDNA. Localisation and expression in mammalian cells.DepositorAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 mito-TagRFP-T
Plasmid#174543Purposelabels mitochondria with TagRFP-TDepositorInsertMTS-tagRFP-T
UseLentiviralTagstagRFP-TExpressionMammalianAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8m
Plasmid#162372PurposeMammalian expression of ultrafast protein calcium sensorDepositorInsertjGCaMP8m
Tags6xHisExpressionMammalianMutationA25G F286YPromoterCMVAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-oNGN2
Plasmid#198397PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.InsertsTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationS24A, S193A, S207A, S209A, S219A, S232A, S239A, S…PromoterCAG, EF1a, and TRE3GAvailable SinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Δ18
Plasmid#164436PurposeEncodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV-2 Spike (S )
ExpressionMammalianAvailable SinceFeb. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-hAXL
Plasmid#124797PurposeTet-inducible AXL expression in mammalian cellsDepositorAvailable SinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-EK_C122S_His5
Plasmid#49048PurposeExpresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coliDepositorInsertEnteropeptidase, peptidase domain (TMPRSS15 Bovine)
Tags5xHisExpressionBacterialMutationP92R, E185D, C122SPromoterT7Available SinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-UTX
Plasmid#24168PurposeMammalian expression of human UTX with HA tagDepositorAvailable SinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCIS GEM-CEPIA1er
Plasmid#58217PurposeRatiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulumDepositorInsertGEM-CEPIA1er
TagsmycExpressionMammalianMutationGEM-GECO1 E298D F359W D400E E407DPromoterCAGAvailable SinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGH125_dCas9-Blast
Plasmid#85417Purposelentiviral expression vector for dCas9 with Blasticidin selectable markerDepositorInsertdCas9 and Blasticidin resistance
UseCRISPR and LentiviralExpressionMammalianAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
phU6-gRNA
Plasmid#53188PurposeExpresses the S. pyogenes sgRNA from the human U6 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRExpressionMammalianPromoterhuman U6Available SinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only