We narrowed to 7,473 results for: Lif

1. kanMX6-ins1

   Plasmid

   #195038

   Purpose

   pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tDEG1), allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-sgCtrl_EF1a-Puro-T2A-BFP

   Plasmid

   #195500

   Purpose

   Lentiviral BFP expression vector bearing a sgRNA targeting a randomized human TSS sequence

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP, Puro, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sgT-REX17_EF1a-Puro-T2A-BFP

   Plasmid

   #195501

   Purpose

   Lentiviral BFP expression vector bearing a sgRNA targeting the promoter of human T-REX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP, Puro, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV_Syn_Xph18_eGFP_CCR5TC

   Plasmid

   #187443

   Purpose

   Encodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph18
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV_Syn_Xph15_eGFP_CCR5TC

   Plasmid

   #187442

   Purpose

   Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph15
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. fru-T2A-CsChrimson-tdTomato HDR plasmid

   Plasmid

   #141100

   Purpose

   Plasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru gene

   Depositor

   Leslie Vosshall

   Article

   Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10

   Insert

   fru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm
   

   Use

   Donor template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFUGW-shRIIα-RIIαS97A-IRES-EGFP

   Plasmid

   #183457

   Purpose

   Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA and Prkar2a (Prkar2a Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   shRNA-resistant mutations: C135>G, G138>A, …

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUGW-shRIIα-RIIαS97E-IRES-EGFP

   Plasmid

   #183458

   Purpose

   Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA and Prkar2a (Prkar2a Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   shRNA-resistant mutations: C135>G, G138>A, …

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJET-22-DExCon-mNeonGreen-R11a

   Plasmid

   #179897

   Purpose

   Donor with homologous arms for Rab11a to knock in DExCon-mNeonGreen module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11A, member RAS oncogene family (RAB11A Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMH011_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid

    #179434

    Purpose

    Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-HS4-mch (SH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSL006_phiC31_neo-5xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry

    Plasmid

    #179425

    Purpose

    Dual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-(nospacer)-mch
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV9-hSyn-iDianiSnFR_PM

    Plasmid

    #177759

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    pAAV9-hSyn-iDianiSnFR_PM
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV9-hSyn-iDianiSnFR_ER

    Plasmid

    #177758

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    pAAV9-hSyn-iDianiSnFR_ER
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV9-hSyn-iCyt_F_SnFR_PM

    Plasmid

    #177755

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    pAAV9-hSyn-iCyt_F_SnFR_PM
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV9-hSyn-iCytSnFR_PM

    Plasmid

    #177753

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    pAAV9-hSyn-iCytSnFR_PM
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV9-hSyn-iCytSnFR_ER

    Plasmid

    #177752

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    pAAV9-hSyn-iCytSnFR_ER
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Anti-TRIP8b (constant) [N212/7R]

    Plasmid

    #177514

    Purpose

    Mammalian Expression Plasmid of anti-TRIP8b (constant) (Rat). Derived from hybridoma N212/7.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-TRIP8b (constant) (Rattus norvegicus) recombinant mouse monoclonal antibody (Pex5l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV9-hSyn iCyt_BrEt_SnFR_ER

    Plasmid

    #177756

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    pAAV9-hSyn iCyt_BrEt_SnFR_ER
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV9-hSyn-iCyt_F_SnFR_ER

    Plasmid

    #177754

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    pAAV9-hSyn-iCyt_F_SnFR_ER
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pME-MmCavin4

    Plasmid

    #170528

    Purpose

    Entry clone for tagging MmCavin4 at the C terminus. Parton lab clone ADM

    Depositor

    Rob Parton

    Article

    Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

    Insert

    Cavin4 (Cavin4 Mouse)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pME-MmCav1

    Plasmid

    #170527

    Purpose

    Entry clone for tagging MmCav1 at the C terminus. Parton lab clone BCJ

    Depositor

    Rob Parton

    Article

    Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

    Insert

    Caveolin1 (Cav1 Mouse)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAW-BiP-Nb127D01-mCherry-KDEL

    Plasmid

    #171575

    Purpose

    Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    BiP-Nb127D01-mCherry-KDEL (CXCR2 Human)
    

    Use

    Tags

    BiP signal peptide and mCherry-KDEL

    Expression

    Insect

    Mutation

    Promoter

    fly actin5C promoter

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAW-BiP-NbVHH05-mCherry-KDEL

    Plasmid

    #171574

    Purpose

    Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    BiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)
    

    Use

    Tags

    BiP signal peptide and mCherry-KDEL

    Expression

    Insect

    Mutation

    Promoter

    fly actin5C promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV ShadowY-AURKA-mTurq2

    Plasmid

    #157770

    Purpose

    Expression of AuroraA kinase biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    mTurquoise2 and shadowY

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA 3.1 mitoAURKA Lys162Met

    Plasmid

    #157752

    Purpose

    Expression of kinase-dead AURKA K62M in mitochondria

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP N1 AURKA 1-110

    Plasmid

    #157755

    Purpose

    Expression of AURKA Nter (aa1-110):GFP in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The first 110 amino acids of AURKA were cloned

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP N1 AURKA 1-30

    Plasmid

    #157756

    Purpose

    Expression of AURKA Nter (aa1-30):GFP in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The first 30 amino acids of AURKA were cloned

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA 3.1 AURKA deltaNter

    Plasmid

    #157749

    Purpose

    Expression of AURKA without Nter in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    the first 30AA of Aurora A are lacking

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Anti-TRIP8b [N212/7R]

    Plasmid

    #164154

    Purpose

    Mammalian Expression Plasmid of anti-TRIP8b (Rat). Derived from hybridoma N212/7.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-TRIP8b (Rattus norvegicus) recombinant mouse monoclonal antibody (Pex5l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Anti-Iduna/RNF146 [N201/35R]

    Plasmid

    #164157

    Purpose

    Mammalian Expression Plasmid of anti-Iduna/RNF146 (Mouse). Derived from hybridoma N201/35.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Iduna/RNF146 (Mus musculus) recombinant mouse monoclonal antibody (Rnf146 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge