We narrowed to 7,357 results for: Ank
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Plasmid#112371PurposeCRISPR donor plasmid to tag human transcription factor ELF4 with GFPDepositorInsertELF4 homology arms flanking EGFP-IRES-Neo cassette (ELF4 Human)
UseCRISPRAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVEZF1-donor
Plasmid#112341PurposeCRISPR donor plasmid to tag human transcription factor VEZF1 with GFPDepositorInsertVEZF1 homology arms flanking EGFP-IRES-Neo cassette (VEZF1 Human)
UseCRISPRMutationrs999907002, hg38:17:57975308:A>T, hg38:17:579…Available SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNR1D2-donor
Plasmid#112314PurposeCRISPR donor plasmid to tag human transcription factor NR1D2 with GFPDepositorInsertNR1D2 homology arms flanking EGFP-IRES-Neo cassette (NR1D2 Human)
UseCRISPRMutationhg38:3:23977483:A>T, hg38:3:23977515:A>G, h…Available SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPS3911
Plasmid#113330Purposecontrol for Hermes transposition; TIR-flanked LEU2, transposase absentDepositorInsertbeta-isopropyl malate dehydrogenase
ExpressionYeastAvailable SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNJB99
Plasmid#51521PurposeGateway entry vector with attL1 and attR5 sites flanking Zif268:FokI coding sequenceDepositorInsertZif268:FokI
UseGateway entry vectorTagsNLSAvailable SinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pimEJ5GFP
Plasmid#44026DepositorInsertEJ5GFP egfp-based chromosomal break reporter
ExpressionMammalianMutationpCAGGS promoter and eGFP separated by pgkPURO cas…PromoterpCAGGSAvailable SinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTVGFP
Plasmid#169445PurposeA vector with two different MCSs flanking an attP integration site and a floxable pax-GFP markerDepositorTypeEmpty backboneUseTargeting vectorAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHEN2-SG4
Plasmid#196071PurposeExpress wild type SG4 in E.coliDepositorInsertSG4
Tags3XFLAG and 6XHisExpressionBacterialPromoterT7Available SinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAM_Ec
Plasmid#102939PurposeTransposon mutagenesis and Tn-seqDepositorInsertmariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
ExpressionBacterialAvailable SinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain
Plasmid#228663PurposeBacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)DepositorInsertZika virus portion of NS2B protease coexpressed with NS3 protease
TagsHis-GST-TEVExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
WT FL USP7
Plasmid#227214PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialPromoterT7-promoterAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor_mU6
Plasmid#69350PurposesgRNA scaffold and mouse U6 promoterDepositorInsertmU6 promoter and sgRNA scaffold flanked by BbsI sites
UseCRISPRPromotermU6 promoterAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag
Plasmid#204816PurposeBacterial expresison of Enterovirus A71 3C proteaseDepositorInsertEV A71 3C protease
ExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCfB2224
Plasmid#67547PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease fusion with non cleavable linker (N-terminal Twin-Strep-tag)
Plasmid#228661PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Zika virus for biochemical assayDepositorInsertZika virus portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site
Plasmid#228633PurposeBacterial expression of the A71 2A protease with ITTL at the N-terminal and a C110A mutation used for crystallization in P 6 1 (PDB 9FGO)DepositorInsertEnterovirus Coxsackievirus A71 2A protease
TagsHis-SUMOExpressionBacterialMutationC110AAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
DENV2 NS2B-NS3 protease: Co-expression of DENV-2 NS2B and NS3
Plasmid#204808PurposeBacterial co-expression of Dengue2 NS2B and NS3 proteinsDepositorInsertDengue2 NS2B (POLY Dengue2)
ExpressionBacterialAvailable SinceDec. 18, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL4.75-KRAS LCS6m
Plasmid#44571DepositorInsertKRAS 3'UTR (KRAS Human)
ExpressionMammalianMutationThe T allele at LCS6 (let-7 complementary site 6)…PromoterCMVAvailable SinceApril 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Tm2AG
Plasmid#26773DepositorInsertTdTomato-2A-H2BGFP-SV40pA
ExpressionMammalianMutationmutation in 2A peptide that renders it non-functi…Available SinceMarch 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNR4A1-donor
Plasmid#112295PurposeCRISPR donor plasmid to tag human transcription factor NR4A1 with GFPDepositorInsertNR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)
UseCRISPRMutationhg38:12:52058840:C>T, hg38:12:52058930:A>C,…Available SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pESR1-donor
Plasmid#112337PurposeCRISPR donor plasmid to tag human transcription factor ESR1 with GFPDepositorAvailable SinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
dg263_pENTR_ChrimsonCel
Plasmid#66101PurposeEntry clone containing Chrimson coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2DepositorInsertChrimson
UseGatewayAvailable SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
PRExpress
Plasmid#122486PurposeA non-leaky heat-inducible gene expression system for Drosophila. (GenBank: MF058598.1)DepositorInsertsLacZ
poly-PRE
ExpressionInsectAvailable SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
dg265_pENTR_CoChRCel
Plasmid#66103PurposeEntry clone containing CoChR coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2DepositorInsertCoChR
UseGatewayAvailable SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB2193
Plasmid#67542PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.DepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
Degron-KI donor backbone
Plasmid#65483PurposeDonor backbone vector to clone left and right HR arms in for Degron-KI applicationDepositorTypeEmpty backboneAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
dg264_pENTR_ChronosCel
Plasmid#66102PurposeEntry clone containing Chronos coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2DepositorInsertChronos
UseGatewayAvailable SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-mScarlet
Plasmid#191324PurposeExpresses fluorescent labeled beta Tubulin to measure microtubule densityDepositorInsertTUBB2a-mScarlet (Tubb2a Mouse)
ExpressionMammalianAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
EV-D68 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag
Plasmid#204817PurposeBacterial expresison of Enterovirus D68 3C proteaseDepositorInsertEV D68 3C protease
ExpressionBacterialAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRVV661
Plasmid#108282PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertPhiC31 attP sites
UseCRISPRAvailable SinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5
Plasmid#61255PurposeCRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassetteDepositorInsert2xTY1-V5
UseCRISPRTagsTY1 and V5Available SinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRVV669
Plasmid#108283PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertPhiC31 attP sites
UseCRISPRAvailable SinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3xP3-EGFP.vas-int.NLS
Plasmid#60948PurposeDrosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.DepositorInsertvas-phiC31 integrase.NLS
UseRmcePromotervasaAvailable SinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 2A protease; aliases: Enterovirus EV71 2A protease, 6xHis-SUMO-TEV tag, self cleaving
Plasmid#204810PurposeBacterial expresison of Enterovirus A71 2A proteaseDepositorInsertA71 2A protease
ExpressionBacterialAvailable SinceDec. 18, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSKB-
Plasmid#62540PurposeCloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate CloningDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCHAP2
Plasmid#206847PurposeA plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP1
Plasmid#206846PurposeA plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB2223
Plasmid#67544PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2196
Plasmid#67549PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked bleMX marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-CII
Plasmid#51566PurposeExpression of bacteriophage lambda CII for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertcII
TagsIntein-Chiting Binding DomaninExpressionBacterialAvailable SinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB2399
Plasmid#67550PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked amdSYM marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V5
Plasmid#61257PurposeCRISPR-RMCE, second step attB plasmid, intron phase 2, exchange dsRed with 2xTY1 and V5 tags cassetteDepositorInsert2xTY1-V5
UseCRISPRTagsTY1 and V5Available SinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
EV-D68 2A protease; aliases: Enterovirus D68 2A protease, 6xHis-tag, low expression levels
Plasmid#204801PurposeBacterial expresison of Enterovirus D68 2A proteaseDepositorInsertD68 2A protease
ExpressionBacterialAvailable SinceDec. 11, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pES133
Plasmid#127552PurposeVariant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13DepositorInsertF5-flanked tetracycline resistance cassette
ExpressionBacterialMutationMultiple SNPs in tetA (originally from pBR322) to…Available SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-STK32C_IDG-K
Plasmid#170610PurposeGateway destination clone of STK32C (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-STK32C (STK32C Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianPromoterUbiquitinAvailable SinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-1-2xTY1-V5
Plasmid#61256PurposeCRISPR-RMCE, second step attB plasmid, intron phase 1, exchange dsRed with 2xTY1 and V5 tags cassetteDepositorInsert2xTY1-V5
UseCRISPRTagsTY1 and V5Available SinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKG113
Plasmid#63073PurposeRetroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)DepositorInsertCENP-T (CENPT Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationE. coli codon optimized. G217R mutation compared …Available SinceJan. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-mNeonGreen
Plasmid#191323PurposeExpresses fluorescent labeled beta Tubulin to measure microtubule densityDepositorInsertTUBB2a-mNeonGreen (Tubb2a Mouse)
ExpressionMammalianAvailable SinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFOXF2-donor
Plasmid#113816PurposeCRISPR donor plasmid to tag human transcription factor FOXF2 with GFPDepositorInsertFOXF2 homology arms flanking EGFP-IRES-Neo cassette (FOXF2 Human)
UseCRISPRAvailable SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-STK32A_IDG-K
Plasmid#170608PurposeGateway destination clone of STK32A (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-STK32A (STK32A Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianPromoterUbiquitinAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only