We narrowed to 148,810 results for: ins;

1. pJW6.01

   Plasmid

   #62882

   Purpose

   Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR

   Depositor

   Geraldine Seydoux

   Article

   Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

   Insert

   GFP::MEG-3
   

   Tags

   GFP

   Expression

   Worm

   Promoter

   meg-3

   Available Since

   March 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCEP4-hKCNE1

   Plasmid

   #53050

   Purpose

   Expresses beta subunit for IKs channel in mammalian cells

   Depositor

   Michael Sanguinetti

   Article

   Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

   Insert

   Potassium voltage-gated channel subfamily E member 1 (KCNE1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC57-Amp_CRISPIE donor B2

   Plasmid

   #172843

   Purpose

   CRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-0) encoding mRuby3
   

   Use

   CRISPR

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-KS-attB1-2-PT-SA-SD-1-GFP11

   Plasmid

   #172068

   Purpose

   Protein trap plasmid for inserting GFP11 into a phase 1 coding intron

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   GFP11x7
   

   Expression

   Insect

   Available Since

   Oct. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pFX-mKate2-Rab11

   Plasmid

   #174463

   Purpose

   Express ｍKate2 with recycling endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mKate2 with recycling endosome-targeting sequence
   

   Tags

   small GTPase (Rab11)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. DT240

   Plasmid

   #80414

   Purpose

   Expresses 240 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

   Depositor

   Thomas Cooper

   Article

   Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

   Insert

   human DMPK exons 11-15 with 240 interrupted CTG repeats (DMPK Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFX-mito-eqFP650

   Plasmid

   #174179

   Purpose

   Express eqFP650 with mitochondria-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   eqFP650 with mitochondria-targeting sequence
   

   Tags

   the mitochondria-targeting sequence of the cytoch…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-4T-2/hGGA3(GAT)

   Plasmid

   #79436

   Purpose

   Expresses GST-tagged GAT domain of GGA3 (107-286 aa) in E.coli

   Depositor

   Kazuhisa Nakayama

   Article

   Takatsu et al Biochem J. 2002 Jul 15;365(Pt 2):369-78.

   Insert

   GGA3 (GGA3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 107-286

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFX-mCherry-Rab11

   Plasmid

   #174461

   Purpose

   Express mCherry with recycling endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with recycling endosome-targeting sequence
   

   Tags

   small GTPase (Rab11)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFX-mCherry-Rab7

    Plasmid

    #174458

    Purpose

    Express mCherry with late endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with late endosome-targeting sequence
    

    Tags

    small GTPase (Rab7)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFX-EGFP-Sec61β

    Plasmid

    #174466

    Purpose

    Express EGFP with endoplasmic reticulum-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Sirius with endoplasmic reticulum-targeting sequence
    

    Tags

    Sec61 subunit beta

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJL1 Aquamarine

    Plasmid

    #106285

    Purpose

    Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    Aquamarine
    

    Tags

    6xHis and TEV cleavage (N terminal on insert)

    Expression

    Bacterial

    Mutation

    T65S and H148G

    Promoter

    T7

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET17b-yebST

    Plasmid

    #175804

    Purpose

    Complementation of yebST knockout in E. coli K-12

    Depositor

    Gira Bhabha, Damian Ekiert, Ian Henderson

    Article

    Isom et al Sci Rep. 2017 Aug 17;7(1):8608. doi: 10.

    Insert

    yebST
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    silent mutations to introduce restriction sites

    Promoter

    T7

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAB076i5

    Plasmid

    #79199

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1+2+3) gIII, luxAB; Ppro1 434cI-SH2ABL1
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (OR1+2+3); Ppro1

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFX-mito-roGFP

    Plasmid

    #174181

    Purpose

    Express roGFP with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    roGFP with mitchondria-targeting sequence
    

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFX-EGFP-Rab11

    Plasmid

    #174460

    Purpose

    Express iRFP with recycling endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with recycling endosome-targeting sequence
    

    Tags

    small GTPase (Rab11)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAB078d8

    Plasmid

    #79206

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1+2) luxAB; Ppro1 434cI-SH2ABL1
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (OR1+2); Ppro1

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET30a-FluA-NP

    Plasmid

    #165092

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Influenza A virus(A/Georgia/03/2019(H1N1) nucleocapsid protein
    

    Expression

    Bacterial

    Available Since

    Feb. 11, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pAB092a

    Plasmid

    #79217

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-SH2ABL1
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (OR1+2); Ppro1

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-Cas13d-T2A-GFP(1-10)

    Plasmid

    #224566

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    Cas13d-T2A-GFP(1-10)
    

    Use

    CRISPR

    Tags

    T2A-split GFP(1-10)

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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