2,202 results

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pCS2+Paxillin-mKate

Plasmid

#105974

Purpose

in vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesions

Depositor

Caren Norden

Article

Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

Insert

Paxillin

Tags

mKate

Expression

Bacterial

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST_6xUAS:mNeonGreen-Rab5a

Plasmid

#192355

Purpose

zfRabaa tagged with mNeonGreen, expressed under the UAS promoter

Depositor

Francesca Peri

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

Rab5a (rab5aa Zebrafish)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab6a

Plasmid

#109652

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab6a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab6a (rab6a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab6ba

Plasmid

#109653

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab6ba

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab6ba (rab6ba Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab6bb

Plasmid

#109654

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab6bb

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab6bb (rab6bb Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus

Plasmid

#105127

Purpose

Used along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent protein

Depositor

Kimberley Evason, Didier Stainier

Article

Evason et al PLoS Genet. 2015 Jul 2;11(7):e1005305. d

Inserts

Zebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)

Use

Zebrafish transgenesis

Promoter

cryaa (crystallin) and fabp10a

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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acvr1l-HA

Plasmid

#201904

Purpose

in vitro transcription of RNA encoding HA-tagged Acvr1l

Depositor

Mary Mullins

Article

Tajer et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

acvr1l (acvr1l Zebrafish)

Tags

HA

Promoter

SP6

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestRFP_RNAseH1

Plasmid

#65785

Purpose

expresses RFP-tagged RNAseH1 in mammalian cells

Depositor

Thomas Tuschl

Insert

RNaseH1 (RNASEH1 Zebrafish, Human)

Tags

RFP and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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ZE25 Stbm-pCS2+

Plasmid

#17067

Depositor

Randall Moon

Article

Park et al Nat Cell Biol. 2002 Jan;4(1):20-5.

Insert

Stbm (vangl2 Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-Pcdh19-GFP

Plasmid

#35675

Depositor

James Jontes

Article

Biswas et al J Cell Biol. 2010 Nov 29;191(5):1029-41.

Insert

Pcdh19 (pcdh19 Zebrafish)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only
pUFlip-floxed2A-KalTA4-; HE1A:BFP -2

Plasmid

#180012

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; HE1A: BFP

Use

Synthetic Biology

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pT2KXIG (Tol2)-tp63:ikk1:GFP

Plasmid

#105646

Purpose

Ikk1(alpha) expression in basal keratinocytes

Depositor

Sandra Rieger

Article

Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

Insert

tp63:ikk1:GFP (chuk Zebrafish)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-EEA1

Plasmid

#109560

Purpose

Multisite gateway vector for 5' tagging with early endosome associated 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

early endosome associated 1 (eea1 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP

Plasmid

#158963

Purpose

Cre-dependet Cas9-GFP expression

Depositor

Michael Brand

Article

Kesavan et al Development. 2020 Jun 8;147(11). pii: de

Inserts

hsp70l promoter
floxed Non-FP cassette
Cas9-GFP

Use

CRISPR and Cre/Lox

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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p5E-mnx1

Plasmid

#82402

Purpose

Gateway-compatible zebrafish mnx1 promoter

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mnx1 promoter

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pcRII-mogat3a in situ probe

Plasmid

#232135

Purpose

zebrafish mogat3a anti-sense in situ probe, linearize with NotI, SP6 polymerase

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

mogat3a

Use

In vitro transcription

Promoter

SP6/T7

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCK033 p5E desma MCS

Plasmid

#195948

Purpose

Gateway p5E vector

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

desmin a regulatory elements (desma Zebrafish)

Use

Other

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2+ centrin-mKate2

Plasmid

#105941

Purpose

in vitro transcription, zf centrin N-terminally tagged with mkate2

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Centrin

Tags

mKate2

Expression

Bacterial

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RNAseH1

Plasmid

#65782

Purpose

expresses FLAGHA-tagged RNAsH1 in mammalian cells

Depositor

Thomas Tuschl

Insert

RNaseH1 (RNASEH1 Zebrafish, Human)

Tags

FLAG HA and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_ect2_cmlc2-nmKate

Plasmid

#238410

Purpose

Drives expression of 3 different gRNAs targeting ect2, and expression of nuclear mKate in cardiomyocytes

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting ect2

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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