2,163 results

1. pME-PLIN2-tdTOMATO

   Plasmid

   #172533

   Purpose

   PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

   Depositor

   Richard White

   Article

   Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

   Insert

   plin2-tdtomato
   

   Use

   Tags

   Expression

   Mutation

   S80R, A81S, E144D, I148F, and R415H mutations in …

   Promoter

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST_6xUAS:miRFP670-cetn4

   Plasmid

   #192357

   Purpose

   zfCETN4 tagged with miRFP670, expressed under the UAS promoter

   Depositor

   Darren Gilmour

   Article

   Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

   Insert

   CETN4 (cetn4 Zebrafish)
   

   Use

   Tags

   miRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCK069 desmaMCS:minprom_mApple,exorh:EGFP

   Plasmid

   #195982

   Purpose

   Expression construct

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   desmin a regulatory elements, mouse beta-globin minimal promoter mApple, exorh:EGFP (desma Zebrafish)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTol2CG2-hsp70I:QFGal4-SV40pA

   Plasmid

   #155120

   Purpose

   Tol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   hsp70l:QFGal4 (hsp70l Zebrafish, Budding Yeast, Neurospora crassa)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   hsp70l

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS

   Plasmid

   #78251

   Purpose

   Can be used to subclone in tagged gene of interest into inducible motor neuron expression vector.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Inserts

   -3mnx1 (mnx1 Zebrafish)
   -loxP-mTagBFP2-stop-
   mCherry-NS-MCS
   

   Use

   Zebrafish motor neuron inducible expression

   Tags

   Expression

   Mutation

   Promoter

   -3mnx1

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+Paxillin-mKate

   Plasmid

   #105974

   Purpose

   in vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesions

   Depositor

   Caren Norden

   Article

   Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

   Insert

   Paxillin
   

   Use

   Tags

   mKate

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tol2-cmlc2-Trpa1b-2A-EGFP

   Plasmid

   #106405

   Purpose

   For expressing Trpa1b-2A-EGFP in zebrafish cardiomyocytes.

   Depositor

   Randall Peterson

   Article

   Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

   Inserts

   cmlc2 promoter
   Trpa1b (trpa1b Zebrafish)
   

   Use

   Gateway

   Tags

   2A-EGFP

   Expression

   Mutation

   Promoter

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_PGK_antiCD19_eZ3_Gal4VP64

   Plasmid

   #169917

   Purpose

   Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

   Depositor

   Zhili Rong

   Article

   Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

   Insert

   antiCD19-Notch3-GL4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Additional EGF repeat between the extracellular d…

   Promoter

   PGK

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTol2CG2-acta1:QFGal4-SV40pA

   Plasmid

   #155121

   Purpose

   Tol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   acta1:QFGal4
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   acta1

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKHR5

    Plasmid

    #74593

    Purpose

    an alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.

    Depositor

    David Grunwald

    Article

    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

    Insert

    FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
    

    Use

    Cre/Lox; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    alpha-crystallin

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. p5'Entry-lck-5.5kb

    Plasmid

    #58890

    Purpose

    provides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenes

    Depositor

    Jeffrey Yoder

    Insert

    zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
    

    Use

    Gateway 5' entry clone

    Tags

    Expression

    Mutation

    mutated single nucleotide (T-->C, 13th nucleot…

    Promoter

    lymphocyte-specific protein tyrosine kinase (lck)…

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST mfap4:tdTomato

    Plasmid

    #135214

    Purpose

    Red tagged macrophages

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Insert

    mfap4 promoter and tdTomato
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. zbFGF

    Plasmid

    #12309

    Depositor

    James Thomson

    Article

    Ludwig et al Nat Methods. 2006 Aug . 3(8):637-646.

    Insert

    Fgf2 (fgf2 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Ala 11 mutated to Pro, Ser 55 mutated to Asn

    Promoter

    Available since

    Sept. 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3E-U6a-U6c-rac2 guide

    Plasmid

    #107600

    Purpose

    An entry vector with U6a and U6c promoter driving rac2 guide RNAs expression

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    rac2 guide RNA sequences
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. shha-pCRII

    Plasmid

    #68962

    Purpose

    for antisense probe, NotI w/ Sp6

    Depositor

    David Traver

    Article

    Clements et al Nature. 2011 Jun 8;474(7350):220-4. doi:

    Insert

    shha (shha Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

16. p5E-mnx1

    Plasmid

    #82402

    Purpose

    Gateway-compatible zebrafish mnx1 promoter

    Depositor

    Judith Eisen

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    mnx1 promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCK033 p5E desma MCS

    Plasmid

    #195948

    Purpose

    Gateway p5E vector

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    desmin a regulatory elements (desma Zebrafish)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPRISM-gal4-VP16-cmlc2-mRFP

    Plasmid

    #117781

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    gal4/VP16
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. ZE11 tiggywinkle hedgehog CMV

    Plasmid

    #17060

    Depositor

    Randall Moon

    Insert

    tiggywinkle hedgehog (shhb Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pDEST-Tol2-PA2-CMV-AB-mCh

    Plasmid

    #160435

    Purpose

    Expresses human Amyloid Beta-mCherry in Zebrafish

    Depositor

    Nicholas Tolwinski

    Article

    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

    Insert

    Human Amyloid Beta peptide (1-42) (APP Zebrafish, Human)
    

    Use

    Zebrafish expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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