2,159 results

1. pTol1:uas-myc-act-map2k2b-2a-tdTomato

   Plasmid

   #104721

   Purpose

   UAS-driven expression of activated MEK

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Insert

   activated map2k2b (map2k2b Zebrafish)
   

   Use

   Zebrafish transposon

   Tags

   2A-tdTomato and myc tag

   Expression

   Mutation

   serines 221 and 225 to aspartic acid

   Promoter

   10xUAS

   Available since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPRISM-Cre-cmlc2-eGFP

   Plasmid

   #117794

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   Cre
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST-Tol2-PA2-CMV-AB-mCh

   Plasmid

   #160435

   Purpose

   Expresses human Amyloid Beta-mCherry in Zebrafish

   Depositor

   Nicholas Tolwinski

   Article

   Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

   Insert

   Human Amyloid Beta peptide (1-42) (APP Human, Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. zbFGF

   Plasmid

   #12309

   Depositor

   James Thomson

   Article

   Ludwig et al Nat Methods. 2006 Aug . 3(8):637-646.

   Insert

   Fgf2 (fgf2 Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Ala 11 mutated to Pro, Ser 55 mutated to Asn

   Promoter

   Available since

   Sept. 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP

   Plasmid

   #158963

   Purpose

   Cre-dependet Cas9-GFP expression

   Depositor

   Michael Brand

   Article

   Kesavan et al Development. 2020 Jun 8;147(11). pii: de

   Inserts

   hsp70l promoter
   floxed Non-FP cassette
   Cas9-GFP
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato

   Plasmid

   #172535

   Purpose

   Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistance

   Depositor

   Richard White

   Article

   Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

   Insert

   -3.5ubb:plin2-tdtomato
   

   Use

   Tags

   Expression

   Mutation

   S80R, A81S, E144D, I148F and R415H mutations obse…

   Promoter

   zebrafish ubb

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR - CaSR-EGFP

   Plasmid

   #191503

   Purpose

   A zebrafish CaSR-EGFP construct used for Gateway cloning.

   Depositor

   Michael Granato

   Article

   Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

   Insert

   CaSR-EGFP-SV40
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. M58 pPET28b-TEV-full-length zebrafish beta catenin

   Plasmid

   #17199

   Depositor

   Randall Moon

   Insert

   beta catenin (ctnnb1 Zebrafish)
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pT3TS-zAsCpf1

   Plasmid

   #107185

   Purpose

   plasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)

   Depositor

   Antonio Giraldez

   Article

   Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

   Insert

   AsCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. mpeg:Rab7-stop-polyA; cmlc2:GFP

    Plasmid

    #213738

    Purpose

    Expresses mCherry-Rab7 in the macrophage lineage of zebrafish with a green heart transgenesis marker

    Depositor

    William Talbot

    Article

    Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

    Insert

    mCherry-Rab7
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    mpeg1.1

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. p5E-pth2

    Plasmid

    #191428

    Purpose

    Tol2 Gateway 5' entry vector containing 2067 bp upstream of the CDS for zebrafish pth2 (ENSDARG00000022951), including the 5' UTR and first intron (GRCz11 chr 17:33493999-33496066)

    Depositor

    Timothy Erickson

    Article

    Venuto et al Biol Open. 2022 Sep 26. pii: 276788. doi

    Insert

    pth2 regulatory region (pth2 Zebrafish)
    

    Use

    Tol2 gateway 5' entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. p5E Lck

    Plasmid

    #135201

    Purpose

    T-cell marker

    Depositor

    David Tobin

    Insert

    Lck promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2-sod1

    Plasmid

    #107594

    Purpose

    Contains zebrafish sod1 gene in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    sod1 (sod1 Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSYC-437

    Plasmid

    #163829

    Purpose

    Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.

    Depositor

    Seok-Yong Choi

    Article

    Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi:

    Inserts

    Foxj1a Promoter
    GCaMP6s
    

    Use

    Multisite gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Danio alpha A-crystallin 1kb promoter/AcGFP

    Plasmid

    #98096

    Purpose

    aA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

    Depositor

    Mason Posner

    Article

    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

    Insert

    Alpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)
    

    Use

    Gfp expressing

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Danio alpha A-crystallin 1 kb fragment (-1028/-1)

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. ZE32 Prickle-1-GFP-pCS2+

    Plasmid

    #17073

    Depositor

    Randall Moon

    Article

    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

    Insert

    Prickle-1 (prickle1a Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

17. MiniCoopR mitfa:KIT L576P

    Plasmid

    #118848

    Purpose

    Expresses human KIT L576P mutant and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    KIT L576P (KIT Human, Zebrafish)
    

    Use

    Zebrafish expression, tol4

    Tags

    Expression

    Mutation

    KIT L576P

    Promoter

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSYC-97

    Plasmid

    #31565

    Depositor

    Seok-Yong Choi

    Article

    Kim et al PLoS One. 2011 . 6(4):e18556.

    Insert

    pCS4+-NLS-EGFP-P2A-mCherry-CAAX
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPRISM-Stop-gcry-mRFP

    Plasmid

    #117772

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    3x3 Stop sequence
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9

    Plasmid

    #118842

    Purpose

    Targets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    cdkn2a gRNA (LOC100329528 Zebrafish)
    

    Use

    CRISPR; Tol2

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge