2,162 results

1. pUFlip-floxed2A-KalTA4-; HE1A:BFP -2

   Plasmid

   #180012

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-KalTA4; HE1A: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-Rab32b

   Plasmid

   #140885

   Purpose

   Expression of zebrafish GFP-tagged Rab32b in mammalian cells.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Rab32b (rab32b Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. flk1 promoter

   Plasmid

   #26436

   Depositor

   Didier Stainier

   Article

   Jin et al Development. 2005 Dec . 132(23):5199-209

   Insert

   flk1 promoter
   

   Use

   Topo cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKHR7

   Plasmid

   #74624

   Purpose

   a cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.

   Depositor

   David Grunwald

   Article

   Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

   Insert

   cmlc2::mCherry
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   cmlc2

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Zebrafish ArcLight Q175

   Plasmid

   #53616

   Purpose

   Genetically encoded voltage sensor ArcLight with improved kinetics

   Depositor

   Vincent Pieribone

   Article

   Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

   Insert

   Zebrafish ArcLight-Q175 (tpte Zebrafish, Aequorea victoria, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Dr-VSD contains R153Q mutation and an amino acid …

   Promoter

   CMV

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. Control-cx43.4-48H-pGTag

   Plasmid

   #117818

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   TagRFP
   

   Use

   Synthetic Biology

   Tags

   CAAX

   Expression

   Mutation

   Promoter

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MiniCoopR mitfa:NRAS

   Plasmid

   #118847

   Purpose

   Expresses human NRAS Q61R mutant and zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   NRAS Q61R (NRAS Zebrafish, Human)
   

   Use

   Zebrafish expression, tol3

   Tags

   Expression

   Mutation

   NRAS Q61R

   Promoter

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tol2-lyzC-mcherry-2a-CDK2

   Plasmid

   #121131

   Purpose

   neutrophil specific expression

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   dre-WT CDK2 (cdk2 Zebrafish)
   

   Use

   Tags

   mcherry-2a

   Expression

   Bacterial

   Mutation

   Promoter

   neutrophil specific lyzC

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3E_he1a:ECFP

   Plasmid

   #113880

   Purpose

   p3E entry vector for Three Multisite Gateway

   Depositor

   Marnie Halpern

   Insert

   he1a (he1.1 Zebrafish)
   

   Use

   Zebrafish danio expression

   Tags

   ECFP

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. hsp70: mKate2-DN Kif5a

    Plasmid

    #105972

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

    Insert

    Kif5a (kif5aa Zebrafish)
    

    Use

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    truncation of aa416-1033

    Promoter

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-PLIN2-tdTOMATO

    Plasmid

    #172533

    Purpose

    PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

    Depositor

    Richard White

    Article

    Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

    Insert

    plin2-tdtomato
    

    Use

    Tags

    Expression

    Mutation

    S80R, A81S, E144D, I148F, and R415H mutations in …

    Promoter

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPRISM-gal4-VP16-gcry-eGFP

    Plasmid

    #117779

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    gal4/VP16
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKHR5

    Plasmid

    #74593

    Purpose

    an alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.

    Depositor

    David Grunwald

    Article

    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

    Insert

    FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
    

    Use

    Cre/Lox; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    alpha-crystallin

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS

    Plasmid

    #78251

    Purpose

    Can be used to subclone in tagged gene of interest into inducible motor neuron expression vector.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Inserts

    -3mnx1 (mnx1 Zebrafish)
    -loxP-mTagBFP2-stop-
    mCherry-NS-MCS
    

    Use

    Zebrafish motor neuron inducible expression

    Tags

    Expression

    Mutation

    Promoter

    -3mnx1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCK069 desmaMCS:minprom_mApple,exorh:EGFP

    Plasmid

    #195982

    Purpose

    Expression construct

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    desmin a regulatory elements, mouse beta-globin minimal promoter mApple, exorh:EGFP (desma Zebrafish)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCK052 p5E exorh:mCherry

    Plasmid

    #195953

    Purpose

    Gateway p5E vector with reporter

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    exorh:mCherry
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. zbFGF

    Plasmid

    #12309

    Depositor

    James Thomson

    Article

    Ludwig et al Nat Methods. 2006 Aug . 3(8):637-646.

    Insert

    Fgf2 (fgf2 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Ala 11 mutated to Pro, Ser 55 mutated to Asn

    Promoter

    Available Since

    Sept. 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. p5'Entry-lck-5.5kb

    Plasmid

    #58890

    Purpose

    provides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenes

    Depositor

    Jeffrey Yoder

    Insert

    zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
    

    Use

    Gateway 5' entry clone

    Tags

    Expression

    Mutation

    mutated single nucleotide (T-->C, 13th nucleot…

    Promoter

    lymphocyte-specific protein tyrosine kinase (lck)…

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. p5E-1.7kbfabp6

    Plasmid

    #159087

    Purpose

    p5E with promoter sequence 1.7kb upstream of zebrafish fabp6 gene

    Depositor

    John Rawls

    Article

    Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

    Insert

    1.7kb fabp6 promoter (fabp6 Zebrafish)
    

    Use

    Promoter fragment 5' entry vector for tol2 c…

    Tags

    Expression

    Mutation

    Promoter

    1.7kb fabp6

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST mfap4:tdTomato

    Plasmid

    #135214

    Purpose

    Red tagged macrophages

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Insert

    mfap4 promoter and tdTomato
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge