2,293 results

1. pLVX-CMV-Galpha-i3 ONE-GO

   Plasmid

   #189737

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-i3 ONE-GO
   GNAI3 (Gnai3 Rat)
   

   Use

   Lentiviral

   Available Since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pEYFP-NR1a

   Plasmid

   #17928

   Depositor

   Stefano Vicini

   Article

   Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

   Insert

   N-methyl-D-aspartate receptor NMDAR1 (Grin1 Rat)
   

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. μ2-HA-WT

   Plasmid

   #32752

   Depositor

   Alexander Sorkin

   Article

   Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

   Insert

   μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCI-SEP GluR2(R)

   Plasmid

   #24001

   Depositor

   Robert Malinow

   Article

   Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

   Insert

   GluR2 (Gria2 Rat)
   

   Tags

   GluR1 signal peptide (aa1-22) and SEP (supereclip…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. LAP2 Full I pAcGFP-N1 monomeric GFP (1317)

   Plasmid

   #62044

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   LAP2beta (Tmpo Rat)
   

   Tags

   AcGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI-EGFP-NR1 wt

   Plasmid

   #45446

   Depositor

   Robert Malinow, Andres Barria

   Article

   Barria et al Neuron. 2002 Jul 18;35(2):345-53.

   Insert

   NR1 (Grin1 Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   EGFP inserted after the predicted signal peptide …

   Promoter

   CMV

   Available Since

   June 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)

   Plasmid

   #127392

   Purpose

   Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)

   Depositor

   Ryohei Yasuda

   Article

   Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   changed Threonine 286 to Aspartic acid and Threon…

   Promoter

   pCAG

   Available Since

   July 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-myc-ERK2-L4A-MEK1_fusion

   Plasmid

   #39197

   Depositor

   Melanie Cobb

   Article

   Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

   Insert

   ERK2-MEK1 Fusion (MAP2K1, Mapk1 Rat, Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   L4A = L33A, L37A, L40A, L42A

   Promoter

   CMV

   Available Since

   July 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV rat SAPAP1

   Plasmid

   #47940

   Purpose

   Expresses rat SAPAP1 in mammalian cells

   Depositor

   Yoshimi Takai, Yutaka Hata

   Article

   Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

   Insert

   SAPAP1 (Dlgap1 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORANGE GFP-Grin2b KI

    Plasmid

    #131487

    Purpose

    Endogenous tagging of GluN2b: N-terminal (amino acid position: S34)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Grin2b Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PSD-95-pTagRFP

    Plasmid

    #52671

    Purpose

    expression of TagRFP-tagged PSD-95

    Depositor

    Johannes Hell

    Insert

    PSD-95 (Dlg4 Rat)
    

    Tags

    tagRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBridge-tyrosinase.σ2

    Plasmid

    #197415

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-2 σ2
    

    Tags

    GAL4-DNA binding domain fragment, HA tag, and nuc…

    Expression

    Yeast

    Mutation

    encodes R42G substitution, contains silent substi…

    Promoter

    ADH1 and MET25

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.hSyn.FLEX.iGluSnFR3.v82.GPI.codonopt

    Plasmid

    #175184

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 82 in GPI backbone. iGluSnFR3.v82.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn FLEX iGluSnFR3 v82.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Promoter

    human Synapsin

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-CMV-Galpha-z ONE-GO

    Plasmid

    #189734

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-z ONE-GO
    GNAZ (Gnaz Rat)
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. Lenti-evoA1-BE4max

    Plasmid

    #174700

    Purpose

    Base Editing

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

    Insert

    Lenti-evoA1-BE4max
    

    Use

    Lentiviral

    Promoter

    EFS

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV Myc rat SAPAP4

    Plasmid

    #40218

    Depositor

    Yoshimi Takai, Yutaka Hata

    Article

    Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

    Insert

    SAPAP4 (Dlgap4 Rat)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    N834D

    Promoter

    CMV

    Available Since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pCMV Myc rat SAPAP2

    Plasmid

    #40216

    Depositor

    Yoshimi Takai, Yutaka Hata

    Article

    Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

    Insert

    SAPAP2 (Dlgap2 Rat)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    H137Q and K806R

    Promoter

    CMV

    Available Since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

18. AP2u2-mCherry

    Plasmid

    #27672

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Ap2m1 (Ap2m1 Rat)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-hSyn-RiboL1-jGCaMP8m

    Plasmid

    #169248

    Purpose

    AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8m protein.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    RiboL1-jGCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-His6-ERK2-MEK1_R4F_coexpression

    Plasmid

    #39212

    Depositor

    Melanie Cobb

    Article

    Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

    Inserts

    Mek1 R4F (MAP2K1 Human)
    Erk2 (Mapk1 Rat)
    

    Tags

    His6 and RBS

    Expression

    Bacterial

    Mutation

    MEK1(R4F) = S218E, S222D and deletion of AA32-51

    Promoter

    T7 and T7 via RBS

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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