We narrowed to 2,010 results for: ARP

1. msPlekha3 (msFapp1) g2 lentiCRISPRv2-opti

   Plasmid

   #218649

   Purpose

   Knockout vector for mouse Plekha3 (Fapp1)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   Plekha3 (Plekha3 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. msPlekha3 (msFapp1) g1 lentiCRISPRv2-opti

   Plasmid

   #218648

   Purpose

   Knockout vector for mouse Plekha3 (Fapp1)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   Plekha3 (Plekha3 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. msPlekha8 (msFapp2) g2 lentiCRISPRv2-opti

   Plasmid

   #218651

   Purpose

   Knockout vector for mouse Plekha8 (Fapp2)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   Plekha8 (Plekha8 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PLEKHA3 (FAPP1) g2 lentiCRISPRv2-opti

   Plasmid

   #218657

   Purpose

   Knockout vector for human PLEKHA3 (FAPP1)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   PLEKHA3 (PLEKHA3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PLEKHA8 (FAPP2) g1 lentiCRISPRv2-opti

   Plasmid

   #218658

   Purpose

   Knockout vector for human PLEKHA8 (FAPP2)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   PLEKHA8 (PLEKHA8 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PLEKHA3 (FAPP1) g1 lentiCRISPRv2-opti

   Plasmid

   #218656

   Purpose

   Knockout vector for human PLEKHA3 (FAPP1)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   PLEKHA3 (PLEKHA3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPLS24

   Plasmid

   #17285

   Depositor

   Nancy Craig

   Article

   Sharpe et al EMBO J. 1998 Oct 1. 17(19):5822-31.

   Insert

   TnsD (tnsD E. coli transposon Tn7)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pLenti UBC LysoFlux (HaloTag7-mGFP-Galectin3)

   Plasmid

   #215008

   Purpose

   Express a Lysophagy reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Flag-SRm160

   Plasmid

   #11305

   Depositor

   Phil Sharp

   Article

   Cheng et al Mol Cell Biol. 2006 Jan . 26(1):362-70.

   Insert

   SRm160 (SRRM1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE pGK LC3Flux (HaloTag7-mGFP-LC3-STOP)

    Plasmid

    #215015

    Purpose

    Express an autophagy (LC3) reporter in mammalian cells (untested)

    Depositor

    Wade Harper

    Insert

    MAP1LC3B (Map1lc3b Rat)
    

    Use

    Lentiviral

    Tags

    HaloTag(7)-mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDEST17-UbE2D3

    Plasmid

    #15784

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2D3 (UBE2D3 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    see Depositor Comments below

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTwist-msEEA1

    Plasmid

    #214991

    Purpose

    Gateway entry vector with mouse EEA1

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (Eea1 Mouse)
    

    Use

    Gateway

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDEST17-UbE2R2

    Plasmid

    #15806

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2R2 (UBE2R2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)

    Plasmid

    #215014

    Purpose

    Express an autophagy (LC3) reporter in mammalian cells (untested)

    Depositor

    Wade Harper

    Insert

    MAP1LC3B (Map1lc3b Rat)
    

    Use

    Lentiviral

    Tags

    HaloTag(7)-mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCVD468

    Plasmid

    #66155

    Purpose

    expression of His-tagged espA

    Depositor

    James Kaper

    Article

    Karpman et al Pediatr Nephrol. 2002 Mar;17(3):201-11.

    Insert

    espA
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCVD469

    Plasmid

    #66156

    Purpose

    expression of His-tagged espB

    Depositor

    James Kaper

    Article

    Karpman et al Pediatr Nephrol. 2002 Mar;17(3):201-11.

    Insert

    espB
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pXDC61-LepA

    Plasmid

    #21843

    Depositor

    Howard Shuman

    Article

    Charpentier et al PLoS Pathog. 2009 Jul . 5(7):e1000501.

    Insert

    LepA (lepA L. pneumophila)
    

    Use

    Tags

    TEM

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Rab11A[8-175]-His-C Q70L

    Plasmid

    #228042

    Purpose

    Protein purification construct. Rab11 crystallography truncation with a C-terminal cys for maleimide labeling. Constitutively active mutation (Q70L).

    Depositor

    Wade Harper

    Insert

    Rab11A[8-175]-His-C Q70L (RAB11A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q70L

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE pGK LysoFlux (HaloTag7-mGFP-Galectin3)

    Plasmid

    #215009

    Purpose

    Express a Lysophagy reporter in mammalian cells (untested)

    Depositor

    Wade Harper

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Lentiviral

    Tags

    HaloTag(7)-mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV UBC hTMEM115-3C-3xHA-P2A-eGFP

    Plasmid

    #214996

    Purpose

    Stably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    TMEM115 (TMEM115 Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET15-UbE2D1

    Plasmid

    #15782

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
    

    Use

    Tags

    6x His, T7 tag, and Xpress tag (enterokinase clea…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDEST17-Cdc34a

    Plasmid

    #18674

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Cdc34A (CDC34 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

23. pEC2483 (pSpy1C)

    Plasmid

    #220248

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDA303

    Plasmid

    #195199

    Purpose

    Overexpressing 6His-TetR in E. coli.

    Depositor

    Rahul Sarpeshkar

    Article

    Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

    Insert

    TetR
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T5 promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. HC-M

    Plasmid

    #173481

    Purpose

    An rrnB P1-based GFP ATP reporter in E. coli

    Depositor

    Rahul Sarpeshkar

    Article

    Deng et al BMC Biol. 2021 May 17;19(1):101. doi: 10

    Insert

    GFP-mut2
    

    Use

    Synthetic Biology

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    rrnB P1

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV UBC msTMEM115-3C-3xHA-P2A-eGFP

    Plasmid

    #214997

    Purpose

    Stably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    TMEM115 (Tmem115 Mouse)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MSCV puro let-7 sponge

    Plasmid

    #29766

    Depositor

    Phil Sharp

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

    Insert

    let-7 sponge
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2011

    Availability

    Academic Institutions and Nonprofits only

28. pLV UBC msNLRP3(WT)-APEX2-3xFLAG-IRES-eGFP

    Plasmid

    #218633

    Purpose

    Proximity biotinylation of msNLRP3

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Lentiviral

    Tags

    APEX2-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV UBC APEX2-3xFLAG-IRES-eGFP

    Plasmid

    #214983

    Purpose

    Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    APEX2 (APX2 Glycine max (soybean))
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    UBC

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MSCV-N-Flag-HA-GFP

    Plasmid

    #41034

    Purpose

    Retroviral expression of GFP in mammalian cells

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    GFP
    

    Use

    Retroviral; Gateway destination vector

    Tags

    Flag, HA, and IRES Puro

    Expression

    Mammalian

    Mutation

    Promoter

    LTR-driven expression

    Available since

    Oct. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

31. p4580 AU-IRF-3a

    Plasmid

    #8660

    Depositor

    Peter Howley

    Article

    Karpova et al Mol Cell Biol 2001 Jul;21(13):4169-76.

    Insert

    IRF-3a (IRF3 Human)
    

    Use

    Tags

    AU

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2005

    Availability

    Academic Institutions and Nonprofits only

32. msHook2 g1 lentiCRISPRv2-mCherry

    Plasmid

    #218652

    Purpose

    Knockout vector for mouse Hook2

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    Hook2 (Hook2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDONR221 msNLRP3 (STOP)

    Plasmid

    #218638

    Purpose

    Gateway shuttling vector for cloning N-terminal NLRP3 tags (contains a stop codon--note: bulky N-terminal tags are likely not functional)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. msHook2 g2 lentiCRISPRv2-mCherry

    Plasmid

    #218653

    Purpose

    Knockout vector for mouse Hook2

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    Hook2 (Hook2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFOSGE_COF2A07

    Plasmid

    #70902

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Arp5 (Arp5 Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

36. pEC3135 (pERASE)

    Plasmid

    #220306

    Purpose

    Enables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pPB_TRE3G_scFV-sfGFP

    Plasmid

    #235573

    Purpose

    Dox-inducible expression of control superfolder (sf)GFP fused with scFV

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    superfolder (sf)fGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPB_TRE3G_dCas9-5xGCN4_Hygro

    Plasmid

    #235572

    Purpose

    Dox-inducible expression of dCas9-GCN4 & genomic integration

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    dCas9-5xGCN4
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A; H840A

    Promoter

    Available since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-DN-hCUL1-FLAG

    Plasmid

    #15818

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin1 (CUL1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N452

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-DN-hCUL4A-FLAG

    Plasmid

    #15821

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin4A (CUL4A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N440

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3-DN-hCUL5-FLAG

    Plasmid

    #15823

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin5 (CUL5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N441

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3-DN-hCUL2-FLAG

    Plasmid

    #15819

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin2 (CUL2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N427

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pTargeT gE

    Plasmid

    #60845

    Purpose

    Expression of the varicella zoster glycoprotein (gE) in mammalian cells

    Depositor

    Charles Grose

    Article

    Carpenter et al J Virol. 2011 Sep;85(18):9414-24. doi: 1

    Insert

    ORF68 (ORF68 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

44. pHAGE-FLAG-APEX2-GFP

    Plasmid

    #175757

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GFP (Control for APEX experiments)

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pEC2735 (pSpy0C2)

    Plasmid

    #220279

    Purpose

    integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. hTMEM115-TEV-3xHA-P2A-mScarlet HDR template

    Plasmid

    #214998

    Purpose

    Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    hTMEM115-TEV-3xHA-P2A-mScarlet HDR template (TMEM115 Human)
    

    Use

    Hdr template

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    None

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template

    Plasmid

    #214999

    Purpose

    Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mNG for cell sorting.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template (TMEM115 Human)
    

    Use

    Hdr template

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    None

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pHAGE pGK hNLRP3-mNG

    Plasmid

    #218643

    Purpose

    Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak pGK promoter with blast selection

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pGEX-2TK-PTB

    Plasmid

    #21929

    Depositor

    Phil Sharp

    Article

    Garcia-Blanco et al Genes Dev. 1989 Dec . 3(12A):1874-86.

    Insert

    PTB (PTBP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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50. pDONR221 hNLRP3-mNG-STOP

    Plasmid

    #218644

    Purpose

    Gateway vector for shuttling NLRP3 C-terminally tagged with mNeonGreen to different destination vectors (start and stop codon)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Gateway entry

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge