We narrowed to 818 results for: VILL;

1. pLV-EF1A-LivePAR-Hygro

   Plasmid

   #176063

   Purpose

   EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   LivePAR
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMJ179

   Plasmid

   #85996

   Purpose

   One-guide Perturb-seq vector backbone; modified mouse U6 promoter; constant region 2

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   EGFP-NT2_cr2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   modified mouse U6

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-BAIAP2 wt

   Plasmid

   #31675

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   BAI1-associated protein 2 (BAIAP2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLV-Bsd-CMV-hUCK

   Plasmid

   #195141

   Purpose

   UCK2 overexpression vector with blasticidin resistance

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   UCK2 (UCK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pW34

   Plasmid

   #158210

   Purpose

   LuxCDABE operon (R6K ori) under T7, in loxP and lox5171 sites.

   Depositor

   Yasuo Yoshikuni

   Article

   Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

   Insert

   LuxABCDE
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV-Hygro-EF1A-OB-Linker-eGFP

   Plasmid

   #176068

   Purpose

   EGFP fused to the C-terminus of an OB domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   OB
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLV-Hygro-EF1A-FHA-Linker-eGFP

   Plasmid

   #176065

   Purpose

   EGFP fused to the C-terminus of a FHA domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   FHA
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Chr1q_Centromere-Targeting_gRNA

   Plasmid

   #195125

   Purpose

   gRNA targeting centromere-proximal location on Chromosome 1q in a third generation Cas9 backbone with GFP

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   Chr1q gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV-Hygro-EF1A-Macro-Linker-eGFP

   Plasmid

   #176067

   Purpose

   EGFP fused to the C-terminus of a Macrodomain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   Macro
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT7-7 aSyn C141

    Plasmid

    #108866

    Purpose

    Expresses human WT alpha synuclein with a C-terminal cysteine

    Depositor

    Gabriele Kaminski Schierle

    Article

    Stephens et al bioRxiv 2022.10.04.510646

    Insert

    alpha synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C141 mutation to allow C-terminal maleimide dye l…

    Promoter

    T7

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV-Hygro-EF1A-WWE-Linker-eGFP

    Plasmid

    #176072

    Purpose

    EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    WWE
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV-Hygro-EF1A-PBZC-Linker-eGFP

    Plasmid

    #176070

    Purpose

    EGFP fused to the C-terminus of a PBZ domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PBZ-C
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV-Hygro-EF1A-PBM-Linker-eGFP

    Plasmid

    #176069

    Purpose

    EGFP fused to the C-terminus of a PBM domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PBM
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pW5Y_Apr

    Plasmid

    #158211

    Purpose

    BAC-based, lacUV5 regulon and Cre recombinase in loxP and lox5171 sites

    Depositor

    Yasuo Yoshikuni

    Article

    Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV-Hygro-EF1A-LivePARBackbone

    Plasmid

    #176526

    Purpose

    Expression vector with a BamHI site in-frame with a Gly-Ser linker fused to EGFP; serves as the backbone for PAR binding domain incorporation for LivePAR

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Rab8WT(Flag) in pcDNA3.1neo

    Plasmid

    #46783

    Purpose

    expression of WT Rab8a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB8A (RAB8A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

17. Chr7_Centromere-Targeting_gRNA

    Plasmid

    #195129

    Purpose

    dual gRNA vector targeting centromere-proximal locations on Chromosome 7p and 7q in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Chr7 gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MSCV-Neo-GFP/empty

    Plasmid

    #105505

    Purpose

    Empty backbone of MSCV-driven retroviral cDNA expression

    Depositor

    Christopher Vakoc

    Article

    Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. h-Plekhm1_FLAG-pcDNA 3.1

    Plasmid

    #73593

    Purpose

    Express human Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAVS1_gRNA

    Plasmid

    #196139

    Purpose

    gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    AAVS1 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Chr8q_Centromere-Targeting_gRNA

    Plasmid

    #195128

    Purpose

    gRNA targeting centromere-proximal location on Chromosome 8q in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Chr8q gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PCMV-intron myc Rab11WT

    Plasmid

    #46785

    Purpose

    expression of WT Rab11a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB11A (RAB11A Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

23. Rosa26_gRNA

    Plasmid

    #196138

    Purpose

    gRNA targeting the Rosa26 locus in a thid generation Cas9 backbone with TagBFP2

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Rosa26 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pECE-M2-GPHN

    Plasmid

    #31665

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    Gephyrin (GPHN Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pECE-M2-PPP1R12C wt

    Plasmid

    #31659

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    Protein Phosphatase 1 Regulatory Subunit 12C (PPP1R12C Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    codon-optimized

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

26. EGFP-Rab7a-7

    Plasmid

    #56443

    Purpose

    Localization: Ras GTPase, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

    Insert

    Rab7a (RAB7A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N117S in NM_004637.5

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

27. 783 pCScherryActMEK

    Plasmid

    #31880

    Depositor

    Nathan Lawson

    Article

    Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Expression vector

    Tags

    mCherry

    Expression

    Mutation

    Ser218Glu Ser222Asp; activated MEKK

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

28. pDEST_6xUAS:Lamp1-mGFP

    Plasmid

    #192356

    Purpose

    Lamp1 tagged with mGFP, expressed under the UAS promoter

    Depositor

    Francesca Peri

    Article

    Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

    Insert

    Lamp1 (LAMP1 Human)
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GFP-P2A-CIAR_pLenti_CMV_Puro

    Plasmid

    #86503

    Purpose

    Lenti plasmid for generating CIAR expressing stable cell lines

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    GFP-P2A-CIAR (SOS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T968L in SOScat

    Promoter

    CMV

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. OR6K6_Deletion_gRNA2

    Plasmid

    #195196

    Purpose

    dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    OR6K6 dual gRNA (OR6K6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. OR6K6_Deletion_gRNA1

    Plasmid

    #195195

    Purpose

    dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    OR6K6 dual gRNA (OR6K6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. OR6K6_Deletion_gRNA3

    Plasmid

    #195197

    Purpose

    dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    OR6K6 dual gRNA (OR6K6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pT2/GD-IRES-GFP-CTNNB1

    Plasmid

    #192868

    Purpose

    Carries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.

    Depositor

    David Largaespada

    Article

    Keng et al Hepatology. 2013 Jan;57(1):120-30. doi:

    Inserts

    luc+
    EGFP
    CTNNB1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

    Plasmid

    #188541

    Purpose

    Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

    Depositor

    Darrell Kotton

    Article

    Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti-CMV-DNAJC5 (L115R)

    Plasmid

    #205728

    Purpose

    Mammalian expression of DNAJC5 (L115R)

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (L115R) (DNAJC5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    L115R

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. hPlekhm1-dsRedM

    Plasmid

    #73592

    Purpose

    Express human Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    dsRed

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

37. CIAR_pcDNA5/FRT/TO

    Plasmid

    #86502

    Purpose

    Expresses CIAR in mammalian cells. Used to generate Flp-In stables.

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CIAR (SOS1 Human)
    

    Use

    Frt

    Tags

    Expression

    Mammalian

    Mutation

    T968L in SOScat

    Promoter

    CMV (tet operator)

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LentiV_Neo_SIK3_CR_T221A

    Plasmid

    #108107

    Purpose

    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TA mutation at LKB1 phosphorylation site) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    SIK3 (SIK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    change guanine 501 to cytosine (silent mutation),…

    Promoter

    EFS promoter

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGEX-4T-3-TRC8 513-664

    Plasmid

    #185352

    Purpose

    RING domain-containing GST fusion protein for ubiquitination assays.

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    TRC8 (RNF139 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 513-664 only

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET3a-UBE2J1 1-283

    Plasmid

    #185348

    Purpose

    In vitro transcription and translation in bacterial system

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    UBE2J1 (UBE2J1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cytoplasmic domain only, aa 1-283

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pGL-p21UTRm1

    Plasmid

    #20878

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p21 3'UTR site 1 mutation (Cdkn1a Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Predicted miRNA binding site 1 (Position ~420) GC…

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

42. pLV-CMV-EGFP-PolB-Hygro

    Plasmid

    #176056

    Purpose

    EGFP fused to the N-terminus of PolB & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. LRG-2.1T-neo-sgTAF12(human)#4.4

    Plasmid

    #105590

    Purpose

    lentivirally express gRNA targeting human TAF12 HFD with GFP marker and neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    gRNA targeting human TAF12 HFD
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pGL-p21UTRm2

    Plasmid

    #20879

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p21 3'UTR site 2 mutation (Cdkn1a Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Predicted miRNA binding site 2 (Position ~1100) G…

    Promoter

    Available since

    March 12, 2012

    Availability

    Academic Institutions and Nonprofits only

45. LentiV_Neo_SIK3_CR_T142Q

    Plasmid

    #108109

    Purpose

    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & gatekeeper mutation) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    SIK3 (SIK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    change guanine 501 to cytosine (silent mutation),…

    Promoter

    EFS promoter

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. 6xHis-TEV-GEI-17(133-509)

    Plasmid

    #87056

    Purpose

    Expresses 6xHis-tagged gei-17 (isoform f), with a TEV protease cleavage site between the tag and the ORF

    Depositor

    Ronald Hay

    Article

    Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

    Insert

    GEI-17 isfoomr f (aa 133-509) (gei-17 Nematode)
    

    Use

    Tags

    6xHis-TEV and GAGGGS linker

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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47. MSCV-PIG-FLAG-TAF4(827-909)

    Plasmid

    #105617

    Purpose

    retrovirally express mouse TAF4 HFD with 3*FLAG at N terminal, GFP marker and puro resistance,

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    FLAG tagged TAF4 HFD-827-909 (Taf4 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTR

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. p2701-AGER-tdTomato

    Plasmid

    #216470

    Purpose

    donor vector for targeting a tdTomato reporter to the human AGER locus at the endogenous ATG start site

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Insert

    tdTomato
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. p2707-pHAGE-Ef1aL-dsRed-UBC-tagBFP

    Plasmid

    #216476

    Purpose

    lentiviral dual expression of dsRed and tagBFP

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    dsRed
    tagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1aL and UBC

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCMV-DNAJC5-ΔC30 (Δ169-198)

    Plasmid

    #205722

    Purpose

    Mammalian expression of DNAJC5-ΔC30 (Δ169-198)

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ169-198

    Promoter

    Available since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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