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Showing: 501 - 550 of 638 results

  15. pDUAL_2_lacZ

    Plasmid
    #155173
    Purpose
    see pDUAL_lacZ: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    155173-plasmid-map-sequence-id-310557

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  16. pDUAL_2_tacI

    Plasmid
    #155174
    Purpose
    see pDUAL_tacI: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    155174-plasmid-map-sequence-id-310560

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  17. pBICI_2_medium

    Plasmid
    #155167
    Purpose
    see pBICI_medium: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    155167-plasmid-map-sequence-id-310552

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  18. pAL928

    Plasmid
    #119853
    Purpose
    precusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427C
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Cysteine 169 to Serine, Cysteine 179 to Serine, C…
    Promoter
    T7
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119853-plasmid-map-sequence-id-232052

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  24. LZ10 PBREBAC-H2BHalo

    Plasmid
    #91564
    Purpose
    piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.
    Depositor
    James Zhe Liu
    Article
    Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1
    Insert
    histone H2B-HaloTag
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    91564-plasmid-map-sequence-id-180643

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  27. pEND-351_Cacnes_dCas9_CRISPRi

    Plasmid
    #225617
    Purpose
    Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225617-plasmid-map-sequence-id-451418

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  29. pAM4787

    Plasmid
    #120088
    Purpose
    Cyanobacteria synthetic plasmid to test the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS).
    Depositor
    Susan Golden
    Article
    Chen et al Microbiology. 2016 Dec;162(12):2029-2041
    Insert
    Plasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120088-plasmid-map-sequence-id-234398

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  30. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

    Plasmid
    #199252
    Purpose
    Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Insert
    High specificity eSpCas9(1.1)-D10A nickase
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A K848A K1003A R1060A
    Promoter
    CAG promoter
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199252-plasmid-map-sequence-id-391383

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  35. pPTG-GG-sg2+sg3

    Plasmid
    #127199
    Purpose
    Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter
    Depositor
    Jordan Green
    Article
    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6
    Insert
    tRNA-gRNA
    Use
    Empty sgrna plasmid
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    127199-plasmid-map-sequence-id-248025

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  36. pJN601

    Plasmid
    #59790
    Purpose
    zf1::yfp fusion with floxed unc-119(+) within an intron of yfp. For creating zf1::yfp knock-ins using unc-119 as a marker
    Depositor
    Jeremy Nance
    Article
    Armenti et al Development. 2014 Dec;141(23):4640-7. do
    Inserts
    ZF1 domain from pie-1
    N-terminal half of yfp with introns
    intron containing floxed unc-119 gene in reverse orientation
    C-terminal half of yfp with introns
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59790-plasmid-map-sequence-id-97553

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  43. AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA

    Plasmid
    #199254
    Purpose
    Expression construct encoding the high specificity SpCas9-KARA-D10A nickase
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Insert
    High specificity S. pyogenes Cas9-D10A-KARA nickase
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A K848A R1060A
    Promoter
    CAG promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199254-plasmid-map-sequence-id-391656

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Showing: 501 - 550 of 638 results