We narrowed to 1,640 results for: plasmid for e coli
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Plasmid#112599PurposeUsed for expression of protein in E. coli as N-terminal Sumo tag fusionsDepositorTypeEmpty backboneUseTagsSumoExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only
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pCRISPathBrick
Plasmid#65006PurposeE. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricksDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive native promotersAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc Dam_f_GFPoNLS
Plasmid#85820PurposeiDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc Dam_f_GFPoNLS
UseTagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A.PromoterAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSPB845
Plasmid#128296Purposeasymmetric plasmid partitioning E. coli - regulatory plasmidDepositorInsertPLlacO1 - lp-cfp-parB, BBa_J23110 - lacI
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAA41
Plasmid#128281Purposeasymmetric plasmid partitioning E. coli - target plasmidDepositorInsertPiq - mRFP, parS (3.2 kb spacer between parS and Piq)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCC48
Plasmid#128285Purposeasymmetric plasmid partitioning E. coli - target plasmidDepositorInsertPiq - mRFP, BBa_J23100 - phlF, parS
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDOC-GG
Plasmid#149377PurposeChassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene DoctoringDepositorInsertsFragment containing a kanamycin resistance cassette
Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
Fragment containing an I-SceI recognition sequence
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpot2
Plasmid#117063PurposeFor expression of proteins with C-terminal Spot-Tag in E. coliDepositorTypeEmpty backboneUseTagsSpot-TagExpressionBacterialMutationPromoterAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBXNPHM3-Nb_MsbA#1
Plasmid#186428PurposePlasmid for bacterial expression of MsbA binding Nanobody Nb_MsbA#1DepositorInsertNanobody Nb_MsbA#1
UseAffinity Reagent/ AntibodyTags3C cleavage site, His Tag, Maltose Binding Protei…ExpressionMutationPromoterpBADAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOP2H
Plasmid#112596PurposeUsed for expression of protein in E. coli as non-cleavable C-terminal 8His-tag fusionsDepositorTypeEmpty backboneUseTags8HisExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTar-ipgC
Plasmid#97097PurposeExpresses ipgC in the presence of E. coli sigma factor ?28DepositorInsertIpgC
UseTagsExpressionBacterialMutationPromoterpTarAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPR219.05
Plasmid#78554PurposePlasmid expressing UirR, response regulator and PcsiR1-regulated GFP outputDepositorInsertuirR
UseSynthetic BiologyTagsExpressionMutationPromoterJ23115Available sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDSG589
Plasmid#214217Purposecompetition integrase RBS library parent plasmid (myc-tagged)DepositorInsertPhlF, A118
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSG588
Plasmid#214216Purposebiphasic target plasmid version 2.0 ("flip-proof")DepositorInsertfolA, trpC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
mUAVbla:tetR 5′
Plasmid#149379PurposemUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangsDepositorInserttetA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-rfp
Plasmid#153038PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.DepositorInsertAscpf1
UseTagsExpressionBacterialMutationE993APromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-Δ
Plasmid#153039PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.DepositorInsertAscpf1
UseTagsExpressionBacterialMutationE993APromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
p005kanRFP
Plasmid#58533PurposeRFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant.DepositorInsertRed fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT5 between two lac operatorsAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWaaD
Plasmid#210448PurposeExpress waaD under the control of PBADDepositorInsertwaaD
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaID
Plasmid#210449PurposeExpress waaI and waaD under the control of PBADDepositorInsertwaaI and waaD
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaJ
Plasmid#210450PurposeExpress waaJ under the control of PBADDepositorInsertwaaJ
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaK
Plasmid#210451PurposeExpress waaK under the control of PBADDepositorInsertwaaK
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaRK
Plasmid#210452PurposeExpress waaR and waaK under the control of PBADDepositorInsertwaaR and waaK
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaT
Plasmid#210453PurposeExpress waaT under the control of PBADDepositorInsertwaaT
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaW
Plasmid#210454PurposeExpress waaW under the control of PBADDepositorInsertwaaW
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaV
Plasmid#210457PurposeExpress waaV under the control of PBADDepositorInsertwaaV
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaVL
Plasmid#210458PurposeExpress waaV and waaL under the control of PBADDepositorInsertwaaV and waaL
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaaX
Plasmid#210459PurposeExpress waaX under the control of PBADDepositorInsertwaaX
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_5IHW
Plasmid#234148PurposeHeterologous protein expression of serine-aspartate repeat-containing protein E in Escherichia coliDepositorInsert5IHW
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDest-557
Plasmid#161890PurposeStandard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions in E. coliDepositorTypeEmpty backboneUseTagsMBPExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOP2S
Plasmid#112597PurposeUsed for expression of protein in E. coli as non-cleavable C-terminal Strep Tag fusionsDepositorTypeEmpty backboneUseTagsStrep tagExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTar-deGFP
Plasmid#97098PurposeExpresses GFP in the presence of E. coli sigma factor ?28DepositorInsertdeGFP
UseTagsExpressionBacterialMutationPromoterpTarAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ2-Pdadh-LacZ
Plasmid#191618PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ8-CadR-Pcadh-LacZ
Plasmid#191623PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ with transcriptional regulator CadRDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ8.1-Pcadh-LacZ
Plasmid#191624PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ9-Phcdh-LacZ
Plasmid#191625PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Phcdh hydrocaffeic acid dehydroxylase promoter-lacZDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6409
Plasmid#47048PurposeBglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPlacUV5Available sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6410
Plasmid#47049PurposeBglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPlacUV5Available sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAT-9208
Plasmid#124221PurposeCloning plasmid for a self-targeting gRNA libraryDepositorInsertself-targeting gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterJ23119Available sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBA337
Plasmid#214750PurposeMedium copy thyA-infA plasmid containing bicistronic gfp-specR for use in mouse work validationDepositorInsertBBa_J23119-RiboJ-RBS(21992)-gfpmut3-RBS(4533)-specR
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEINO1
Plasmid#179472PurposeMulti-host shuttle plasmid for D. radiodurans, E. coli, S. cerevisiae, S. meliloti, and P. tricornutum; contains an oriT for transfer via conjugationDepositorInsertsori
cat
UseSynthetic Biology; Bacterial and yeast cloningTagsExpressionMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ oDam_f_GFPoNLS
Plasmid#85819PurposeiDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertoDam_f_GFPoNLS
UseTagsoNLS (optimized nuclear localization signal) C te…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…PromoterAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSIJ8
Plasmid#68122PurposeTemperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expressionDepositorInsertsflp recombinase
rhaS
rhaR
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceDec. 8, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
BirA_BVES untagged
Plasmid#196581Purposeexpresses BirADepositorInsertBirA_Ecoli
UseBaculovirus expressionTagsExpressionMutationPromoterAvailable sinceJune 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOP3MT
Plasmid#112601PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and MBP fusionsDepositorTypeEmpty backboneUseTagsAvi - 8His-MBPExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP2HA
Plasmid#112598PurposeUsed for expression of protein in E. coli as non-cleavable C-terminal 8His-Avi tag fusionsDepositorTypeEmpty backboneUseTags8His-Avi tagExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP3GT
Plasmid#112607PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and GST fusionsDepositorTypeEmpty backboneUseTagsAvi - 8His-GSTExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP3BT
Plasmid#112603PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and GB1 fusionsDepositorTypeEmpty backboneUseTagsAvi - 8His-GB1ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP3MP
Plasmid#112600PurposeUsed for expression of protein in E. coli as PreScission cleavable N-terminal Avi-His8-tag and MBP fusionsDepositorTypeEmpty backboneUseTagsAvi - 8His-MBPExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP3TP
Plasmid#112604PurposeUsed for expression of protein in E. coli as PreScission cleavable N-terminal Avi-His8-tag and thioredoxin fusionsDepositorTypeEmpty backboneUseTagsAvi - 8His-thioredoxinExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only