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We narrowed to 9,306 results for: try

Showing: 501 - 550 of 9306 results

  32. pTrex-n-eGFP

    Plasmid
    #62544
    Purpose
    Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome
    Depositor
    Rick Tarleton
    Article
    Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.
    Insert
    eGFP
    Use
    (1)trypanosoma cruzi expression of egfp (2) genom…
    Tags
    None
    Expression
    Mutation
    None
    Promoter
    Ribo-HX1
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62544-plasmid-map-sequence-id-109759

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  33. pYZ033

    Plasmid
    #98404
    Purpose
    Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1
    Depositor
    Jef Boeke
    Article
    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    98404-plasmid-map-sequence-id-198860

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  37. pRPa-iSL(MCS1+2)

    Plasmid
    #69244
    Purpose
    Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.
    Depositor
    David Horn
    Article
    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7
    Type
    Empty backbone
    Use
    Expression in trypanosoma brucei
    Tags
    Expression
    Mutation
    Promoter
    RRNA promoter
    Available since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69244-plasmid-map-sequence-id-124145

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  41. WasCFP /pQE-30

    Plasmid
    #79858
    Purpose
    Cyan-green fluorescent protein with tryptophan-based chromophore in equilibrium between anionic and neutral states.
    Depositor
    Konstantin Lukyanov
    Article
    Sarkisyan et al Sci Rep. 2012;2:608. doi: 10.1038/srep00
    Insert
    WasCFP
    Use
    Tags
    HisTag
    Expression
    Bacterial
    Mutation
    Cyan fluorescent protein mCerulean with substitut…
    Promoter
    T5 promoter / lac operator
    Available since
    Sept. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79858-plasmid-map-sequence-id-160287

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  42. pET11a-DAAT-V33G/T242G

    Plasmid
    #159278
    Purpose
    Aminotransferase variant with near-native catalytic efficiency toward D-tryptophan
    Depositor
    Roberto Chica
    Article
    Parmeggiani et al ACS Catalysis 9, 3482–3486.
    Insert
    Engineered d-alanine aminotransferase
    Use
    Tags
    6x His tag
    Expression
    Bacterial
    Mutation
    Valine 33 and threonine 242 have been mutated to …
    Promoter
    T7
    Available since
    Sept. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    159278-plasmid-map-sequence-id-311390

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  46. pPOTv7-puro-OsAID (p5109)

    Plasmid
    #239359
    Purpose
    pPOTv7 based plasmid template for addition of a Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin
    Depositor
    Mark Carrington
    Article
    Alexandra et al bioRxiv 2025.06.17.660065
    Insert
    Rice Auxin Inducible Degradation domain
    Use
    Bacterial
    Tags
    Expression
    Mutation
    Promoter
    none
    Available since
    July 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    239359-plasmid-map-sequence-id-478812

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  48. BPTI-21

    Plasmid
    #195058
    Purpose
    simplyfied amino acid sequence by alanine scanning
    Depositor
    Yutaka Kuroda, Peter Kim
    Article
    Kuroda et al J Mol Biol. 2000 May 5;298(3):493-501. d
    Insert
    bovine pancreatic trypsin inhibitor
    Use
    Tags
    His tag and trpΔLE leader sequences
    Expression
    Bacterial
    Mutation
    21 amino acids are Alanine out of 58 amino acids
    Promoter
    T7 promotor
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195058-plasmid-map-sequence-id-388061

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Showing: 501 - 550 of 9306 results