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We narrowed to 14,223 results for: Cas9

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Showing: 201 - 220 of 14223 results

  3. pHSN6I01

    Plasmid
    #50587
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-KRAB
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 that is defective in DNA cleavage; the maiz…
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50587-plasmid-map-sequence-id-75659

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  4. PX408 Francisella tularensis subsp. novicida Cas9

    Plasmid
    #68705
    Purpose
    Francisella tularensis subsp. novicida Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FtCas9
    Use
    CRISPR
    Tags
    HA-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68705-plasmid-map-sequence-id-140302

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  6. pRCC-N

    Plasmid
    #81192
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Publication
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81192-plasmid-map-sequence-id-158700

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  10. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

    Plasmid
    #64219
    Purpose
    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A
    Depositor
    Ralf Kuehn
    Publication
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sgRNA targeting ROSA26-1
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-BFP-P2A-E1B
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    May 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64219-plasmid-map-sequence-id-107960

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  11. pCMV-PE2

    Plasmid
    #132775
    Purpose
    Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b
    Depositor
    David Liu
    Publication
    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    See manuscript
    Promoter
    CMV
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    132775-plasmid-map-sequence-id-259720

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  14. pSpCas9n(BB)-2A-Puro (PX462) V2.0

    Plasmid
    #62987
    Purpose
    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)
    Depositor
    Feng Zhang
    Publication
    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9n-2A-Puro
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Mutation
    Cas9 D10A nickase mutant
    Available since
    March 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62987-plasmid-map-sequence-id-435258

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  16. pCMV-Mm.cargo(Cas9)

    Plasmid
    #174865
    Purpose
    Expresses mouse Peg10 cargoRNA encoding SpCas9
    Depositor
    Feng Zhang
    Publication
    Segel et al Science. 2021 Aug 20;373(6557):882-889.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 flanked by MmPeg10 UTRs
    Tags
    HA-SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    174865-plasmid-map-sequence-id-342429

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  19. pSpCas9n(BB)-2A-Puro (PX462)

    Plasmid
    #48141
    Purpose
    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987
    Depositor
    Feng Zhang
    Publication
    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9n-2A-Puro
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Mutation
    Cas9 D10A nickase mutant
    Promoter
    Cbh
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  20. SP-dCas9-VPR

    Plasmid
    #63798
    Purpose
    SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector
    Depositor
    George Church
    Publication
    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SP-dCas9-VPR
    Use
    CRISPR and Synthetic Biology
    Tags
    VPR (VP64-p65-Rta)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    63798-plasmid-map-sequence-id-258220

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Showing: 201 - 220 of 14223 results