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pCMV-Neo-Bam APC

Plasmid

#16507

Depositor

Bert Vogelstein

Publication

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

APC (APC Human)

Expression

Mammalian

Promoter

CMV

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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FokI-dCas9

Plasmid

#52970

Purpose

Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

Depositor

David Liu

Publication

Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

Fok1 fused to dCas9

Use

CRISPR

Tags

Fok1 nuclease (homodimeric wildtype) and NLS

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

CMV

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pET-NLS-Cas9-6xHis

Plasmid

#62934

Purpose

Expression of NLS-Cas9-6xHis in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

NLS-Cas9-6xHis

Tags

6xHis tag and NLS

Expression

Bacterial

Promoter

T7

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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pBI-MCS-EGFP

Plasmid

#16542

Depositor

Bert Vogelstein

Publication

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Type

Empty backbone

Expression

Mammalian

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pBad-EBFP2

Plasmid

#14891

Purpose

Bacterial expression of EBFP2 blue fluorescent protein

Depositor

Robert Campbell

Publication

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Has service

Cloning Grade DNA

Insert

EBFP2

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCMV-GCaMP5G

Plasmid

#31788

Purpose

a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

Depositor

Douglas Kim, Loren Looger, GENIE Project

Publication

Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

Insert

GCaMP5G

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

45 Articles

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pRK5-HA-Ubiquitin-K33

Plasmid

#17607

Purpose

Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

Depositor

Ted Dawson

Publication

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K33 only, other lysines mutated to arginines; P19…

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pMXs-Nanog

Plasmid

#13354

Purpose

Retroviral expression of mouse Nanog

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

Nanog homeobox (Nanog Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Inducible Caspex expression

Plasmid

#97421

Purpose

For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics

Depositor

Steven Carr, Samuel Myers

Publication

Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4

Inserts

Caspex
T2A-Gfp

Use

CRISPR

Tags

3xFLAG and V5

Expression

Mammalian

Mutation

dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …

Promoter

TRE3G

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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Lentiviral-FOP-dGFP-reporter

Plasmid

#14885

Depositor

Tannishtha Reya

Publication

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

mutant LEF-1/TCF-responsive promoter (Ctnnb1 )

Use

Lentiviral

Tags

d2 eGFP

Expression

Mammalian

Mutation

mutant TCF/LEF binding sites

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGL3 BRE Luciferase

Plasmid

#45126

Depositor

Martine Roussel, Peter ten Dijke

Publication

Korchynskyi et al J Biol Chem. 2002 Feb 15;277(7):4883-91.

Insert

Id1-BRE

Use

Luciferase; Bmp/smad transcriptional reporter

Expression

Mammalian

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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pcDNA3 Connexin43-GFP-APEX2

Plasmid

#49385

Purpose

Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells

Depositor

Alice Ting

Publication

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

Connexin43 - EGFP - APEX2

Expression

Mammalian

Mutation

K14D, W41F, E112K, A134P on soybean APX; improved…

Promoter

CMV

Available since

Nov. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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CMV-GEM-GECO1

Plasmid

#32442

Purpose

Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Publication

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

GEM-GECO1

Expression

Mammalian

Mutation

GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDsRed-attP

Plasmid

#51019

Purpose

Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Publication

Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

Inserts

attP
LoxP-3xP3-DsRed-LoxP

Use

High copy, amp resistance

Promoter

3xP3 and None

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

88 Articles

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pEBFP2-Nuc

Plasmid

#14893

Purpose

Mammalian expression of EBFP2 blue fluorescent protein with NLS

Depositor

Robert Campbell

Publication

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Insert

pEBFP2-Nuc

Tags

NLS

Expression

Mammalian

Mutation

Replace EYFP of the vector pEYFP-Nuc from Clontec…

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pcDNA3/HA-SUMO3 (Sentrin 2)

Plasmid

#17361

Purpose

Mammalian expression of human SUMO3 with HA tag

Depositor

Edward Yeh

Publication

Kamitani et al J Biol Chem. 1998 Feb 6. 273(6):3117-20.

Insert

SUMO3 (SUMO3 Human)

Tags

HA

Expression

Mammalian

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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AAV-GFP/Cre

Plasmid

#49056

Purpose

Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.

Depositor

Fred Gage

Publication

Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

Insert

Cre

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

EGFP, Myc, and NLS

Expression

Mammalian

Promoter

CMV

Available since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pRK1043

Plasmid

#8835

Depositor

David Waugh

Publication

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

tobacco etch virus protease, catalytic domain

Tags

MBP and polyarginine

Expression

Bacterial

Mutation

Autolysis-resistant S219V mutant

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Laconic/pcDNA3.1(-)

Plasmid

#44238

Purpose

a genetically-encoded sensor for lactate concentration, transport and metabolic flux

Depositor

Luis Felipe Barros

Publication

San Martin et al PLoS One. 2013;8(2):e57712. doi: 10.1371

Insert

Laconic (LACtate Optical Nano Indicator from CECs)

Use

Lactate biosensor

Expression

Mammalian

Promoter

CMV

Available since

May 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

41 Articles

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pCAG-Cre-IRES2-GFP

Plasmid

#26646

Purpose

Mammalian coexpression of nls-Cre and EGFP from the CAG promoter

Depositor

Anjen Chenn

Publication

Woodhead et al J Neurosci. 2006 Nov 29;26(48):12620-30.

Insert

Cre

Use

Cre/Lox

Tags

GFP and IRES2

Expression

Mammalian

Promoter

CAG

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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