We narrowed to 15,270 results for: grn
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Plasmid#135958PurposeS. pyogenes sgRNA for +41 nick in RNF2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRNF2_+41 nicking sgRNA
ExpressionMammalianMutationSee manuscriptPromoterU6Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-U6-dgRNA-CAG-MPH
Plasmid#106259PurposeExpresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backboneDepositorAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-sgRosa26-1_CBh-Cas9-T2A-BFP
Plasmid#64216PurposeExpression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptideDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9
sgRNA targeting ROSA26-1
UseCRISPRTags3xFLAG, NLS, and T2A-BFPExpressionMammalianPromoterCBh and U6Available sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LentiGuide-Hygro
Plasmid#139462PurposeLentiviral vector with gRNA scaffold and hygromycin selectable markerDepositorInsertno sgRNA inserted; resistance gene: hygroR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMKO.1 puro p53 shRNA 1
Plasmid#10671DepositorPublicationAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA-eGFP-Reporter
Plasmid#60719PurposeExpresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA (5'-AAAGGTCGAGAAACTGCAAA-3')
UseCRISPRExpressionMammalianPromoterU6 and mouse U6Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGTR
Plasmid#63143PurposePCR template to construct polycistronic tRNA-gRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA-tRNA fusion
UseCRISPR and Synthetic BiologyAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAS9-mCherry-Frame +1
Plasmid#66940PurposeCRISPaint frame selector +1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA frame +1
Available sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Rictor_2 shRNA
Plasmid#1854DepositorAvailable sinceApril 7, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EGFP gRNA (BRDN0000563266)
Plasmid#80036Purpose3rd generation lentiviral gRNA plasmid targeting EGFPDepositorInsertEGFP
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAS9-mCherry-Frame +0
Plasmid#66939PurposeCRISPaint frame selector +0DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA frame +0
Available sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3-Basic-8x-gRNA-eGFP
Plasmid#60718PurposeeGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
Available sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330 p53
Plasmid#59910PurposepX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA targeting p53 (Trp53 Mouse)
UseCRISPRExpressionMammalianPromoterU6Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-UniSAM
Plasmid#99866PurposeEncodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUniSAM-mCherry + U6-gRNA2.0
UseCRISPR and Synthetic Biology; Dcas9-sam activationExpressionMammalianMutationBbsI sites in CDS were ablated by consensus mutat…PromoterEF1aAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD2-dU6:2gRNA
Plasmid#49409Purposeexpression of gRNA under control of the Drosophila U6:2 promoterDepositorInsertdU6-2:gRNA
UseCRISPRExpressionInsectPromoterdU6-2Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-(BbsI)_CBh-Cas9-T2A-BFP
Plasmid#64323PurposeExpression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptideDepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-EBFP2ExpressionMammalianPromoterCBh and U6Available sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W
Plasmid#67986PurposeCas9 activity reporter with mCherry and BFPDepositorInsertsU6gRNA cassette, PGKmCherryABFP cassette, WPRE
Guide RNA targeting modified BFP
UseCRISPR and LentiviralMutationDeleted BbsI site within WPRE, modified BFPAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA CAG
Plasmid#50927PurposeU6 driven sgRNA negative controlDepositorInsertnegative control sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAS9-mCherry-Frame +2
Plasmid#66941PurposeCRISPaint frame selector +2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA frame +2
Available sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO-Tet-On-shRNA-Control
Plasmid#98398PurposeLentivirus for expression of non-targeting control shRNA (Dox-inducible)DepositorInsertnon-targeting randomized sequence
UseLentiviralAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by