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Plasmid#188770PurposedCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)DepositorInsertdCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1)ExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCru5-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49218PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro human-GFP-N581K RLIM-IRES-mCherry
Plasmid#232244PurposeMammalian expression of N581K RLIM-GFP-IRES-mCherryDepositorInsertN581K RLIM-GFP-IRES-mCherry (RLIM Human)
UseTagsAcGFP1ExpressionMammalianMutationN581KPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro -GFP-I77P I78P REX1-IRES-mCherry
Plasmid#232245PurposeMammalian expression of I77P I78P REX1-GFP-IRES-mCherryDepositorInsertI77P I78P REX1-GFP-IRES-mCherry (Zfp42 Mouse)
UseTagsAcGFP1ExpressionMammalianMutationI77P I78PPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Myc(Δ1-143; ΔDeg1ΔDeg2)-GFP-IRES-mCherry (ΔTADΔDeg1ΔDeg2)
Plasmid#231234PurposeMYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and point mutations corresponding to the deletion of degron 1 and 2 for transient expression in mammalian cellsDepositorInsertMYC (MYC Human)
UseTagsExpressionMammalianMutationTAD deleted (Δ1-143), deletion of degron 1 & …PromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry
Plasmid#231007PurposeProtein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.DepositorInsertE4F1 aa220-242 (E4F1 Human)
UseTagsAcGFP1ExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ GFP-CDK5R1 degron (283-307)-IRES-mCherry
Plasmid#231008PurposeProtein stability reporter construct for CDK5R1 consisting of aa 283-307 for transient overexpression in mammalian cells.DepositorInsertCDK5R1 aa283-307 (CDK5R1 Human)
UseTagsAcGFP1ExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR-GAL4-UAS—sTNFaR-IRES-mCherry-PGK-BFP
Plasmid#223214PurposeLentiviral vector - mCherry reporter and sTNFaR for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorInsertSoluble TNFαR (TNFRSF1A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWPT-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49227PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag
Plasmid#216696PurposeSynchronize trafficking of TNFalpha from the ER (RUCH system)DepositorInsertsFIREmate-KDEL
Tumor Necrosis Factor alpha (TNFa)
UseTagsFIREtag, Streptavidin Binding Protein (SBP), and …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 K122 -ires-GFP-Osteocalcin-promoter-H2B mCherry reporter
Plasmid#68714DepositorInsertH2B mCherry and GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-mCherry
Plasmid#122959PurposeLentiviral expression of NOTCH2NL delta C terminus under CAG promoter with mCherry expressionDepositorInsertNOTCH2NL-delta C terminus (NOTCH2NLB Human)
UseLentiviralTags6xmyc tagExpressionMutationdeletion of C terminus from full length NOTCH2NLBPromoterCAG promoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-nls-mCherry-stop-IRES-myr-EGFP (JDW 1326)
Plasmid#229812PurposeA CAGGS driven expression vector containing an nls-mCherry followed by an IRES and then a myristoylated EGFP for labeling the cell membrane.DepositorInsertnls-mCherry
UseTagsSV40 NLSExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MYC(Δ1-143; Δ340-439)-GFP-IRES-mCherry (ΔTADΔDBD)
Plasmid#231235PurposeMYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and DNA-binding-domain (residues 340-439) for transient expression in mammalian cellsDepositorInsertMYC (MYC Human)
UseTagsExpressionMammalianMutationTAD deleted (delta1-143), DBD deleted (delta340-4…PromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-491 GFP[1] L3 Stuffer/Intein3 L4 GFP[2] IRES mCherry GFP(1-9)
Plasmid#130174PurposePPI (BiFC/TriFC) assay vectorDepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-490 GFP[1] L3 Stuffer/Intein2 L4 GFP[2] IRES mCherry GFP(1-9)
Plasmid#130173PurposePPI (BiFC/TriFC) assay vectorDepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-489 GFP[1] L3 Stuffer/Intein1 L4 GFP[2] IRES mCherry GFP(1-9)
Plasmid#130172PurposePPI (BiFC/TriFC) assay vectorDepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-488 GFP[1] L6 Stuffer/Intein4 L7 GFP[2] IRES mCherry GFP(1-9)
Plasmid#130171PurposePPI (BiFC/TriFC) assay vectorDepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-487 GFP[1] L6 Stuffer/Intein3 L7 GFP[2] IRES mCherry GFP(1-9)
Plasmid#130170PurposePPI (BiFC/TriFC) assay vectorDepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-486 GFP[1] L6 Stuffer/Intein2 L7 GFP[2] IRES mCherry GFP(1-9)
Plasmid#130169PurposePPI (BiFC/TriFC) assay vectorDepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2020AvailabilityAcademic Institutions and Nonprofits only