We narrowed to 7,572 results for: Lif

1. pCA528 SPASTIN , residues 112-196

   Plasmid

   #180627

   Purpose

   Bacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 112-196

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDM1383

   Plasmid

   #207423

   Purpose

   Extrachromosomal expression HSPC300-GFP and mCherry-Hs Raf1 (1–134 RBD)

   Depositor

   Rob Kay

   Article

   Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

   Inserts

   HSPC300
   Raf1 (RAF1 Human)
   

   Use

   Unspecified

   Tags

   GFP and mCherry

   Expression

   Mutation

   aa 1–134 (RBD) only

   Promoter

   Available since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-ROR1-V5/HIS

   Plasmid

   #205607

   Purpose

   Expression of human ROR1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   ROR1 receptor tyrosine kinase (ROR1 Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXPR_BRD003 sgKEAP1-2

   Plasmid

   #74986

   Purpose

   sgKEAP1-2, puromycin selection

   Depositor

   William Hahn

   Article

   Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

   Insert

   KEAP1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. plKO.1-DNAJC5-ShRNA

   Plasmid

   #205729

   Purpose

   Knockdown of DNAJC5

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   shRNA targeting DNAJC5 (DNAJC5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sema6d.1-AP-His

   Plasmid

   #72042

   Purpose

   Expresses the extracellular region of the Sema6D, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema6d.1 (Sema6d Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV mouse Vav1 IRES GFP

   Plasmid

   #107091

   Purpose

   Mammalian expression retroviral vector

   Depositor

   Martine Roussel, Steven Clifford

   Article

   Lindsey et al Oncogene. 2015 Sep 3;34(36):4746-57. doi

   Insert

   Vav1 (Vav1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTol1-UAS:Chronos-tdTomato

   Plasmid

   #124232

   Purpose

   Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin Chronos fused to the red fluorescent protein tdTomato

   Depositor

   Isaac Bianco

   Article

   Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

   Insert

   Chronos-tdTomato
   

   Use

   Zebrafish expression

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   14xUAS-E1b

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tom70NTD-ABHD16Acyto-mNG

   Plasmid

   #195159

   Purpose

   mammalian expression of outer mitochondrial membrane localized ABHD16A tagged with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

   Insert

   Tom70NTD (TOMM70 Human)
   

   Use

   Tags

   ABHD16A cytoplasmic domain and mNeonGreen

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5

    Plasmid

    #125236

    Purpose

    Expresses modified SPARK β2AR-NanoLuc transmembrane component in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5
    

    Use

    AAV

    Tags

    Flag, V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Sema6d.1-Fc-His

    Plasmid

    #72168

    Purpose

    Expresses the extracellular region of the Sema6D, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6d.1 (Sema6d Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. ANKRD24-TwinStrepII

    Plasmid

    #83190

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Article

    Morgan et al Elife. 2016 Aug 15;5:e18312. doi: 10.755

    Insert

    ANKRD24 (Ankrd24 Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-DNAJC5 (WT)-HaloTag

    Plasmid

    #205710

    Purpose

    Mammalian expression of DNAJC5 (WT)-HaloTag

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-HA-Nrxn1α-LNS2

    Plasmid

    #128106

    Purpose

    Express HA tagged Nrxn1α-LNS2

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS2 (Nrxn1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-GST-hFIP200(1363-1370Mut)-MBP

    Plasmid

    #190038

    Purpose

    Expression of FIP200 mutant

    Depositor

    James Hurley

    Article

    Shi et al Elife. 2020 Aug 10;9. pii: 59099. doi: 1

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Tags

    GST and MBP

    Expression

    Mammalian

    Mutation

    1363 RDKDLIES 1370 to GSSGGSSG

    Promoter

    CMV

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Sema3f(L,+)-AP-His

    Plasmid

    #72021

    Purpose

    Expresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains no deletion in exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3f (Sema3f Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. Arabidopsis thaliana Atm3 d80 pET21a+

    Plasmid

    #173045

    Purpose

    Expresses AtAtm3 in E.coli

    Depositor

    Douglas Rees

    Article

    Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

    Insert

    Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    with N-terminal 80 amino acids deletion

    Promoter

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-RNF110

    Plasmid

    #126547

    Purpose

    Expresses GFP-tagged RNF110 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    RNF110 (PCGF2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-HA-Nrxn1α-LNS4

    Plasmid

    #128108

    Purpose

    Express HA tagged Nrxn1α-LNS4

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS4 (Nrxn1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-HA-Nrxn1α-LNS5

    Plasmid

    #128109

    Purpose

    Express HA tagged Nrxn1α-LNS5

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS5 (Nrxn1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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