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Showing: 2201 - 2220 of 2453 results

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  1. pET-dHeFSpCas9-VP64-6xHis

    Plasmid
    #92119
    Purpose
    Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells
    Depositor
    Insert
    dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, NLS, and VP64
    Expression
    Bacterial
    Mutation
    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…
    Promoter
    T7
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. pDONR P4-P1R-V5-6xHis

    Plasmid
    #48346
    Purpose
    Contributes the coding sequence of a V5-6xHis epitope tags cassette as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Insert
    V5 and 6xHis epitope tags cassette
    Use
    Gateway entry clone
    Mutation
    Contains a Kozak sequence and initiation codon up…
    Promoter
    none
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
  3. pVE2_3_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_11xQUAS

    Plasmid
    #154223
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 11xQUAS+CP_pcbC
    Depositor
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available Since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pLM2_42_pgndA_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS

    Plasmid
    #154238
    Purpose
    Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_pcbC
    Depositor
    Insert
    pgndA:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    pGndA (An11g02040 A. niger)
    Available Since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. SBC015869

    Plasmid
    #226281
    Purpose
    Expresses MsCAD from trc promoter; expresses BsSfp and SrCAR from a separate trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.
    Depositor
    Inserts
    4'-phosphopantetheinyl transferase Sfp
    Cinnamyl alcohol dehydrogenase
    Carboxylic acid reductase
    Expression
    Bacterial
    Available Since
    Nov. 25, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. pDA2ScIfm

    Plasmid
    #193786
    Purpose
    Expresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selection
    Depositor
    Insert
    Frame-shifted CI
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PLTATAA synthetic promoter carrying binding sites…
    Available Since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pLA2ScIfmTcIfm

    Plasmid
    #193792
    Purpose
    Cassette 1: Expresses frame-shifted CI under PLLacO-1 promoter, Cassette 2: Expresses frame-shifted CI under PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selection
    Depositor
    Insert
    Two copies of frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PLLacO-1 and PLTetO-1
    Available Since
    April 13, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 2201 - 2220 of 2453 results