We narrowed to 14,605 results for: Ung;

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pCS6-SMASh-YFP

Plasmid

#68852

Purpose

Expresses SMASh tag fused to YFP at N terminus in mammalian cells

Depositor

Michael Lin

Article

Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

Insert

SMASh-YFP

Tags

SMASh tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCIP-AMPKa1_WT

Plasmid

#79010

Purpose

For mammalian expression of WT AMPK alpha1.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

AMPK alpha 1 (PRKAA1 Human)

Expression

Mammalian

Promoter

pCAGGs

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-M13-TEV-C-P2A-TdTomato

Plasmid

#92391

Purpose

produces AAV expressing Cal-light M13-TevC component and TdTomato

Depositor

Hyungbae Kwon

Article

Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

M13-TEV-C-P2A-TdTomato

Use

AAV and Synthetic Biology

Tags

TdTomato

Expression

Mammalian

Promoter

human synapsin

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCIP-AMPKa1_KD

Plasmid

#79011

Purpose

For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

AMPK alpha 1 (PRKAA1 Human)

Expression

Mammalian

Mutation

K47R

Promoter

pCAGGs

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET26_MraYaa

Plasmid

#100166

Purpose

recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Chung et al Science. 2013 Aug 30;341(6149):1012-1016

Insert

mraY (phospho-MurNAc-pentapeptide translocase)

Tags

MBP-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-4T MDM2 WT

Plasmid

#16237

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Tags

GST

Expression

Bacterial

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pSBbi-TIR1-tTA/Pur

Plasmid

#171683

Purpose

constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Inserts

tetracycline-controlled transactivator
Puromycin
EF-1a promoter

Expression

Mammalian

Promoter

EF-1a promoter and RPBSA promoter

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAW62.YY1.FKBP.knock-in.mCherry

Plasmid

#104370

Purpose

For knocking in FKBP F36V P2A mCherry onto YY1

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

FKBP12(F36V) -P2A-mCherry

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAW63.YY1.FKBP.knock-in.BFP

Plasmid

#104371

Purpose

For knocking in FKBP F36V P2A BFP

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

FKBP12(F36V) -P2A-BFP

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCH67 (crRNA expression vector)

Plasmid

#217337

Purpose

CROP-seq crRNA expression vector with GFP and puro marker

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

AsDR-filler-AsDR8, GFP-puro

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-DFCP1

Plasmid

#86746

Purpose

N-terminal mCherry-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Insert

human DFCP1 (ZFYVE1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRS41K-mCherry-sfGFP (pJJF001)

Plasmid

#173457

Purpose

C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-sfGFP

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-hAgo2

Plasmid

#21981

Depositor

Phil Sharp

Article

Leung et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

Insert

Argonaute 2 (AGO2 Human)

Expression

Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pLX304-Flag-APEX2-NES

Plasmid

#92158

Purpose

Cytosolic APEX2 construct

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

Flag-APEX2-NES

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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BPK2660

Plasmid

#70709

Purpose

Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 236HSC WT (BRCA2)

Plasmid

#16246

Depositor

Mien-Chie Hung

Article

Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

Insert

BRCA2 (BRCA2 Human)

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pCEP4-SERT-myc

Plasmid

#107456

Purpose

Mammalian expression plasmid for codon optimized hSERT with internal MYC tag inserted in ECL2

Depositor

Erik Procko

Article

Young et al bioRxiv 2021.04.19.440442

Insert

Serotonin Transporter (SERT) (SLC6A4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSQT1601

Plasmid

#53369

Purpose

human codon optimized Csy4 and FokI-dCas9 expression plasmid

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

hCsy4-T2A-NLS-hFokI-dCas9-NLS

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

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pCS6-YFP-SMASh

Plasmid

#68853

Purpose

Expresses SMASh tag fused to YFP at C terminus in mammalian cells

Depositor

Michael Lin

Article

Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

Insert

YFP-SMASh

Tags

SMASh tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAG001 His6-TwinStrep-SUMO-AapCas12b

Plasmid

#153162

Purpose

Bacterial expression plasmid for AapCas12b

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Joung et al medRxiv. 2020 May 8. doi: 10.1101/2020.0

Insert

AapCas12b

Expression

Bacterial

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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