We narrowed to 5,311 results for: cycs

1. pcDNA3.1-2xFLAG-2xSTREP (empty vector)

   Plasmid

   #172604

   Purpose

   Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Type

   Empty backbone

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPC-LaminA

   Plasmid

   #69059

   Purpose

   encodes the lamin A protein

   Depositor

   Gerardo Ferbeyre

   Article

   Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

   Insert

   LaminA (LMNA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

3. FSW-ALDH2-T2A-EGFP

   Plasmid

   #197036

   Purpose

   Express ALDH2 from hSyn1 promoter, co-expressed with EGFP

   Depositor

   Ronald Hart

   Article

   Sun et al Aging Cell. 2023 Feb;22(2):e13772. doi:

   Insert

   ALDH2 (ALDH2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV HA hRB delta CDK + T373

   Plasmid

   #58912

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with T373 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pFRT_TO_eGFP_CELF1

   Plasmid

   #106100

   Purpose

   Inducible gene expression of EGFP - CELF1

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

   Insert

   CELF1 (CELF1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV HA hRB delta CDK + S612

   Plasmid

   #58914

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S612 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with S612 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pCMV HA hRB delta CDK + S608

   Plasmid

   #58913

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S608 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with S608 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCAG-p21_WT

   Plasmid

   #40202

   Depositor

   Philipp Mergenthaler

   Article

   Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

   Insert

   p21 (CDKN1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CAG

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBItetDT480GFP

   Plasmid

   #96905

   Purpose

   tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 480 interrupted CTG repeats in exon 15

   Depositor

   Thomas Cooper

   Article

   Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   EGFP and human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
   

   Use

   Tetracycline responsive and bidirectional

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetracycline responsive and bidirectional

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Myc-Rab35 WT

    Plasmid

    #47433

    Depositor

    Peter McPherson

    Article

    Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

    Insert

    Rab35 (RAB35 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBItetDMPKSGFP

    Plasmid

    #96903

    Purpose

    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 0 CTG repeats in exon 15 located

    Depositor

    Thomas Cooper

    Article

    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    EGFP and human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
    

    Use

    Tetracycline responsive and bidirectional

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetracycline responsive and bidirectional

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-Rab7a-7

    Plasmid

    #56443

    Purpose

    Localization: Ras GTPase, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

    Insert

    Rab7a (RAB7A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N117S in NM_004637.5

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. px458_2A_GFP_sgRNA_ELAVL1

    Plasmid

    #106106

    Purpose

    CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    gRNA targeting ELAVL1 (ELAVL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUPI hT5-S322-Cyp

    Plasmid

    #80282

    Purpose

    Expresses human TRIM5 CypA fusion

    Depositor

    Jeremy Luban

    Article

    Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

    Insert

    TRIM5 CypA fusion (TRIM5, PPIA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICE-HA-CtIP-siR-WT

    Plasmid

    #82030

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    CtIP (RBBP8 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Mutations making the cDNA resistant to a siRNA

    Promoter

    CMV-tet

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHS1801

    Plasmid

    #205966

    Purpose

    JAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promoters

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH proteins and CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac (proteins), pJ23119 (crRNA)

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHS1232

    Plasmid

    #205969

    Purpose

    MWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promoters

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas5-HNH proteins and CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac (proteins), pJ23119 (crRNA)

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. tetO-MESP1

    Plasmid

    #170694

    Purpose

    doxycycline-inducible overexpression of MESP1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    MESP1 (MESP1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. 1037 pGL3 FasL promoter

    Plasmid

    #9028

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    Fas ligand promoter (FASLG Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pX330-NQL005-SOX2-sgRNA

    Plasmid

    #175553

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse SOX2 locus.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    spCas9-nuclease and sgRNA against mouse SOX2 STOP Codon
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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