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Plasmid#145649PurposeBacterial Expression plasmid for SARS-CoV-2 nsp9DepositorInsertSARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRS41K-mScarlet-I-mNeonGreen (pKBJ010)
Plasmid#173461PurposeC-SWAT donor for Seamless tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
DGK epsilon
Plasmid#35407DepositorInsertDGK epsilon (DGKE Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-H6-FGB
Plasmid#49203PurposeFluorescent reporter for cGMPDepositorInsertH6-FGB
UseTagsHis tag + Other tagsExpressionBacterial, Insect, and Mamm…MutationNonePromoterCMVAvailable sinceDec. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMB1-spect-PthrC-eGFP
Plasmid#107408PurposeThis plasmid contain promoter PthrC, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterPthrCAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NHERF1-PDZ1iP
Plasmid#28295DepositorInsertNHERF1 (NHERF1 Human)
UseTagsFLAGExpressionMammalianMutationContains aa 1-148 (deletion of PDZ2 and EB domain…PromoterAvailable sinceSept. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pZyxin-miRFP703
Plasmid#79999PurposeZyxin labeling with near-infrared fluorescent protein miRFP703DepositorInsertZyxin (ZYX Human)
UseTagsmiRFP703ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXGFPC-5 Pxyl-dcas9 (Spa)
Plasmid#133318Purposeexpresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus pasteurianus)
UseUnspecifiedTagsExpressionMutationPromoterxyloseAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
EFS eGFP hPGK BSD
Plasmid#188520PurposeBackbone for CRISPR interference (CRISPRi) isogenic PAM testingDepositorTypeEmpty backboneUseLentiviralTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pActinin-miRFP670nano
Plasmid#127432PurposeAlpha actinin labeling with near-infrared fluorescent proteinDepositorInsertAlpha-actinin-miRFP670nano
UseTagsmiRFP670nanoExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
P303-FBP_2
Plasmid#139693PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPHDepositorInsertp35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA-GFP-R5
Plasmid#106256PurposeConstitutively expresses GFP fused with silaffin-R5 silica affinity peptideDepositorInsertGreen fluorescent protein
UseTagsAdded silaffin R5 peptide to C terminus of GFP af…ExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
CeNL(Ca2+)_1.2μ-pcDNA3
Plasmid#111926PurposeCyan color bright luminescent calcium indicator with intermediate affinityDepositorInsertCeNL(Ca2+)_1.2μ
UseTagsExpressionMammalianMutationE67D, E104D, D133E at CaMPromoterCMVAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22-HT/prsA
Plasmid#73624PurposeProduces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tagDepositorInsertribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(Ca2+)_300/pcDNA3
Plasmid#51983Purposemammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nMDepositorInsertNano-lantern_CaM_E104Q-3GS
UseTagsExpressionMammalianMutationS257G in Rluc8PromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRnc-gGFP161
Plasmid#189536PurposeExpresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFPDepositorInsertsRNase III
gUTR-161-GFP
UseTagsExpressionBacterialMutationN/APromoterJ23119 and pBADAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPaxillin-Electra1-N1
Plasmid#179484PurposeExpresses paxillin fused to blue fluorescent protein Electra1DepositorInsertpaxillin-Electra1
UseTagsElectra1ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
OeNL(Ca2+)_18μ-pcDNA3
Plasmid#111928PurposeOrange color bright luminescent calcium indicator with intermediate affinityDepositorInsertOeNL(Ca2+)_18μ
UseTagsExpressionMammalianMutationE67D, E104D, D133E at CaMPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJL017
Plasmid#53532PurposeThis plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA geneDepositorInsertetpBA
UseTags6His and mycExpressionBacterialMutationPromoterpBADAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cyto-RA
Plasmid#209860PurposeDimerization dependent fluorescent protein RA fused to NESDepositorInsertddRFP A
UseTagsNES sequence NLVDLQKKLEELELDEQQExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only