We narrowed to 22,370 results for: ESC;

1. LbdCas12a KRAB

   Plasmid

   #188503

   Purpose

   Expresses FLAG tagged LbdCas12a KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   EF1a

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTubulin-miRFP670nano

   Plasmid

   #127429

   Purpose

   Human alpha-tubulin labeling with near-infrared fluorescent protein miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   miRFP670nano-human alpha-tubulin
   

   Tags

   miRFP670nano

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLifeAct-miRFP713

   Plasmid

   #136562

   Purpose

   F-actin labeling with a near-infrared fluorescent protein miRFP713

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   LifeAct-miRFP713
   

   Tags

   miRFP713

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. P305-FBP_4

   Plasmid

   #139695

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:tAtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   various

   Available Since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTJV1Sc3-rpoB

   Plasmid

   #166983

   Purpose

   Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

   Depositor

   Christopher Reisch

   Article

   Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

   Insert

   RecT (recT E. coli)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   pVvanCC

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKeratin-Electra1

   Plasmid

   #179480

   Purpose

   Keratin labeling with blue fluorescent protein Electra1

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   Keratin-Electra1
   

   Tags

   Electra1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKB817 (pBra-Hfq)

   Plasmid

   #174661

   Purpose

   Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.

   Depositor

   Ann Hochschild, Katherine Berry

   Article

   Berry et al Nucleic Acids Res. 2018 Jan 25;46(2):e12

   Insert

   Hfq
   

   Tags

   alpha NTD

   Expression

   Bacterial

   Promoter

   lpp/lacUV5 (tandem promoter)

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMAL-EFSep

   Plasmid

   #34627

   Depositor

   Jesse Rinehart, Dieter Söll

   Article

   Park et al Science. 2011 Aug 26;333(6046):1151-4.

   Insert

   EF-Sep (tufB E. coli)
   

   Tags

   6xHIS and MBP

   Expression

   Bacterial

   Mutation

   His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

   Available Since

   March 1, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pPaxillin-Electra2

   Plasmid

   #184926

   Purpose

   Expresses paxillin fused to blue fluorescent protein Electra2

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   paxillin-Electra2
   

   Tags

   Electra2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBW-m4046979

    Plasmid

    #145611

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. LAMP-CeNL_pcDNA3

    Plasmid

    #89514

    Purpose

    Cyan color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    CeNL
    

    Tags

    LAMP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBW-m4046920

    Plasmid

    #145717

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pRSETA-qmTQ2-citrate

    Plasmid

    #225251

    Purpose

    Bacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrate

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-citrate
    

    Tags

    6XHis, T7 tag, XpressTM tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXGFPC-5 Pxyl-dcas9 (Sth3)

    Plasmid

    #133317

    Purpose

    expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    dcas9 (Streptococcus thermophilus #3)
    

    Use

    Unspecified

    Promoter

    xylose

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMB-TMEr

    Plasmid

    #61208

    Purpose

    Fluorescent reporter for plasma membrane and peri-arbuscular membrane MtBCPsp-mCherry-BCP expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtBCPsp-mCherry-BCP
    

    Tags

    mCherry

    Expression

    Plant

    Promoter

    MtBCP1

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-NHERF1-PDZ1-2

    Plasmid

    #28294

    Depositor

    Maria-Magdalena Georgescu

    Article

    Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

    Insert

    NHERF1 (NHERF1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains aa 1-298 (deletion of the EB domain aa 2…

    Available Since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

17. LAMP-ReNL_pcDNA3

    Plasmid

    #89532

    Purpose

    Red color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    ReNL
    

    Tags

    LAMP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. BRAC/pcDNA3

    Plasmid

    #51967

    Purpose

    mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al PLoS One. 2010 Apr 1;5(4):e9935. doi: 10

    Insert

    BRAC
    

    Expression

    Mammalian

    Available Since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

19. DGK zeta

    Plasmid

    #35410

    Depositor

    Robert Lefkowitz, Stephen Prescott

    Article

    Nelson et al Science. 2007 Feb 2;315(5812):663-6.

    Insert

    DGK zeta (DGKZ Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

20. Mito-LSSmGFP

    Plasmid

    #186536

    Purpose

    Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to mitochondria

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP
    

    Tags

    Human COX8a first 29 amino acids

    Expression

    Mammalian

    Mutation

    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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