19,824 results
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Plasmid#119554PurposeCRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide)DepositorInsertNOS_NLS_GB1_noNLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV puro Tet VSVG
Plasmid#82724PurposeLentiviral plasmid that expresses VSVG protein under a doxycycline-inducible promoterDepositorInsertVSVG
UseLentiviralExpressionMammalianAvailable SinceOct. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-AT1R-APEX
Plasmid#96847PurposeFor expression of AT1R-APEX in mammalian cellsDepositorInsertAT1R-APEX (AGTR1 Human)
ExpressionMammalianAvailable SinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHen2 Avi-tag-linker-p3
Plasmid#119819PurposeFor cloning and subsequent expression of peptide fragments fused with Avi-tag (for biotinylation) and with pIII protein of M13K07 bacteriophageDepositorInsertlinker with Avi-tag and p3
TagsAvi-tagExpressionBacterialAvailable SinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7B2Jnk1a1
Plasmid#19726DepositorAvailable SinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
LV-Sox10MCS5-GFP-EF1a-mCherry
Plasmid#115782PurposeSox10-MCS5-GFP and a constitutive EF1α-mCherry reporter plasmid. Construct generates constitutive red fluorescence and basal promoter cfos conjugated SOXMCS5 enhancer driven GFP expression in a cell.DepositorInsertsmouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
EF1alpha promoter driven constitutive mCherry
UseLentiviralTagsEF1α promoter fused to mCherry and cfos promoter …PromoterSOX10 Multiple Species Conserved enhancer element…Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-flag-myr-Akt1-IRES-GFP
Plasmid#65063Purposemammalian retroviral expression plasmidDepositorAvailable SinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
CRY2(L348F)-CreN
Plasmid#75368PurposeExpresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinaseDepositorInsertCRY2 (CRY2 Mustard Weed)
TagsCre recombinase, residues 19-104ExpressionMammalianMutationL348FPromoterCMVAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas9(D10A)
Plasmid#74495PurposeNicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10ADepositorInsertCas9 D10A
UseSynthetic BiologyExpressionBacterialMutationD10APromoterpTetoAvailable SinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
3544 pMIG Bcl-2
Plasmid#8793DepositorAvailable SinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only