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8,734 results

Showing: 2261 - 2280 of 8734 results

  2. EF1a_mCherry_P2A_Hygro

    Plasmid
    #135003
    Purpose
    Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    135003-plasmid-map-sequence-id-272070

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  3. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    137729-plasmid-map-sequence-id-272646

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  6. Lamp1-mNeonGreen

    Plasmid
    #98882
    Purpose
    In vivo visualization of lysosomes (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    Lamp1
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    98882-plasmid-map-sequence-id-191165

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  11. lentiCRISPR - EGFP sgRNA 1

    Plasmid
    #51760
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51760-plasmid-map-sequence-id-392672

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  13. pOpen_taq

    Plasmid
    #153315
    Purpose
    Bacterial expression of Taq polymerase
    Depositor
    Drew Endy
    Insert
    Taq polymerase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tac
    Available since
    June 2, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153315-plasmid-map-sequence-id-298774

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  15. pRubiG-T2A-Cas9

    Plasmid
    #75348
    Purpose
    Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Bryan Luikart
    Article
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR and Retroviral
    Tags
    GFP (via t2a)
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    75348-plasmid-map-sequence-id-142640

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Showing: 2261 - 2280 of 8734 results