8,812 results

1. pDEST17-Cdc34a

   Plasmid

   #18674

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Cdc34A (CDC34 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

2. Cbh_v5 AAV-ABE N-terminal

   Plasmid

   #137177

   Purpose

   AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-ABE N-terminal
   

   Use

   AAV

   Mutation

   Cas9 D10A

   Promoter

   Cbh

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDA_550

   Plasmid

   #203398

   Purpose

   All-in-one lentiviral expression of gRNA and EnAsCas12a

   Depositor

   John Doench

   Article

   Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

   Insert

   Enhanced Acidaminococcus Cas12a
   

   Use

   CRISPR and Lentiviral

   Promoter

   EF1a

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHIV-EGFP

   Plasmid

   #21373

   Purpose

   self-inactivating lentiviral plasmid for co-expression of your gene of interest and EGFP

   Depositor

   Bryan Welm, Zena Werb

   Article

   Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 19, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-Myc-IRES-RFP

   Plasmid

   #35395

   Depositor

   Martine Roussel

   Article

   Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

   Inserts

   Myc (Myc Mouse)
   RFP
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 5, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pSBbi 41BBL-CD86 mIL-15

   Plasmid

   #183245

   Purpose

   Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

   Depositor

   Alexander McLellan

   Article

   Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

   Insert

   41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)
   

   Expression

   Mammalian

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGFP-Rab8A

   Plasmid

   #24898

   Depositor

   Maxence Nachury

   Article

   Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

   Insert

   hRab8A
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPSU250

   Plasmid

   #173837

   Purpose

   Expresses 250 kD Penn State ladder protein

   Depositor

   Song Tan

   Article

   Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

   Insert

   STRHSTPABPACIL1bQRSSTRHSTPABMBPpepN
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

   Plasmid

   #103871

   Purpose

   dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Inserts

   PspCas13b
   huADAR2DD (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   GS and HIV NES

   Expression

   Mammalian

   Mutation

   Changed histidne 133 to alanine and histidine 105…

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-CMV-3xFLAG-gateway-EGFP

    Plasmid

    #122845

    Purpose

    N-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. MBP-FUS_FL_WT

    Plasmid

    #98651

    Purpose

    Expresses MBP-tagged full length FUS

    Depositor

    Nicolas Fawzi

    Article

    Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

    Insert

    FUS (FUS Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Available Since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDIRECT_22C

    Plasmid

    #91135

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5 beta4

    Plasmid

    #16037

    Depositor

    Filippo Giancotti

    Article

    Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

    Insert

    beta4 (ITGB4 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVDL9.3

    Plasmid

    #168299

    Purpose

    Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

    Depositor

    Víctor de Lorenzo

    Article

    Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

    Insert

    Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
    

    Expression

    Bacterial

    Promoter

    LacI-Plac

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-All-in-one-hCascade

    Plasmid

    #134919

    Purpose

    Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSOUP

    Plasmid

    #165419

    Purpose

    The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

    Article

    Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

    Insert

    Rep A
    

    Use

    Helper plasmid required for replication for all p…

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-GFP-NMHC II-A

    Plasmid

    #11347

    Depositor

    Robert Adelstein

    Article

    Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

    Insert

    NMHC II-A (MYH9 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAV1-mCherry

    Plasmid

    #27705

    Depositor

    Ari Helenius

    Article

    Hayer et al Traffic. 2010 Mar . 11(3):361-82.

    Insert

    caveolin-1 (CAV1 Canis lupus familiaris)
    

    Tags

    monomeric Cherry

    Expression

    Mammalian

    Available Since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. CD44S pBabe-puro

    Plasmid

    #19127

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    CD44 standard (CD44 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti PGK Hygro DEST (w530-1)

    Plasmid

    #19066

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Hygro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge