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Showing: 2261 - 2280 of 7988 results

  1. ATP1A1_G8_Dual_sgRNA

    Plasmid
    #178104
    Purpose
    Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G8 sgRNA + user-specified sgRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178104-plasmid-map-sequence-id-350193

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  5. pLJM60 FLAG-RAB35 wt

    Plasmid
    #107104
    Purpose
    Lentiviral vector that codes for FLAG-RAB35 wildtype (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.
    Depositor
    Charles Sawyers
    Article
    Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi
    Insert
    RAB35 (RAB35 Human)
    Use
    Lentiviral
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    wildtype
    Promoter
    CMV
    Available Since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    107104-plasmid-map-sequence-id-205812

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  20. H52-pUCM-hNGN2-inv

    Plasmid
    #110492
    Purpose
    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm
    Depositor
    Michael Ward
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    mcherry
    rtTA3G
    Use
    CRISPR, Cre/Lox, and TALEN
    Expression
    Mammalian
    Promoter
    CAG, EF-1 alpha, and TRE3G
    Available Since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    110492-plasmid-map-sequence-id-207872

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Showing: 2261 - 2280 of 7988 results