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pCIS GEM-CEPIA1er

Plasmid

#58217

Purpose

Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

GEM-CEPIA1er

Tags

myc

Expression

Mammalian

Mutation

GEM-GECO1 E298D F359W D400E E407D

Promoter

CAG

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-RLuc8.6-535

Plasmid

#87125

Purpose

Mammalian expression vector expressing red shifted variant of the reporter gene-Renilla luciferase (Rluc8.6)

Depositor

Sanjiv Sam Gambhir

Article

Loening et al Nat Methods. 2007 Aug;4(8):641-3. Epub 2

Insert

Renilla Luciferase

Expression

Mammalian

Mutation

RLuc8/ A123S/D154M/E155G/D162E/I163L/V185L.

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLIB

Plasmid

#80610

Purpose

pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Promoter

polyhedrin

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCI-EGFP-NR2b wt

Plasmid

#45447

Depositor

Andres Barria, Robert Malinow

Article

Barria et al Neuron. 2002 Jul 18;35(2):345-53.

Insert

NR2B (Grin2b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

EGFP inserted after the predicted signal peptide …

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCI-EGFP-NR2a wt

Plasmid

#45445

Depositor

Andres Barria, Robert Malinow

Article

Barria et al Neuron. 2002 Jul 18;35(2):345-53.

Insert

NR2A (Grin2a Rat)

Tags

EGFP

Expression

Mammalian

Mutation

EGFP inserted after the predicted signal peptide …

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCm C-term MBP NikJC199U

Plasmid

#174365

Purpose

Expression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

Depositor

Brandon Greene

Article

Caceres et al Methods Enzymol. 2022;662:119-141. doi:

Insert

nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]

Tags

8xHis, MBP, and TEV

Expression

Bacterial

Mutation

C199U

Promoter

T7

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pQE30-roGFP1-iR

Plasmid

#83314

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

Insert

roGFP1-iR

Tags

6xHIS

Expression

Bacterial

Mutation

ro1(C48S/S147C/Q204C) plus X insertion, iR betwe…

Promoter

T5

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pJYS1Peftu

Plasmid

#85546

Purpose

Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

Depositor

Sheng Yang

Article

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Inserts

Cpf1
recT

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Expression

Bacterial

Promoter

etfu and unknown

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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MAC-tag-N

Plasmid

#108078

Purpose

Gateway compatible destination vectors containing StrepIII/HA/BioID

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

CCDB (ccdB(letD) )

Tags

StrepIII-HA-BirA*

Expression

Bacterial and Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3_cAMPFIRE-M

Plasmid

#182280

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-M (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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eMagB-iRFP-Mito

Plasmid

#162250

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagB-iRFP-OMP25

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-tag

Plasmid

#55180

Purpose

FLAG-tag

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

FLAG

Use

Synthetic Biology; Bacillus biobrick box

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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RelA cFlag pcDNA3

Plasmid

#20012

Depositor

Stephen Smale

Article

Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

Insert

RelA (Rela Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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Gg 3kb Green opsin GFP

Plasmid

#72919

Purpose

green opsin promoter from chicken (Gallus gallus) gDNA chr26:4,504,913–4,501,931 in galGal4 driving GFP

Depositor

Joseph Corbo

Article

Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

Insert

chicken green opsin promoter

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL2-FLAG

Plasmid

#15819

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin2 (CUL2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N427

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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ER-mCherry-eMagA

Plasmid

#162248

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

CytP450 ER targeting sequencel-mCherry-eMagA

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pmScarlet3-H_I3

Plasmid

#239343

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3-H in mammalian cells. Assembles into nanocages tagged with 60 FPs.

Depositor

Torsten Wittmann

Article

Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

Insert

I3-01

Tags

mScarlet3-H (a.k.a. mYongHong)

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pICH-chl-roGFP2-synORP1

Plasmid

#191701

Purpose

Ti plasmid pICH86988 harbouring chl-roGFP2-synORP1 for H2O2 detection in the plastids of plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

chl-roGFP2-synORP1

Use

Binary agrobacterium ti plasmid

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-C1-CAMKIIalpha

Plasmid

#21226

Depositor

Tobias Meyer

Article

Shen et al Neuron. 1998 Sep . 21(3):593-606.

Insert

CAMKIIalpha (Camk2a Rat)

Tags

GFP

Expression

Mammalian

Mutation

Lab plasmid KS 0001.

Available Since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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pCI-EGFP-NR1 wt

Plasmid

#45446

Depositor

Andres Barria, Robert Malinow

Article

Barria et al Neuron. 2002 Jul 18;35(2):345-53.

Insert

NR1 (Grin1 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

EGFP inserted after the predicted signal peptide …

Promoter

CMV

Available Since

June 11, 2013

Availability

Academic Institutions and Nonprofits only

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