We narrowed to 67,243 results for: nin

1. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

   Plasmid

   #202571

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Use

   Tags

   GFP-AI retrotransposition reporter and HaloTag7 o…

   Expression

   Mammalian

   Mutation

   Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 Flag TSC2 (T1462A)

   Plasmid

   #14130

   Depositor

   Brendan Manning

   Article

   Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

   Insert

   TSC2 T1462A (TSC2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T1462A

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pATLIC

   Plasmid

   #110287

   Purpose

   A backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)

   Depositor

   In-Geol Choi

   Article

   Ko et al Appl Environ Microbiol. 2012 May;78(9):3

   Insert

   yfaL
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD promoter

   Available since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG_EGFP-RandE3

   Plasmid

   #79870

   Purpose

   Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-RandE3
   

   Use

   Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Beta-actin

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. JDW 1050 (pME_V5_GATA1_P2A_H2A_mCherry)

   Plasmid

   #232127

   Purpose

   A middle entry gateway clone containing V5 tagged human GATA1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   GATA1 (GATA1 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Dlx-FLEX-hM4Di-mCherry

   Plasmid

   #209701

   Purpose

   Expresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent manner

   Depositor

   Peer Wulff

   Article

   Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

   Insert

   hM4D(Gi)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJFRC-20XUAS-IVS-PhiC31

   Plasmid

   #133565

   Purpose

   Replace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   PhiC31 Recombinase (int )
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   20X UAS

   Available since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-EF1a-EGFP-RAMP4-IRES-Puromycin

   Plasmid

   #134863

   Purpose

   Expresses EGFP-tagged endoplasmic reticulum marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   RAMP4 (SERP1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPBT-peRNA_GG-Puro

   Plasmid

   #173220

   Purpose

   piggyBac transposon containing hU6 promotor and pegRNA cloning site

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

   Inserts

   pegRNA cloning cassette
   PuroR
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a and hU6

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pL1-pKPI-MtLAP1-Adh

    Plasmid

    #193520

    Purpose

    Anthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula roots

    Depositor

    Jeremy Murray

    Article

    Kumar et al New Phytol. 2022 Sep 20. doi: 10.1111/np

    Insert

    MtLAP1
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Medtr8g059790(KPI)

    Available since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCB6-Cor1-HA

    Plasmid

    #102815

    Purpose

    Expression of Coronin1 in eukaryotic cells

    Depositor

    Jean Pieters

    Article

    Gatfield et al Mol Biol Cell. 2005 Jun;16(6):2786-98. E

    Insert

    TACO/Coronin 1 (Coro1a Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMALp2x-CARP-1

    Plasmid

    #41727

    Depositor

    Koji Okamoto

    Article

    Nakano et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

    Insert

    clam ADP-ribosylating protein-1
    

    Use

    Bacterial subcloning

    Tags

    Expression

    Mutation

    deleted 5'-UTR nucleotides 1-64

    Promoter

    pMB1

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)

    Plasmid

    #47869

    Purpose

    All-in-one plasmid targeting tdTomato

    Depositor

    Erik Sontheimer, James Thomson

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Inserts

    NmCas9
    U6pr-tracrRNA
    tdTomato crRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. actc1b-mKate2-FAPP1

    Plasmid

    #109503

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3). Binds PtdIns4P

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-mKate2-FAPP1
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJG85

    Plasmid

    #89281

    Purpose

    Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

    Depositor

    Daniel Voytas

    Article

    Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. AYBEv0.1

    Plasmid

    #193966

    Purpose

    A wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    ecTadA8e-nSpCas9(D10A)-MPG(wt)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, EF1a, CBh

    Available since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-Myo9b R1695M

    Plasmid

    #134911

    Purpose

    Expression of rat Myo 9b (Myr 5) in mammalian cells.GAP minus mutant.

    Depositor

    Martin Bähler

    Article

    Muller et al Mol Biol Cell. 1997 Oct;8(10):2039-53. d

    Insert

    Myo 9b (rat) with 3' UTR. GAP minus mutant R1695M (Myo9b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    changed arginine 1695 to methionine

    Promoter

    CMV IE

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHS1528

    Plasmid

    #205982

    Purpose

    U6-MWEE01000013 Cas5-HNH DR-BpiI-DR

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas5-HNH DR-BpiI-DR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS433-pCSDest2-AcrIIC1Nme

    Plasmid

    #85679

    Purpose

    Mammalian expression of Type II-C anti-CRISPR protein AcrIIC1Nme from Neisseria meningitidis

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    Type II-C anti-CRISPR AcrIIC1Nme
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMuLE ENTR MCS L3-L2

    Plasmid

    #62088

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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