We narrowed to 4,395 results for: TIL

1. pTS2311-Tier1-spacer-PPGK-NLuc_TB

   Plasmid

   #169552

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-NanoLuc Luciferase - mTagBFP fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5 FRT/TO Plk4 ∆24-mCherry

   Plasmid

   #80271

   Purpose

   mammalian expression plasmid for Plk4 with multi-phospho degron deleted

   Depositor

   Andrew Holland

   Article

   Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

   Insert

   Plk4 (PLK4 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   deletion of multi-phospho degron (amino acids 282…

   Promoter

   CMV

   Available since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-GFP(S65T)-His3MX6

   Plasmid

   #41598

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   His3
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   May 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. NAX_CAGGS_CAS9_m4_KRAB_mcherry

   Plasmid

   #167878

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

5. CVS-N2c-mTurquoise

   Plasmid

   #172376

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   mTurquoise
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-mouse vinculin full length (889)

   Plasmid

   #67935

   Purpose

   Mammalian expression of mouse vinculin with GFP fusion

   Depositor

   Alpha Yap

   Article

   Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

   Insert

   Vinculin (Vcl Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   L159P in vinculin

   Promoter

   CMV

   Available since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-IRES-Gαi*-BERKY3

   Plasmid

   #158424

   Purpose

   BRET biosensor for detection of Gαi-GTP in lentiviral vector pLVX-IRES-Hyg

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
   

   Use

   Lentiviral

   Tags

   myc

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. mApple-MyosinIIA-C-18

   Plasmid

   #54929

   Purpose

   Localization: Cytoskeleton, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Article

   Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

   Insert

   MyosinIIA
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

9. iTol2Amp-γ-crystallin:RFP

   Plasmid

   #108455

   Purpose

   This plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.

   Depositor

   Nadia Mercader Huber

   Article

   Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

   Insert

   Tol2-Amp-y-cryst-mCherry
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT2Apuro-Stargazin-mEGFP-iLID

    Plasmid

    #178529

    Purpose

    Expresses Stargazin-mEGFP-iLID in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Stargazin-mEGFP-iLID
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. mpx:UtrCH-GFP

    Plasmid

    #45247

    Depositor

    Anna Huttenlocher

    Article

    Yoo et al Dev Cell. 2010 Feb 16. 18(2):226-36.

    Insert

    utrophin (UTRN Human)
    

    Use

    Zebrafish expression

    Tags

    EGFP

    Expression

    Mutation

    contains amino acids 1-261 of NP_009055.2; Q236R

    Promoter

    mpx

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21-pelB-LaG-16

    Plasmid

    #172746

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRFP-NLS

    Plasmid

    #105296

    Purpose

    To visualize nuclear dynamics in living cells

    Depositor

    Kenneth Yamada

    Article

    Petrie et al Science. 2014 Aug 29;345(6200):1062-5. d

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    aa encoding 172-192

    Promoter

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-BCR-ABL1-p190-IRES-EGFP

    Plasmid

    #219964

    Purpose

    Express human BCR-ABL1-P190 in mammalian cells

    Depositor

    Mohamed Fareh

    Insert

    BCR-ABL1-P190 (ABL1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. MMLV-CAG-TVA-IRES-Puro

    Plasmid

    #172366

    Purpose

    Generation of TVA-expressing stable cell lines, resistant to the antibiotic Puromycin

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + Puro
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21-pelB-LaG-16-4GS-LaG-2

    Plasmid

    #172764

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16-4GS-LaG-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS-Stargazin-mEGFP-iLID

    Plasmid

    #178523

    Purpose

    Expresses Stargazin-mEGFP-iLID in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Stargazin-mEGFP-iLID
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWJB9

    Plasmid

    #126650

    Purpose

    Expresses AdrA from a modified pLAFR3

    Depositor

    Ute Romling

    Article

    Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

    Insert

    adrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30
    

    Use

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-EF1-IRES-NEO deleted ITR

    Plasmid

    #67906

    Purpose

    PiggyBac transposon encoding Neomycin resistance with deleted inverted terminal repeat

    Depositor

    Alex Kentsis

    Article

    Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

    Insert

    NeoR (neoR Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. LC201: pMVP/PB/Puro-DEST

    Plasmid

    #121874

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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