We narrowed to 4,857 results for: Mela

1. pCB6-HFE-EGFP

   Plasmid

   #12104

   Depositor

   Pamela Bjorkman

   Article

   Giannetti et al J Biol Chem. 2004 Jun 11. 279(24):25866-

   Insert

   HFE (HFE Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pEGFP-gfap(Intron1/5'/Exon1-zebrafish)

   Plasmid

   #39761

   Depositor

   Pamela Raymond

   Article

   Bernardos et al Gene Expr Patterns. 2006 Oct;6(8):1007-1

   Insert

   zebrafish gfap promoter- EGFP (gfap Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   gfap

   Available Since

   Aug. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV5-myc-Erk2

   Plasmid

   #39222

   Depositor

   Melanie Cobb

   Article

   Xu et al J Biol Chem. 2001 Jul 13;276(28):26509-1

   Insert

   Erk2 (Mapk1 Rat)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pJK506

   Plasmid

   #155382

   Purpose

   dCas12a (D917A) + PA4-mVenus. (removed crRNAs)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Promoter

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. NpT7-5-Erk2

   Plasmid

   #39230

   Depositor

   Melanie Cobb

   Article

   Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

   Insert

   Erk2 (Mapk1 Rat)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pCB6HindIII-rat FcRn

   Plasmid

   #121671

   Purpose

   Expresses rat neonatal Fc receptor in mammalian cells

   Depositor

   Pamela Bjorkman

   Article

   Tesar et al Traffic. 2006 Sep;7(9):1127-42.

   Insert

   rat neonatal Fc receptor (Fcgrt Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. NpT7-5-ERK1

   Plasmid

   #39229

   Depositor

   Melanie Cobb

   Article

   Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

   Insert

   ERK1 (MAPK3 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pLib6.6

   Plasmid

   #176652

   Purpose

   Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLib6.4B

   Plasmid

   #176665

   Purpose

   Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLib6.6B

    Plasmid

    #176666

    Purpose

    Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEM-WingGFP-tan

    Plasmid

    #83473

    Purpose

    EGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

    Depositor

    Patricia Wittkopp

    Article

    Lamb et al Fly (Austin). 2016 Aug 5:1-12.

    Insert

    WingGFP-tan
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Hsp70 and enhancer from D. melanogaster yellow ge…

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJK413

    Plasmid

    #155372

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPS833

    Plasmid

    #1358

    Depositor

    Pamela Silver

    Article

    Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

    Insert

    GSP1 (GSP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    G21V, stabilizes the GTP-bound form

    Promoter

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pJK429

    Plasmid

    #155376

    Purpose

    PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUASt-ssBiP-ESCargo*

    Plasmid

    #140165

    Purpose

    Expresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogaster

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    ssBip-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hs70

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPS835

    Plasmid

    #8933

    Depositor

    Pamela Silver

    Article

    Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

    Insert

    GSP1 (GSP1 Budding Yeast)
    

    Use

    Tags

    myc

    Expression

    Yeast

    Mutation

    G21V, stabilizes the GTP-bound form

    Promoter

    Available Since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pJK412

    Plasmid

    #155371

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK430

    Plasmid

    #155377

    Purpose

    PphlF_A2NT in dCRv1, 2x cascade + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A2NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCEP4-HA-MEKK1-1-1493

    Plasmid

    #21632

    Depositor

    Melanie Cobb

    Article

    Xu et al Proc Natl Acad Sci U S A. 1995 Jul 18. 9

    Insert

    mitogen-activated protein kinase kinase kinase 1 (Map3k1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pET32a-TRXtag

    Plasmid

    #11516

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    None - thioredoxin is vector encoded (trxA )
    

    Use

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    vector modified; removed N-terminal His tag by di…

    Promoter

    Available Since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

21. pB[U6-3 wex2 PUb-DsRed]

    Plasmid

    #169010

    Purpose

    For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    white ex2 sgRNA
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster U6:3

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p3xFlag-CMV7-Erk2

    Plasmid

    #39223

    Depositor

    Melanie Cobb

    Article

    Robinson et al J Biol Chem. 2002 Apr 26;277(17):14844-5

    Insert

    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p3xFlag-CMV7-Erk2_KR

    Plasmid

    #39224

    Depositor

    Melanie Cobb

    Article

    Robinson et al J Biol Chem. 2002 Apr 26;277(17):14844-5

    Insert

    Erk2(K52R) (Mapk1 Rat)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    K52R

    Promoter

    CMV

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

24. pJK511

    Plasmid

    #155384

    Purpose

    dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDH-EF1a-tetR-T2A-iRFP

    Plasmid

    #176028

    Purpose

    construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    tetR
    iRFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJK503

    Plasmid

    #155380

    Purpose

    dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available Since

    May 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCEP4L-Erk1

    Plasmid

    #39225

    Depositor

    Melanie Cobb

    Article

    Frost et al Proc Natl Acad Sci U S A. 1994 Apr 26;91

    Insert

    Erk1 (MAPK3 Human)
    

    Use

    Episomal

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pCDH-EF1a-tetR-T2A-tSapphire

    Plasmid

    #176312

    Purpose

    construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-Sapphire

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    tetR
    T-sapphire
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. RP78 pUAST Orco mRNA

    Plasmid

    #53431

    Purpose

    Expresses the full-length Drosophila melanogaster Orco cDNA under Gal4/UAS control

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    Orco (Orco )
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Aug. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pJK526

    Plasmid

    #155387

    Purpose

    PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA4 in dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJK528

    Plasmid

    #155388

    Purpose

    dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJK434

    Plasmid

    #155378

    Purpose

    PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJK422

    Plasmid

    #155373

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJK423

    Plasmid

    #155374

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAcUW51-CgE

    Plasmid

    #13762

    Depositor

    Pamela Bjorkman

    Article

    Sprague et al PLoS Biol. 2006 Jun . 4(6):e148.

    Insert

    HSV-1 glycoprotein E
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    C-terminal region of the HSV-1 gE (KOS strain ect…

    Promoter

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

36. pJK520

    Plasmid

    #155386

    Purpose

    PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA1 in dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. CAHS2 (pBS0249)

    Plasmid

    #185139

    Purpose

    For the mammalian expression of the tardigrade protein CAHS2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pB[vasa-Cas9 PUb-ZsGreen]

    Plasmid

    #169012

    Purpose

    For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    Cas9
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster vasa

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pB[nos-Cas9 PUb-ZsGreen]

    Plasmid

    #169011

    Purpose

    For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    Cas9
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster nanos

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MTNR1A-DuET

    Plasmid

    #213348

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MTNR1A (MTNR1A Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge