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Showing: 2281 - 2300 of 4162 results

  4. pDuRCC-N

    Plasmid
    #81194
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please view depositor comments below and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81194-plasmid-map-sequence-id-160426

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  8. Barcode backbone

    Plasmid
    #158665
    Purpose
    This plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combination
    Depositor
    Ravid Straussman
    Article
    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    hcRED
    Expression
    Mammalian
    Available Since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    158665-plasmid-map-sequence-id-309764

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  9. pHD157

    Plasmid
    #84033
    Purpose
    Vector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the fabp10 promoter and Venus under the crystalin promoter in zebrafish
    Depositor
    Daniel Hesselson, Didier Stainier
    Article
    Ni et al Proc Natl Acad Sci U S A. 2012 Sep 18;10
    Inserts
    Cre
    Venus
    Use
    Cre/Lox; Zebrafish transgenesis
    Tags
    mCherry
    Promoter
    Crystalin and fabp10
    Available Since
    Nov. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    84033-plasmid-map-sequence-id-166816

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  11. pCAG_EGFP-RandE3

    Plasmid
    #79870
    Purpose
    Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.
    Depositor
    Don Arnold
    Article
    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm
    Insert
    GFP-RandE3
    Use
    Synthetic Biology
    Tags
    GFP and HA
    Expression
    Mammalian
    Promoter
    Beta-actin
    Available Since
    Aug. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    79870-plasmid-map-sequence-id-149706

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  14. pEGFP-N1-mCherry-ApoB-TagBFP

    Plasmid
    #129383
    Purpose
    Plasmid bearing the editing target of APOBEC1 fused between the mCherry and the Tag-BFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of Tag-BFP
    Depositor
    Silvestro Conticello
    Article
    Chieca et al Methods Mol Biol. 2021;2181:69-81. doi:
    Insert
    mCherry-ApoB-Tag-BFP
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    129383-plasmid-map-sequence-id-254328

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Showing: 2281 - 2300 of 4162 results