4,133 results

1. pSLQ1651-sgTelomere(F+E)

   Plasmid

   #51024

   Purpose

   Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres

   Depositor

   Bo Huang, Stanley Qi

   Article

   Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

   Insert

   Optimized sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6 promoter

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 2C::tdTomato Reporter

   Plasmid

   #40281

   Depositor

   Samuel Pfaff

   Article

   Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

   Insert

   2C Promoter (2-cell stage promoter)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pVSV eGFP dG

   Plasmid

   #31842

   Depositor

   Connie Cepko

   Article

   Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   VSV genome
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pU6-Sp-pegRNA-HEK3_CTT_ins

   Plasmid

   #132778

   Purpose

   S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   HEK3_CTT_ins pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   U6

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBiFC-bFosVC155

   Plasmid

   #22013

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   bFosVC155
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Oct. 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET17_K560_GFP_His

   Plasmid

   #15219

   Depositor

   Ron Vale

   Article

   Woehlke et al Cell. 1997 Jul 25. 90(2):207-16.

   Insert

   Human neuronal kinesin heavy chain (KIF5B Human)
   

   Use

   Tags

   GFP and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BPK764

   Plasmid

   #65767

   Purpose

   Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Flag-HA-USP5

   Plasmid

   #22590

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP5 (USP5 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRK5-HA-Ubiquitin-K6

   Plasmid

   #22900

   Depositor

   Sandra Weller

   Article

   Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K6 only, all other lysines mutated to arginines

   Promoter

   Available since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDESTmycIGF2BP3

    Plasmid

    #19879

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mCherry-P4M-SidM

    Plasmid

    #51471

    Purpose

    Biosensor for PtdIns4P

    Depositor

    Tamas Balla

    Article

    Hammond et al J Cell Biol. 2014 Apr 7.

    Insert

    P4M-SidM(546-647)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. NFAT/AP-1 3x luciferase

    Plasmid

    #11783

    Depositor

    Anjana Rao

    Article

    Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

    Insert

    NFAT/AP-1 3x (Il2 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTE4398

    Plasmid

    #74042

    Purpose

    Expresses human codon-optimized LbCpf1 and Lb crRNA.

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    Lb crRNA
    LbCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mRFP-Rab7

    Plasmid

    #14436

    Purpose

    Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.

    Depositor

    Ari Helenius

    Article

    Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

    Insert

    Rab 7 (RAB7A Canis familialis)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    3 silent mutations near the C-terminus

    Promoter

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p2NIL

    Plasmid

    #20188

    Depositor

    Tanya Parish

    Article

    Parish et al Microbiology (Reading). 2000 Aug;146 ( P

    Type

    Empty backbone

    Use

    Suicide vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Flag-hPIASx beta

    Plasmid

    #15210

    Depositor

    Ke Shuai

    Article

    Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

    Insert

    PIASx beta (PIAS2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mCherry-Vimentin-N-18

    Plasmid

    #55158

    Purpose

    Localization: Intermediate Filaments, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. IKK-2 WT

    Plasmid

    #11103

    Depositor

    Anjana Rao

    Article

    Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

    Insert

    IKK-2 (IKBKB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET His6 GFP TEV LIC cloning vector (1GFP)

    Plasmid

    #29663

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    6xHis, GFP, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-TRIM28

    Plasmid

    #45568

    Depositor

    Fanxiu Zhu

    Article

    Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    G34S mutation in EGFP

    Promoter

    CMV

    Available since

    May 29, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge