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pLJM1-FLAG-metap2

Plasmid

#100510

Purpose

Expresses FLAG tagged metap2 in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

METAP2 (METAP2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-basP:fLuc-TK:rLuc

Plasmid

#138369

Purpose

Lentiviral plasmid contains basal promoter driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

sequence multi cloning sites, minP driving firefly luciferase and TK promoter driving renilla luciferase

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ9714: pHR-PGK-SV40_NLS-dCasMINI-V4-NZF-c-Myc_NLS-mCherry-WPRE

Plasmid

#203220

Purpose

Expression of dCasMINI-V4-NZF-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCasMINI-V4-NZF-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSecTag-ATX

Plasmid

#17839

Depositor

Steven Rosen

Article

Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

Insert

Autotaxin (ENPP2 Human)

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
mTagGFP2-Rab5a-7

Plasmid

#56417

Purpose

Localization: Ras GTPase, Excitation: 483, Emission: 506

Depositor

Michael Davidson

Insert

Rab5a

Tags

mTagGFP2

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mTLR2

Plasmid

#13083

Depositor

Ruslan Medzhitov

Insert

TLR2 (Tlr2 Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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LgBiT-hACE2

Plasmid

#173431

Purpose

protein expression plasmid of LgBiT-hACE2-IgG1 Fc

Depositor

David Nemazee

Article

Huang et al J Immunol. 2021 Jul 1;207(1):344-351. do

Insert

LgBiT-hACE2-IgG1 Fc (ACE2 Human)

Use

Luciferase

Tags

IgG1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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WDR5-HaloTag

Plasmid

#200881

Purpose

Mammalian expression of human WDR5 with C-terminal HaloTag fusion

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

WDR5-HaloTag (WDR5 Human)

Expression

Mammalian

Mutation

WT

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pPT24-mtnluc

Plasmid

#184339

Purpose

Transformation of mitochondrial DNA in yeast

Depositor

Martin Ott

Article

Rzepka et al STAR Protoc. 2022 May 25;3(2):101359. do

Insert

nanoluciferase recoded for mitochondrial expression flanked by COX2 UTRs

Expression

Yeast

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_7C11-1 heavy chain

Plasmid

#198251

Purpose

recombinant expression of heavy chain of monoclonal anti-AMP antibody 7C11-1

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

heavy chain of monoclonal anti-AMP antibody 7C11-1 (mouse IgG2a)

Tags

IL10 signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-Nanobody

Plasmid

#70696

Purpose

Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

mCherry-Nanobody

Tags

GST

Expression

Bacterial

Available Since

March 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-3xFLAG-TERT

Plasmid

#51631

Purpose

Mammalian expression of 3xFLAG tagged TERT, N-terminal.

Depositor

Steven Artandi

Article

Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

Insert

TERT (TERT Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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NmCas9_sgRNA_expression_in_pBluescript

Plasmid

#122091

Purpose

U6 driven NmCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

NmCas9 tracrRNA (NEWENTRY S. pyogenes, Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mTurquoise2

Plasmid

#99113

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mTurquoise2

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pGP-pcDNA3.1 Puro-CAG-Voltron2

Plasmid

#172909

Purpose

Mammalian expression of voltage sensor

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Insert

Voltron2

Expression

Mammalian

Mutation

A122D

Promoter

CAG

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pWalium20-10XUAS-3XFLAG-dCas9-VPR

Plasmid

#78897

Purpose

Expresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for in vivo CRISPRa

Depositor

Norbert Perrimon

Article

Lin et al Genetics. 2015 Oct;201(2):433-42. doi: 1

Insert

dCas9-VPR

Tags

3xFLAG

Expression

Insect

Promoter

10XUAS

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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mTagGFP2-Clathrin-15

Plasmid

#56413

Purpose

Localization: Clathrin Vesicles, Excitation: 483, Emission: 506

Depositor

Michael Davidson

Insert

Clathrin (CLTB Human)

Tags

mTagGFP2

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pF420

Plasmid

#123659

Purpose

Produces cofactor F420 in E. coli

Depositor

Colin Jackson

Article

Bashiri et al Nat Commun. 2019 Apr 5;10(1):1558. doi:

Inserts

CofD
FbiB
FbiD
FbiC

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_R0040

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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CROP-seq-mTurquoise2-v2-no-scaffold

Plasmid

#228522

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using mTurquoise2 as selection marker

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Insert

mTurquoise2

Use

Lentiviral

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJK1

Plasmid

#111722

Purpose

to express human BLM protein in yeast cells

Depositor

Ian Hickson

Article

Karow et al J Biol Chem. 1997 Dec 5;272(49):30611-4.

Insert

BLM (BLM Human)

Tags

hexahistidine

Expression

Yeast

Mutation

a C-terminal hexahistidine tag was added, a yeast…

Promoter

GAL1

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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