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We narrowed to 7,692 results for: Lif;

Showing: 5751 - 5800 of 7692 results
  1. PMXS-GOT1

    Plasmid
    #72872
    Purpose
    The retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.
    Depositor
    Insert
    GOT1 (GOT1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    sgRNA resistant human GOT1, 7 silent mutations c1…
    Available Since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. pLenti PGK mKate2-OGT D554N (Puro)

    Plasmid
    #154292
    Purpose
    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, D554N variant, no Ser/Thr glycosylation)
    Depositor
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Lentiviral
    Tags
    2x FLAG and mKate2
    Expression
    Mammalian
    Mutation
    D554N mutation (Ser/Thr glycosylation-incompetent)
    Promoter
    PGK
    Available Since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pCW57.1 KBTBD4 R313PRR

    Plasmid
    #184626
    Purpose
    Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 R313PRR expression in mammalian cells
    Insert
    N-terminal Flag tagged KBTBD4 R313PRR (KBTBD4 Human)
    Use
    Lentiviral; Doxycycline inducible
    Expression
    Mammalian
    Mutation
    R313PRR
    Promoter
    TRE promoter, Tet ON
    Available Since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pLenti PGK mKate2-OGT 5N5A (Puro)

    Plasmid
    #154293
    Purpose
    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage)
    Depositor
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Lentiviral
    Tags
    2x FLAG and mKate2
    Expression
    Mammalian
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations; HCF-…
    Promoter
    PGK
    Available Since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. pCW57.1 KBTBD4 P311PP

    Plasmid
    #184625
    Purpose
    Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 P311PP expression in mammalian cells
    Insert
    N-terminal Flag tagged KBTBD4 P311PP (KBTBD4 Human)
    Use
    Lentiviral; Doxycycline inducible
    Expression
    Mammalian
    Mutation
    P311PP
    Promoter
    TRE promoter, Tet ON
    Available Since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pDONR P2R-P3-IRES_ECFP

    Plasmid
    #48354
    Purpose
    Contributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules.
    Insert
    IRES_ECFP
    Use
    Gateway entry vector
    Mutation
    ECFP translation is mediated by an IRES. Contains…
    Promoter
    none
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
  7. Desmoplakin II donor only control (F40-based no force control)

    Plasmid
    #118718
    Purpose
    The donor (YPet(short)) only control for the no force control of the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.
    Depositor
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)-F40-mCherry(Y72L)] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    truncation after aa1353; Y72L mutation in mCherry…
    Promoter
    CMV
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. Desmoplakin II donor only control (FL-based tension sensor)

    Plasmid
    #118723
    Purpose
    The donor only (YPet(short)) control for the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.
    Depositor
    Insert
    human Desmoplakin II-[YPet(short)-FL-mCherry(Y72L)] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-FL-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    inserted FL-based tension sensor module after aa1…
    Promoter
    CMV
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. MB 94C thsS(t3)R-Bxb1_P7-sfGFP_mCherry

    Plasmid
    #232471
    Purpose
    Optimized thiosulfate sensor with recombinase switch and fluorescent reporter (GFP), constitutive mCherry
    Depositor
    Inserts
    thsS(t3)
    thsR
    PphsA342
    sfGFP
    AxeTxe
    mCherry
    Bxb1 integrase
    Tags
    ssrA degradation tag
    Expression
    Bacterial
    Mutation
    Contains the following mutations K286Q, Q350H, I…
    Promoter
    J23100 (TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC), J23…
    Available Since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. Desmoplakin II donor only control (FL-based no force control)

    Plasmid
    #118722
    Purpose
    The donor (YPet(short)) only control for the no force control of the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.
    Depositor
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry(Y72L)] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-FL-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    truncation after aa1353; Y72L mutation in mCherry…
    Promoter
    CMV
    Available Since
    Dec. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. Desmoplakin II donor only control (F40-based tension sensor)

    Plasmid
    #118717
    Purpose
    The donor (YPet(short)) only control for the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.
    Depositor
    Insert
    human Desmoplakin II-[YPet(short)-F40-mCherry(Y72L)] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    CMV
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 5751 - 5800 of 7692 results