We narrowed to 21,628 results for: ESC

1. Nano-lantern-peroxisome/pcDNA3

   Plasmid

   #51974

   Purpose

   mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to peroxisomes

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Use

   Tags

   SKL

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

2. Nano-lantern(Ca2+)_300/pRSETB

   Plasmid

   #51977

   Purpose

   bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM_E104Q-3GS
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

3. CT-Nano-lantern(ATP1)/pRSETB

   Plasmid

   #51990

   Purpose

   bacterial expression of a Nano-lantern-based luminescent ATP indicator, targeted to chloroplasts

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_ATP1
   

   Use

   Tags

   6xHis and chloroplast transit peptid

   Expression

   Bacterial

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pACYC-Skp-hLYZ

   Plasmid

   #83924

   Purpose

   Co-expresses cytoplasmic copies of the Skp protein folding chaperone and wild type human lysozyme..

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Skp
   lysozyme (LYZ Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGBW-m4046876

   Plasmid

   #145669

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;Myc

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pGBW-m4046726

   Plasmid

   #145756

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 3C-like proteinase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pGBW-m4046945

   Plasmid

   #145668

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pGBW-m4046947

   Plasmid

   #145705

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pGBW-m4046804

   Plasmid

   #145595

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp8

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pGBW-m4046766

    Plasmid

    #145612

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pCMB-TMEg

    Plasmid

    #61209

    Purpose

    Fluorescent reporter for plasma membrane and trunk peri-arbuscular membrane MtBCPsp-GFP-BCP expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtBCPsp-GFP-BCP
    

    Use

    Tags

    EGFP

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. P315-FBP_10

    Plasmid

    #139701

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing CPH and Npga

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pUBQ1:NnH3H:tNos, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Nano-lantern(Ca2+)_11/pRSETB

    Plasmid

    #51981

    Purpose

    bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=11nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM-4GS
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pMB1-spect-PthrC3-eGFP

    Plasmid

    #107409

    Purpose

    This plasmid contain promoter PthrC3, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

    Depositor

    Kang Zhou

    Article

    Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PthrC3

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. CT-Nano-lantern(ATP1)/pENTR1A

    Plasmid

    #51991

    Purpose

    Gateway entry vector for Nano-lantern-based luminescent ATP indicator, targeted to chloroplasts

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_ATP1
    

    Use

    Entry vector

    Tags

    chloroplast transit peptid

    Expression

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pGBW-m4046926

    Plasmid

    #145663

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA

    Plasmid

    #133344

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_cpaA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    constitutive

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Nano-lantern(ATP1)/pRSETB

    Plasmid

    #51988

    Purpose

    bacterial expression of a Nano-lantern-based luminescent ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_ATP1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

19. Nano-lantern(Ca2+)_63/pcDNA3

    Plasmid

    #51986

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-4GS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pTEI128

    Plasmid

    #126209

    Purpose

    Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x3 and expression in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    ccdB Cassette (BsaI)
    GFP11x3 (5 aa linker)
    

    Use

    Binary, module 1

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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