We narrowed to 18,420 results for: REV

1. pCfB8153

   Plasmid

   #175204

   Purpose

   Xenopus oocyte expression vector containing YBR235W. Contains T7 RNA polymerase binding site, and adds polyA tail to YBR235W. Used for generation of mRNA, for injection into xenopus laevis oocytes.

   Depositor

   Irina Borodina

   Article

   Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

   Insert

   YBR235W
   

   Use

   Mrna expression, injection into xenopus oocytes

   Tags

   PolyA

   Promoter

   T7

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB8154

   Plasmid

   #175205

   Purpose

   Xenopus oocyte expression vector containing YBR287W. Contains T7 RNA polymerase binding site, and adds polyA tail to YBR287W. Used for generation of mRNA, for injection into xenopus laevis oocytes.

   Depositor

   Irina Borodina

   Article

   Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

   Insert

   YBR287W
   

   Use

   Mrna expression, injection into xenopus oocytes

   Tags

   PolyA

   Promoter

   T7

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB8155

   Plasmid

   #175206

   Purpose

   Xenopus oocyte expression vector containing YCL002C. Contains T7 RNA polymerase binding site, and adds polyA tail to YCL002C. Used for generation of mRNA, for injection into xenopus laevis oocytes.

   Depositor

   Irina Borodina

   Article

   Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

   Insert

   YCL002C
   

   Use

   Mrna expression, injection into xenopus oocytes

   Tags

   PolyA

   Promoter

   T7

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFB8455

   Plasmid

   #175207

   Purpose

   Xenopus oocyte expression vector containing YCR075C. Contains T7 RNA polymerase binding site, and adds polyA tail to YCR075C. Used for generation of mRNA, for injection into xenopus laevis oocytes.

   Depositor

   Irina Borodina

   Article

   Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

   Insert

   YCR075C
   

   Use

   Mrna expression, injection into xenopus oocytes

   Tags

   PolyA

   Promoter

   T7

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. 186_pETcon_SARS2_XBB1-5

   Plasmid

   #208086

   Purpose

   yeast surface display of the SARS-CoV-2 XBB.1.5 variant RBD

   Depositor

   Tyler Starr

   Article

   Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

   Insert

   SARS-CoV-2 XBB.1.5 RBD (S Budding Yeast, SARS-CoV-2 virus)
   

   Tags

   HA and c-Myc

   Expression

   Yeast

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPbuCas13b-gRNA-1

   Plasmid

   #184567

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKP105 (pRS416-YBR139Wp-YBR139W-PA-ADH1t)

   Plasmid

   #106461

   Purpose

   Expresses Atg42/Ybr139w with a C-terminal PA tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   YBR139W
   

   Tags

   PA

   Expression

   Bacterial and Yeast

   Promoter

   YBR139W

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKP135 (pRS406-YBR139Wp-YBR139W-PA-ADH1t)

   Plasmid

   #106470

   Purpose

   Expresses Atg42/Ybr139w with a C-terminal PA tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   YBR139W
   

   Tags

   PA

   Expression

   Bacterial and Yeast

   Promoter

   YBR139W

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNIA-CEN-FLAG-LINXa4-Bre1

   Plasmid

   #73617

   Purpose

   Control of the E3 ligase Bre1 with LINXa in yeast.

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

   Insert

   LINXa4-Bre1dNLS
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Bre1 point mutations K8A, K9A and K11A

   Promoter

   ADH1

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Gal4pBS134-Pleum

    Plasmid

    #60339

    Purpose

    Contains a promoter of three gal binding sites derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Expression

    Yeast

    Available Since

    Feb. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNA4-rB90

    Plasmid

    #18959

    Depositor

    Mariano Viapiano

    Article

    Viapiano et al J Neurooncol. 2008 Jul . 88(3):261-72.

    Insert

    Brevican C-terminus (Bcan Rat)
    

    Expression

    Mammalian

    Mutation

    Rat brevican C-terminus: Signal peptide aa 1-22 …

    Available Since

    Dec. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

12. T7opt in pCAGGS

    Plasmid

    #65974

    Purpose

    Human codon-optimized T7 polymerase

    Depositor

    Benhur Lee

    Article

    Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

    Insert

    T7-opt
    

    Expression

    Mammalian

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pML104

    Plasmid

    #67638

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    pSNR52 and pTDH3

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNESx2-EGFP-C1-PABDx2-Spo20

    Plasmid

    #220082

    Purpose

    PA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear export

    Depositor

    Gerry Hammond

    Article

    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

    Insert

    Spo20 (SPO20 Budding Yeast)
    

    Tags

    X. leavis map2k1.L(32-44)-GGSG-X. leavis map2k1.L…

    Expression

    Mammalian

    Mutation

    amino acids 51-91, tandem dimer

    Promoter

    CMV

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pML107

    Plasmid

    #67639

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    pSNR52 and pTDH3 (aka GAP promoter)

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Braf

    Plasmid

    #40775

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    Braf (BRAF Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Starting Met removed

    Promoter

    CMV

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEVA1

    Plasmid

    #58953

    Purpose

    Contains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.

    Depositor

    Thomas Fox, Thomas Mason

    Article

    Sanchirico et al EMBO J. 1998 Oct 1;17(19):5796-804.

    Insert

    VAR1u, VAR1 recoded for expression from the nucleus
    

    Use

    Yeast ecoli shuttle vector

    Tags

    preCox4 mitochondrial matrix targeting signal

    Expression

    Yeast

    Mutation

    Synthetic gene in standard genetic code

    Promoter

    ADH1

    Available Since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pPlacPbuCas13b-gRNA-T-deGFP

    Plasmid

    #184840

    Purpose

    Expression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    single-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119 and lac promoter

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPlacPbuCas13b-gRNA-NT

    Plasmid

    #184838

    Purpose

    Expression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Co-expression of single-spacer CRISPR array with a nontargeting spacer for PbuCas13b and PbuCas13b nuclease in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119 and lac promoter

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCSN068

    Plasmid

    #101749

    Purpose

    Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Inserts

    Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Promoter

    Heterologous TEF1 promoter from A. gossypii. and …

    Available Since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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