Addgene

We narrowed to 23,428 results for: Spr;

Showing: 2301 - 2320 of 23428 results

Results per page:

Sort results by:

p201B Cas9

Plasmid

#59177

Purpose

Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
BAR

Use

CRISPR

Tags

Expression

Mutation

Promoter

2x35S

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330GFP-hU6-gRNA-hSpCas9

Plasmid

#128385

Purpose

GFP positive gRNA/hSpCas9 construct

Depositor

Budd A. Tucker

Article

Giacalone et al Curr Protoc Stem Cell Biol. 2018 Feb 28;

Insert

GFP, gRNA and hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-TO-nmdCas9-3XGFP

Plasmid

#64109

Purpose

Expression of Nm dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
NM9-SpCas9-NLS3

Plasmid

#128177

Purpose

Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

SpCas9-NLS3

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

pPGK1

Available since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pChimera

Plasmid

#61476

Purpose

Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

U6-26:sgRNA

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

U6-26

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTarget12f1

Plasmid

#171183

Purpose

Constitutive expression of sgRNA without donor DNA

Depositor

Kohsuke Honda

Article

Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

Insert

sgRNA scaffold in the CRISPR/Cas12f1 system

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119(SpeI) promoter

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pKm-HABE

Plasmid

#170332

Purpose

a modularized single-plasmid adenosine base editing (A-to-G) tool for Sinorhizobium meliloti

Depositor

Deqiang Duanmu

Article

Wang et al Front Microbiol. 2021 Jun 18;12:686008.

Insert

Rhizobium codon-optimized ABEs

Use

CRISPR

Tags

Expression

Mutation

Cas9n-D10A

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBx-Spas-sgRNA-Gm

Plasmid

#105232

Purpose

Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosa

Depositor

Kristala Prather

Article

Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

Insert

S. pasteurianus sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCasTns(Ptr)-IS186

Plasmid

#175581

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(IS186)

Use

Tags

Expression

Bacterial

Mutation

NO

Promoter

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ-dpcoCas9-TV

Plasmid

#158409

Purpose

CRISPR-dpcoCas9 fused with TV activator for transcriptional activation

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dpcoCas9-TV(6xTAL-2xVP64)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS263

Plasmid

#122379

Purpose

CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon

Depositor

Rebecca Shapiro

Article

Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

Insert

HSP90

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTns(Ptr)

Plasmid

#175576

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTnsABC

Use

Tags

Expression

Bacterial

Mutation

NO

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pV1200

Plasmid

#111429

Purpose

CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)

Depositor

Gerald Fink

Article

Vyas et al Sci Adv. 2015;1(3):e1500248.

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGG>nls-hCas9-nls

Plasmid

#99138

Purpose

Expresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryos

Depositor

Marianne Bronner

Article

Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

Insert

humanized Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Chicken beta actin (CAG)

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS267

Plasmid

#122380

Purpose

CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon

Depositor

Rebecca Shapiro

Article

Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

Insert

HSP90

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK43 pHomL-TDH3p-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179027

Purpose

Parent vector for markerless yeast integration cassettes with untagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-TO-dCas9-GFP

Plasmid

#64106

Purpose

Expression of Sp dCas9-GFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRH2521

Plasmid

#84380

Purpose

Expression of sgRNA from a imyc promoter (Pimyc)

Depositor

Robert Husson

Article

Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

Insert

sgRNA cloning site

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBLO1811_Cas9_noNLS_human

Plasmid

#74490

Purpose

Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-sgRNA-Lib

Plasmid

#53121

Purpose

sgRNA expression construction in a 3rd generation lentiviral backbone

Depositor

Wensheng Wei

Article

Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge