We narrowed to 37,794 results for: KAN

1. pET-45b-HOXA13(+7A)-IDR-mCherry

   Plasmid

   #145285

   Purpose

   Bacterial Expression of 6xHis HOXA13(+7A)IDR mCherry Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXA13(+7A)-IDR (HOXA13 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A-repeat expanded

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-45b-HOXA13(WT)-IDR-mCherry

   Plasmid

   #145284

   Purpose

   Bacterial Expression of 6xHis HOXA13(WT) IDR mCherry Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXA13(WT)-IDR (HOXA13 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGAL4-DBD-HOXD11-Adel-IDR

   Plasmid

   #145241

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and HOXD11-Adel IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD11-Adel-IDR (HOXD11 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A-repeats deleted

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGAL4-DBD-HOXA13+7A-IDR

   Plasmid

   #145239

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and HOXA13+7A IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXA13+7A-IDR (HOXA13 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A-repeat expanded

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGAL4-DBD-HEY2wt-IDR

   Plasmid

   #145235

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and HEY2wt IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HEY2wt-IDR (HEY2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGAL4-DBD-GSX2wt-IDR

   Plasmid

   #145233

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and GSX2wt IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   GSX2wt-IDR (GSX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-HA-DEPTOR S286/287/291A

   Plasmid

   #159160

   Purpose

   Expresses DEPTOR S286/287/291A mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

   Insert

   DEPTOR (DEPTOR Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed serine 286,287 and 291 to alanine

   Promoter

   CMV

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-HA-DEPTOR S286/287/291D

   Plasmid

   #159161

   Purpose

   Expresses DEPTOR S286/287/291D mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

   Insert

   DEPTOR (DEPTOR Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed serine 286,287 and 291 to aspartic acid

   Promoter

   CMV

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGAL4-DBD-EOMES-Adel-IDR

   Plasmid

   #145230

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and EOMES-Adel IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   EOMES-Adel-IDR (EOMES Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A-repeats deleted

   Promoter

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGAL4-DBD-HOXD13wt-IDR

    Plasmid

    #145244

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXD13wt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13wt-IDR (HOXA13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGAL4-DBD-GSX2-Adel-IDR

    Plasmid

    #145232

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and GSX2-Adel IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    GSX2-Adel-IDR (GSX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKLV-gRNA-Bcan-Ntrk1_4

    Plasmid

    #136413

    Purpose

    Lentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.

    Depositor

    Massimo Squatrito

    Article

    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

    Insert

    U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKLV-gRNA-Myb-Qk_1

    Plasmid

    #136415

    Purpose

    Lentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP.

    Depositor

    Massimo Squatrito

    Article

    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

    Insert

    U6_sgRNA(Myb)_U6_sgRNA(Qk)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 289aa Met133, 138, 186Ileu ELL2

    Plasmid

    #127272

    Purpose

    Expresses 289aa human ELL2 with Met133, 138, 186Ileu

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 w Met to ILeus stopped at XbaI (ELL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Met133, 138, 186 Ileu, cut with XbaI blunt fillin

    Promoter

    pEF1a

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. frameshift ELL2

    Plasmid

    #127257

    Purpose

    For in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    XhoI site between the 1st &2nd Met was filled…

    Promoter

    T7

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 564aa ELL2 bluescript

    Plasmid

    #127267

    Purpose

    For in vitro translation of human ELL2 lacking COOH region

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Addgene Plasmid 127256--SphI site filled in, then…

    Promoter

    T7

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TRAV29

    Plasmid

    #123401

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRAV29 (TRAV29DV5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMIR-192-Reporter

    Plasmid

    #71872

    Purpose

    Mammalian expression vector for the analysis of miR-19α activity

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    Reverse complementary sequence of miR-192
    

    Use

    Tags

    firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pMIR-193a-5p-Reporter

    Plasmid

    #71873

    Purpose

    Mammalian expression vector for the analysis of miR-193a-5p activity

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    Reverse complementary sequence of miR-193a-5p
    

    Use

    Tags

    firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pMIR-31-192-193a-Reporter

    Plasmid

    #71874

    Purpose

    Mammalian expression vector for the analysis of miR-31-19α-193a activity

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    Binding sequence targeted by miR-31, miR192, and miR-193a-5p
    

    Use

    Tags

    firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pGL403

    Plasmid

    #119953

    Purpose

    Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

    Inserts

    gpps
    limS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminal truncation of 56 amino acid residues (…

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-hARΔ142–337

    Plasmid

    #89106

    Purpose

    Mammalian expression of human androgen receptor with deletion of amino acids 142-337 N-terminal transactivation function 1

    Depositor

    Elizabeth Wilson

    Article

    Titus et al PLoS One. 2012;7(1):e30192. doi: 10.1371

    Insert

    hAR delta 142-337 (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hAR delta 142-337

    Promoter

    CMV

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

23. CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro

    Plasmid

    #83306

    Purpose

    Lentivirus vector to express guideRNA and dCas9 with puro resistant gene

    Depositor

    Tohru Kimura

    Article

    Murakami et al Nat Immunol. 2021 Mar;22(3):301-311. doi

    Insert

    sgRNA and dCas9 from pX330
    

    Use

    Lentiviral

    Tags

    FLAG and PA tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for sgRNA and CBh for dCas9

    Available since

    Dec. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLKO-Tet-On-shRNA-Control

    Plasmid

    #98398

    Purpose

    Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    non-targeting randomized sequence
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

    Plasmid

    #107270

    Purpose

    Expresses Cas9 upon induction with doxicycline.

    Depositor

    Pablo Menéndez

    Article

    Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

    Inserts

    hSpCas9
    rtTA
    bGH polyA-SV40polyA
    

    Use

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and TRE

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216246

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgAAVS1.5
    

    Use

    CRISPR; Human safe harbor locus (a avs1) site-spe…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pX037

    Plasmid

    #167156

    Purpose

    MoClo-compatible Level2 vector for autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz and nnCPH. Does not contain NpgA.

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    caffeic acid cycle genes
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pNOS, p35s

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-Ef1a-DIO-Sun1GFP-WPRE-pA

    Plasmid

    #160141

    Purpose

    Cre- dependent expression of Sun1GFP for nuclei isolation

    Depositor

    Andreas Pfenning

    Article

    Lawler et al J Neurosci. 2020 Nov 10. pii: JNEUROSCI.

    Insert

    Sun1-GFP (Sun1 Mouse)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N-truncated: removed amino acids 1-207, retained …

    Promoter

    Ef1a

    Available since

    March 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-hGPR56-IRES-GFP

    Plasmid

    #52297

    Purpose

    Expresses human GPR56 and GFP in mammalian cells.

    Depositor

    Christopher A Walsh

    Article

    Bae et al Science. 2014 Feb 14;343(6172):764-8. do

    Insert

    Human GPR56 coding sequence (ADGRG1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3/hArf6(WT)-mCherry

    Plasmid

    #79422

    Purpose

    Expresses C-terminally mCherry-tagged Arf6(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLX302_FOXA1-V5

    Plasmid

    #70090

    Purpose

    Expression of human FOXA1 with C-terminal V5 tag, puromycin selection marker, 3rd generation lentiviral vector

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    FOXA1 (FOXA1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV-Myc-HNF4A

    Plasmid

    #219396

    Purpose

    Express HNF4A in mammalian cells

    Depositor

    Yoichi Furukawa

    Article

    Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Tags

    N terminal Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV_NESPylRS(AF)_hU6tRNAPyl

    Plasmid

    #223508

    Purpose

    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

    Depositor

    Edward Lemke

    Article

    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

    Inserts

    NES Pyrrolysyl-tRNA Synthetase (AF) (pylS Methanosarcina mazei)
    tRNA Pyl
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y306A; Y384F

    Promoter

    CVM, SP6 and U6

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro

    Plasmid

    #216244

    Purpose

    Express AtAFB2.F74A mutant with mCherry tag for AID2

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    AtAFB2.F74A-mCherry
    

    Use

    CRISPR, TALEN, and Unspecified ; Human safe harbo…

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    F74A mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCDNA5/avitag-apex2-v5-fkbp8

    Plasmid

    #154460

    Purpose

    expresses avitag-apex2-v5-fkbp8/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    avitag-apex2-v5-fkbp8 (FKBP8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F, T7 promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3/hArf6(Q67L)-HA

    Plasmid

    #79425

    Purpose

    Expresses C-terminally HA-tagged Arf6(Q67L) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Glutamine 67 to Leucine

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3/hArf6(T27N)-HA

    Plasmid

    #79426

    Purpose

    Expresses C-terminally HA-tagged Arf6(T27N) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Threonine 27 to Asparagine

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHes1(2.5k)-luc

    Plasmid

    #43806

    Depositor

    Ryoichiro Kageyama

    Article

    Takebayashi et al J Biol Chem. 1994 Feb 18;269(7):5150-6.

    Insert

    Hes1 Promoter (Hes1 Mouse)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains the murine Hes1 promoter

    Promoter

    Hes1 Promoter

    Available since

    March 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Frt-V5-hspB1

    Plasmid

    #63102

    Purpose

    Expression of HSPB1 (small HSP) tagged with V5 in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb1 (HSPB1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHes1(467 RBPj(-))-luc

    Plasmid

    #43805

    Depositor

    Ryoichiro Kageyama

    Article

    Nishimura et al Genomics. 1998 Apr 1;49(1):69-75.

    Insert

    Hes1 Promoter (-467 to +46) RBPj(-) (Hes1 Mouse)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains the murine Hes1 promoter (-467 to +46), …

    Promoter

    Hes1 promoter fragment (-467 to +46) (RBPj-)

    Available since

    April 5, 2013

    Availability

    Academic Institutions and Nonprofits only

41. pSAM2_mCherry_Gata4

    Plasmid

    #72690

    Purpose

    Doxycyline regulated expression of Gata4 in mammalian cells

    Depositor

    Timothy Kamp

    Article

    Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

    Insert

    Gata 4 (Gata4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-Doxycycline

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pRK5 FLAG 17xS/T->A, catalytic active lipin 1

    Plasmid

    #32007

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 (Lpin1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S106A, S150A, (S281A, T282A), S285A, S287A, S293A…

    Promoter

    CMV, SP6

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

43. pCMV_NESPylRS(WT)_hU6tRNAPyl

    Plasmid

    #223509

    Purpose

    PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

    Depositor

    Edward Lemke

    Article

    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

    Inserts

    NES Pyrrolysyl-tRNA Synthetase (pylS Methanosarcina mazei)
    tRNA Pyl
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6 and U6

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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44. plentiCRISPv2_FDX1-3

    Plasmid

    #184490

    Purpose

    Lentivirus all in one CRISPR vector targeting human FDX1 gene

    Depositor

    Peter Tsvetkov, Todd Golub

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    FDX1 (FDX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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45. plentiCRISPv2_FDX1-1

    Plasmid

    #184488

    Purpose

    Lentivirus all in one CRISPR vector targeting human FDX1 gene

    Depositor

    Peter Tsvetkov, Todd Golub

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    FDX1 (FDX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCW57-mCherry-2A-BRD4 Iso A

    Plasmid

    #137720

    Purpose

    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform

    Depositor

    Scott Floyd

    Article

    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

    Insert

    BRD4 long isoform (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. pcDNA3/hArf1(WT)-mCherry

    Plasmid

    #79419

    Purpose

    Expresses C-terminally mCherry-tagged Arf1(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf1 (ARF1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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48. pEUI(+)

    Plasmid

    #111834

    Purpose

    For mamalian expression of Ecdysone receptor-based inducible transgene expression switch.

    Depositor

    Hyunju Ro

    Article

    Lee et al Mol Ther Nucleic Acids. 2016 Sep 27;5(9)

    Insert

    GAL4-minimal VP16-EcR (EcR Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SV40, 10XUAS

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCDNA3/flag-pbira(1-g78)-sec61b

    Plasmid

    #154455

    Purpose

    expresses flag-pbira(1-g78)-sec61b/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    flag-pbira(1-g78)-sec61b (SEC61B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLVX-TetOne-Puro-PSMD4

    Plasmid

    #202666

    Purpose

    Tet-ON 3rd generation expression vector with inducible expression of PSMD4 and a puromycin selection cassette

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    proteasome 26S subunit ubiquitin receptor, non-ATPase 4 (PSMD4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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