We narrowed to 8,589 results for: Set

1. pCAG-T3-hCasD10A-pA

   Plasmid

   #51638

   Purpose

   Expresses Cas9-nickase in mammalian cells and zygotes.

   Depositor

   Wataru Fujii

   Article

   Fujii et al Biochem Biophys Res Commun. 2014 Jan 31.

   Insert

   Codon-optimized Cas9 D10A
   

   Use

   CRISPR

   Tags

   FLAG, NLS, and polyA

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

2. UMG-SP-1_pET29b

   Plasmid

   #215433

   Purpose

   Expression construct for UMG-SP-1 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   UMG-SP-1
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. UMG-SP-2_pET29b

   Plasmid

   #215434

   Purpose

   Expression construct for UMG-SP-2 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   UMG-SP-2
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFLAG-ING2

   Plasmid

   #13295

   Depositor

   Curtis Harris

   Article

   Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

   Insert

   ING2 (INGX Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

5. NylB_pET29b

   Plasmid

   #215414

   Purpose

   Expression construct for NylB gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylB
   

   Expression

   Bacterial

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pES134

   Plasmid

   #127551

   Purpose

   Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13

   Depositor

   Emmanuele Severi, Gavin Thomas

   Article

   Wu et al Microbiology (Reading). 2024 Mar;170(3).

   Insert

   F3-flanked spectinomycin resistance cassette
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pY2

   Plasmid

   #218115

   Purpose

   Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRAC_DARIC(2-3)_CD22-BBz_AAV6_Donor

   Plasmid

   #211906

   Purpose

   Vector for AAV6 donor production. Targeted CD22-BBz DARIC transgene integration to the TRAC locus via homology-directed repair.

   Depositor

   Yannick Doyon

   Article

   Levesque et al bioRxiv 2023.09.14.557485

   Insert

   DARIC-CD22-BBz
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. 5HT6-YFP-Inpp5e

   Plasmid

   #96808

   Purpose

   cilia-membrane-targeted catalytic domain of inositol phosphae 5-phosphatase, CAAX-null

   Depositor

   Takanari Inoue

   Article

   Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

   Insert

   5HT6-YFP-Inpp5e
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDM1082

    Plasmid

    #108982

    Purpose

    Knockout cassette G418

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Insert

    Knockout cassette G418
    

    Use

    Cre/Lox; Dictyostelium discoideum knockout vector

    Promoter

    coaA

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tt-NylC_pET28a

    Plasmid

    #215431

    Purpose

    Expression construct for Tt-NylC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    Tt-NylC
    

    Expression

    Bacterial

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. NmeCas9_3XNLS_pMCSG7

    Plasmid

    #120078

    Purpose

    Bacterial expression of wtNmeCas9 with 3X NLS

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    NmeCas9
    

    Expression

    Bacterial

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3XP3-WhiteA2

    Plasmid

    #165875

    Purpose

    Basic transgenesis 3xP3 white CDS physical eye marker cassette in Alpha2, lacks attB site

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Eye Marker Cassette
    

    Use

    Synthetic Biology

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSecEffector

    Plasmid

    #172891

    Purpose

    Contains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901

    Depositor

    Urartu Seker

    Article

    Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

    Inserts

    Bxb1
    TP901
    TEV Display cassette
    ProD promoter between anti aligned Bxb1 sites
    

    Expression

    Bacterial

    Promoter

    ProD, nTETO, and pBAD /AraC

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFJ496

    Plasmid

    #44488

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    500bp Mos1, unc-119, fosmid cassette
    

    Use

    Fosmid recombineering cassette

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNM3749

    Plasmid

    #154108

    Purpose

    loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Inserts

    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::his-58::FRT3
    

    Use

    Cre/Lox; Rmce

    Expression

    Worm

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330_ATP1A1_G7

    Plasmid

    #173204

    Purpose

    Expresses the ATP1A1 G7 sgRNA in combination with SpCas9 to target ATP1A1 intron 17.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G7 sgRNA + SpCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLLX8

    Plasmid

    #79838

    Purpose

    Contains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vector

    Depositor

    Cammie Lesser, Stephen Lory

    Article

    Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

    Type

    Empty backbone

    Use

    Contains yeast counter selection gene, cyh2 and b…

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJZ001

    Plasmid

    #120230

    Purpose

    Contains intB13 integrase casette for knock-in to pCAP-BAC backbone

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    intB13 integrase casette
    

    Expression

    Bacterial

    Mutation

    bla-g717a (amp resistance gene)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBALU9

    Plasmid

    #69324

    Purpose

    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd GFP intron;use C-term after STOP

    Depositor

    Oliver Hobert

    Article

    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

    Insert

    gSL2-NLS-GFPint-FgF
    

    Expression

    Bacterial

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only