We narrowed to 13,587 results for: TIM

1. pFR-mScarletI-antiFRET-P2A-jGCaMP7c

   Plasmid

   #191461

   Purpose

   Compare the intensity of the genetically encoded calcium sensor (GECI) jGCaMP7c to mScarlet-I fluorescent protein

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   jGCaMP7c
   

   Use

   Tags

   His-mScarletI-antiFRETlinker-P2A

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_H2B-STf

   Plasmid

   #181998

   Purpose

   CMV overexpression of SNAP-tag fast fusion to H2B for nuclear localization in mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   H2B-STf
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA-SCcpV

   Plasmid

   #190636

   Purpose

   Mammalian expression of genetically encoded calcium indicator SCcpV

   Depositor

   Robert Campbell

   Article

   Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

   Insert

   SCcpV
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPR2_IHF_Opt

   Plasmid

   #149395

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   IHFprox site is optimized at key positions needed…

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTrichoGate-14

   Plasmid

   #160714

   Purpose

   For C-terminal tags with Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL) bordered by BsaI restriction sites

   Depositor

   Artemio Mendoza

   Article

   Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

   Insert

   Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2-zfCSF3b

   Plasmid

   #168202

   Purpose

   Express zebrafish Csf3b, mRNA synthesis

   Depositor

   Petr Bartunek

   Article

   Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

   Insert

   Csf3b (csf3b Zebrafish)
   

   Use

   Vertebrate expression (zebrafish, chicken, xenopu…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pN1mcherry-NKD2S31A

   Plasmid

   #174332

   Purpose

   encodes human NKD2 S31A mutant cDNA with a C-terminal mCherry fusion.

   Depositor

   Sonal Srikanth

   Article

   Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

   Insert

   Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
   

   Use

   Tags

   mCherry tag

   Expression

   Mammalian

   Mutation

   changed Serine 31 to Alanine

   Promoter

   CMV

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. H2A-mTurquoise-RhoJQ79L-deltaCaax

   Plasmid

   #176110

   Purpose

   Constitutively active, nucelar localized RhoJ

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

   Insert

   RhoJ (RHOJ Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q79L

   Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.1-NKD2S31A

   Plasmid

   #174331

   Purpose

   encodes human NKD2 S31A mutant cDNA with a C-terminal MycHis Tag.

   Depositor

   Sonal Srikanth

   Article

   Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

   Insert

   Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
   

   Use

   Tags

   MycHis

   Expression

   Mammalian

   Mutation

   changed Serine 31 to Alanine

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2-zfCSF3a

    Plasmid

    #168201

    Purpose

    Express zebrafish Csf3a, mRNA synthesis

    Depositor

    Petr Bartunek

    Article

    Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

    Insert

    Csf3a (csf3a Zebrafish)
    

    Use

    Vertebrate expression (zebrafish, chicken, xenopu…

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of A27

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVMG0217_Cq-U6-2b_2xBbsI-gRNA

    Plasmid

    #169339

    Purpose

    Expression of gRNA in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0217_Cq-U6-2b_2xBbsI-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CPIJ039728

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBI-CMV1-mir374b~421-CMV2-mir1

    Plasmid

    #164674

    Purpose

    Express miR-374b~421 and miR-1-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-374b~421 (MIR374B Human)
    miR-1-1 (MIR1-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBI-CMV1-mir374b-CMV2-mir1

    Plasmid

    #164672

    Purpose

    Express miR-374b and miR-1-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-374b (MIR374B Human)
    miR-1-1 (MIR1-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBI-CMV1-mir181b-1~125-CMV2-mir1

    Plasmid

    #164671

    Purpose

    Express miR-181b-1~125a and miR-1-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-181b-1~125a (MIR181B1 Human)
    miR-1-1 (MIR1-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KG#920

    Plasmid

    #110937

    Purpose

    Expresses INS-22-Emerald in the cholinergic motor neuron DA9 for imaging Dense Core Vesicles in a single neuron in a living animal

    Depositor

    Kenneth Miller

    Article

    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

    Inserts

    mig-13 promoter
    INS-22-Emerald
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTrichoGate-15

    Plasmid

    #160715

    Purpose

    For C-terminal tags with Trichoderma optimised Synthetic 3X-FLAG peptide bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    Trichoderma optimised Synthetic 3X-FLAG peptide
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMIII-Neo-mChe

    Plasmid

    #153423

    Purpose

    Retroviral (MSCV) expression of mCherry fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    mCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. FoxFTVC+bpFOG_MEK-S216D, S220E

    Plasmid

    #107522

    Purpose

    Constitutive activation of FGF/MAPK pathway by MEK-S216D, S220E mutant

    Depositor

    Lionel Christiaen

    Article

    Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

    Insert

    MEK
    

    Use

    Tags

    Expression

    Mutation

    changed Serine 216 to Aspartic Acid and Serine 22…

    Promoter

    TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. BII-C3H2B

    Plasmid

    #133387

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET20b-YbAnbu

    Plasmid

    #129637

    Purpose

    Expression plasmid of Anbu from Y. bercovieri (UNIPROT A0A0T9RXH3 variant with V14A and G107H mutations)

    Depositor

    Matthias Bochtler

    Article

    Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

    Insert

    YbAnbu
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    V14A and G107H

    Promoter

    T7

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET20b-YbAnbuT1A

    Plasmid

    #129638

    Purpose

    Expression plasmid of Anbu from Y. bercovieri (inactive mutant; (UNIPROT A0A0T9RXH3 variant with T1A, V14A and G107H mutations)

    Depositor

    Matthias Bochtler

    Article

    Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

    Insert

    YbAnbuT1A
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    T1A, V14A and G107H

    Promoter

    T7

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTAMACHis6_PiuD

    Plasmid

    #128953

    Purpose

    expresses PiuD in E.coli

    Depositor

    James Naismith

    Article

    Luscher et al Antimicrob Agents Chemother. 2018 May 25

    Insert

    Putative outer membrane receptor for iron transport (PiuD)
    

    Use

    Tags

    6xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-105)

    Promoter

    T7

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCarCBADE

    Plasmid

    #117757

    Purpose

    Codon optimized CarCBADE enzymes from Pectobacterium carotovorum in pBbaA5k backbone

    Depositor

    Greg Bokinsky

    Article

    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

    Insert

    Codon optimised CarCBA, Protein CarD, Ferredoxin CarE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac(UV5)

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHES 946

    Plasmid

    #112644

    Purpose

    blue light inducible cryptochrome based optogenetic expression system

    Depositor

    Hana El-Samad

    Article

    Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

    Insert

    RTG3AD-GSx7-CIB1-SV40NLS (CIB1 Budding Yeast, Synthetic, Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1-PsuACR_353-EYFP

    Plasmid

    #111076

    Purpose

    Anion channelrhodopsin from Proteomonas sulcata (CCMP704) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin PsuACR_353
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pONL-C1_RhoB

    Plasmid

    #65698

    Purpose

    Expresses ONL-RhoB in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    RhoB (RHOB Human)
    

    Use

    Luciferase

    Tags

    ONL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pYNL-C1_RhoB

    Plasmid

    #65696

    Purpose

    Expresses YNL-RhoB in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    RhoB (RHOB Human)
    

    Use

    Luciferase

    Tags

    YNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pONL-N1_vinculin

    Plasmid

    #65692

    Purpose

    Expresses Vinculin-ONL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    vinculin (Vcl Mouse)
    

    Use

    Luciferase

    Tags

    ONL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

29. pYNL-N1_vinculin

    Plasmid

    #65690

    Purpose

    Expresses Vinculin-YNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    vinculin (Vcl Mouse)
    

    Use

    Luciferase

    Tags

    YNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pGEX-KG-mTmod3-150-352_domain

    Plasmid

    #70755

    Purpose

    mouse Tropomodulin3, 150-352 domain for bacterial expression

    Depositor

    Weiping Han

    Article

    Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

    Insert

    Tropomodulin3 150-352 AA fragment (Tmod3 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge