We narrowed to 2,710 results for: GEM;
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p6xUAS (GB0179)
Plasmid#68182PurposeProvides 6xUAS (upstream activating sequence) as a level 0 GoldenBraid partDepositorInsert6xUAS
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFH6_new
Plasmid#105866Purposemore efficient version of pFH6; Subcloning of any sgRNA via BbsI siteDepositorInsertU6-26p::sgRNA scaffold
UseCRISPR and Synthetic Biology; SubcloningExpressionPlantAvailable SinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSFV-Helper1
Plasmid#92073PurposeThis is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.DepositorInsertcDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
UseHelper plasmid, for run-off transcription and rna…Available SinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBES1-LacOx50-Hyg
Plasmid#210261PurposeIntegrates 50xLacO repeats upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.DepositorInsert50xLacO repeats derived from: PMID: 12864855.
Available SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-iresFUCCI
Plasmid#133371PurposePiggybac vector for constitutive expressionDepositorInsertClover-Geminin-IRES-mKO2-Cdt1
TagsFlagExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKL003
Plasmid#98919PurposeConstitutively expresses the voltage indicator PROPS under the Sigma 70 promoter in E. coli.DepositorInsertPROPS
TagsHisExpressionBacterialPromoterBBa_J23118 - Strong ConstitutiveAvailable SinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
placZα-tdMCP
Plasmid#191132PurposeExpression of tdMCP from a lacZα inducible promoterDepositorInsertdMCP_1
UseSynthetic BiologyPromoterlac-inducible promoterAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-tag
Plasmid#55180PurposeFLAG-tagDepositorInsertFLAG
UseSynthetic Biology; Bacillus biobrick boxAvailable SinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
1.3xWT HBV-Luciferase
Plasmid#61549PurposeThe 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1. Luciferase inserted within Core ORF. 2. Luciferase is regulated by EnhII - Core promoter. 3. The functional luciferase ATG is of CoreDepositorInsert1.3 times of the HBV genome with luciferase within the Core ORF
UseLuciferaseExpressionMammalianMutationSubstitution of HBV sequences with luciferase ORF…Available SinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEn-Chimera
Plasmid#61432PurposeGateway-Entry vector containing AtU6-26 promoter and sgRNA backboneDepositorInsertAtU6-26:sgRNA
UseCRISPRExpressionPlantPromoterAtU6-26Available SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFP.E227
Plasmid#138249PurposeReporter plasmid - Expresses three fluorescent reporters from three different ECF promotersDepositorInsertsmCherry
Terminator
mTagBFP
Terminator
gfp
Terminator
UseSynthetic BiologyTags6xHisExpressionBacterialPromoterP17_up1691, P20_992, and iP16_3622Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBES1-LacOx50-mCh-bla
Plasmid#210260PurposeIntegrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.DepositorInserts50xLacO repeats derived from: PMID: 12864855.
mCherry
Available SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTC217
Plasmid#70018PurposeCas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1bDepositorInsertNuclease (Cas9/sgRNA) + Donor + GVR
UseCRISPRExpressionPlantAvailable SinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFP.R270
Plasmid#138219PurposeExpresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R269
Plasmid#138181PurposeExpresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R268
Plasmid#138180PurposeExpresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
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HA-tag
Plasmid#55182PurposeHA-tagDepositorInsertHA tag
UseSynthetic Biology; Bacillus biobrick boxAvailable SinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only
