We narrowed to 14,330 results for: cas9

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N-Terminal Split Cas9 with GyrA intein

Plasmid

#58693

Purpose

Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

Depositor

Gang Bao

Article

Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

Insert

Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein

Use

AAV and CRISPR

Tags

3xFlag, GyrA Nsplit Intein, and NLS

Expression

Mammalian

Promoter

CBh

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SPdCas9-EGFP-2A-Blast

Plasmid

#71215

Purpose

dCas9 fused to EGFP

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-EGFP

Use

Lentiviral

Tags

dSpCas9-EGFP

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pRM049 His-TEV-Cas9 triNLS 2C

Plasmid

#196245

Purpose

Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

Depositor

Ross Wilson

Article

Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

Insert

Cas9

Use

CRISPR

Tags

HA, His6 (N terminal on backbone), NLS, Nucleopla…

Expression

Bacterial

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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AAV1 for Split-PE, SpCas9 nickase (PKC1001)

Plasmid

#190111

Purpose

AAV1 for expression of nickase SpCas9(H840A) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

nSpCas9

Use

AAV

Tags

bpNLS

Mutation

H840A

Promoter

EF1a

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9(D10A)-T2A-EGFP

Plasmid

#221230

Purpose

Express nCas9 nickase (D10) with GFP reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

SpCas9(D10A)-T2A-EGFP

Expression

Mammalian

Mutation

D10A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Eef1a-1955/-1>nls::Cas9::nls

Plasmid

#59987

Purpose

Eef1a (EF1alpha) promoter driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Eef1a (EF1alpha) -1955/-1

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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p415-PtrpC-Cas9-TtrpC-CYC1t

Plasmid

#68059

Purpose

Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

Depositor

Christian Hong

Article

Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

Insert

humanized Cas9

Use

CRISPR; N. crassa, fungi

Expression

Yeast

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-SlugCas9-NNG-P2A-puro

Plasmid

#214352

Purpose

Expresses SlugCas9-NNG and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugCas9-NNG and puromycin resistance genes

Expression

Mammalian

Mutation

Q782R/S888R/L906R/N984S/E1012K/K1016I

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-KRAB-dCas9-IRES-BFP

Plasmid

#85449

Purpose

Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

KRAB-dCas9-IRES-BFP

Use

CRISPR and Lentiviral

Tags

2x NLS, HA Tag, and KRAB domain

Expression

Mammalian

Promoter

TRE3G

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

Plasmid

#64512

Purpose

Expression of St1 dCas9-3XTagBFP2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XmTagBFP2

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9.EF1a-BFP.sgLMNA

Plasmid

#98971

Purpose

Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNA and spCas9

Expression

Mammalian

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-VP64-Puro

Plasmid

#99371

Purpose

3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

dCas9-VP64-T2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pYJ28-AAVS1-XLone-dCas9-p300core

Plasmid

#131071

Purpose

Donor plasmid of targeted inducible-dCAS9-P300core knockin

Depositor

Xiaojun Lian

Insert

dCas9-p300core

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-pU6-sgRNA

Plasmid

#159174

Purpose

Vector B encodes pAAV-pMecp2-dSaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of dSaCas9-sgRNA in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9(H840A)-T2A-EGFP

Plasmid

#221231

Purpose

Express nCas9 nickase (H840A) with GFP reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

SpCas9(H840A)-T2A-EGFP

Expression

Mammalian

Mutation

H840A

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgDrd2

Plasmid

#159918

Purpose

Mutagenesis of Drd2

Depositor

Larry Zweifel

Insert

Drd2 (Drd2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-enCas9(D10A)-PolI3M

Plasmid

#249061

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to PolI3M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9-PolI3M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-enCas9(H840A)-PolI5M

Plasmid

#249064

Purpose

Expresses nucleus-localized enCas9 (H840A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9(H840A)-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

Plasmid

#126706

Purpose

to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

Insert

ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)

Use

CRISPR

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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peSpCas9(1.1)-2×sgRNA (IFT88, donor)

Plasmid

#80769

Purpose

Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Insert

IFT88 gRNA#1 (IFT88 Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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