We narrowed to 4,333 results for: chm

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RBM4_2xRRM-ZNF_pGEX

Plasmid

#135154

Purpose

Recombinant expression of RBM4 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM4 (RBM4 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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tdTomato/pTREX-b

Plasmid

#68709

Purpose

Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.

Depositor

Roberto Docampo

Article

Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

Insert

tdTomato

Use

Expression of tdtomato red fluorescence protein i…

Available Since

Oct. 8, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pDGE667

Plasmid

#153232

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE479

Plasmid

#153270

Purpose

M4E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pHsSR beta deltaN Flag

Plasmid

#13731

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (SRPRB Human)

Tags

6xHis and FLAG

Expression

Bacterial

Mutation

expresses residues 59-271 of Homo sapiens SRβ wit…

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Poly(A)

Plasmid

#65524

Purpose

Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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XE122 XDsh delta DIX-GFP-CS2+

Plasmid

#16787

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1999 Jul 26;146(2):427-37.

Insert

Xdsh-delta DIX (dvl2.L Frog)

Use

Xenopus expression

Tags

gfp

Mutation

deletion of aa 1-159

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only

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pDGE463

Plasmid

#153234

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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SRSF9_2xRRM_pGEX

Plasmid

#135120

Purpose

Recombinant expression of SRSF9 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SRSF9 (SRSF9 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDGE670

Plasmid

#153236

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE331

Plasmid

#153240

Purpose

M1E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-tetR-T2A-iRFP

Plasmid

#176028

Purpose

construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720

Depositor

Irmela Jeremias

Article

Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

Inserts

tetR
iRFP

Use

Lentiviral

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-MGT1-d2EGFP_CMV-mCherry

Plasmid

#213763

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

MGT#1-d2EGFP

Use

Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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SRSF8_RRM_pGEX

Plasmid

#135119

Purpose

Recombinant expression of SRSF8 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SRSF8 (SRSF8 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221 Cas9-POLD3

Plasmid

#183197

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-POLD3

Use

CRISPR; Gateway entry clone

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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RBM23_2xRRM_pGEX

Plasmid

#135118

Purpose

Recombinant expression of RBM23 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM23 (RBM23 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE652

Plasmid

#153230

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-6p-hDcp2

Plasmid

#72216

Purpose

bacterial expression of GST-tagged human Dcp2

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Tags

GST

Expression

Bacterial

Mutation

F146Y and E198K (please see depositor's comm…

Promoter

tac

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
NUPL2_ZNF_pGEX

Plasmid

#135135

Purpose

Recombinant expression of NUPL2 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

NUPL2 (NUPL2 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag-hDcp2

Plasmid

#72213

Purpose

mammalian expression of Flag-tagged human Dcp2

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Q170R (please see depositor's comment below)

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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