We narrowed to 23,430 results for: Spr

1. MsCLYBL_sgRNA6

   Plasmid

   #111295

   Purpose

   CRISPR KO murine CLYBL

   Depositor

   Vamsi Mootha

   Article

   Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

   Insert

   sgRNA6 against murine CLYBL
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBLlSh2

   Plasmid

   #170981

   Purpose

   Expression of ShCAST under control of lacI Plac promoter/repressor pair.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Inserts

   lacI
   ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLlac01

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR1055

   Plasmid

   #71823

   Purpose

   Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLS

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLH-stsgRNA1.1

   Plasmid

   #64116

   Purpose

   Vector for expression of St1 sgRNA1.1 in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   St1 sgRNA1.1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAct-dCBEevoAPOBEC1

   Plasmid

   #195514

   Purpose

   Ubiquitous expression of dCBEevoAPOBEC1 in Drosophila

   Depositor

   Michael Boutros

   Article

   Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

   Insert

   dCBE-evoAPOBEC1
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   act5C

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTX172

   Plasmid

   #89259

   Purpose

   Express STU (Single Transcriptional Unit) Cas9 with Maize Ubiquitin1 promoter in plants

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTX176

   Plasmid

   #89260

   Purpose

   Express estrodial-inducible STU (Single Transcriptional Unit) Cas9 in plants

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-GBP-dCas9-mRFP

   Plasmid

   #102933

   Purpose

   Expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)

   Depositor

   Sebastian Bultmann

   Article

   Anton et al Nucleus. 2017 May 4;8(3):279-286. doi: 1

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   GBP (GFP binding protein) and mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pEF:iCas9

   Plasmid

   #138267

   Purpose

   Expression of mTN3-GGS6-dCas9 (iCas9) in human.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   mTN3-GGS6-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Cas9-D10A, H840A

   Promoter

   Ef1a

   Available since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZmU3-gRNA

    Plasmid

    #53061

    Purpose

    Transcription of guide RNA (gRNA) in maize cells

    Depositor

    Caixia Gao

    Article

    Liang et al J Genet Genomics. 2014 Feb 20;41(2):63-8

    Insert

    zmU3 promoter - gRNA
    

    Use

    CRISPR; Plant expression

    Tags

    Expression

    Mutation

    Promoter

    maize U3

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJA59

    Plasmid

    #59932

    Purpose

    gRNA for cleavage at unc-109(n499) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    unc-109(n499) gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTX168

    Plasmid

    #89257

    Purpose

    Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330A_FokI-1x4

    Plasmid

    #63604

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCfB8622

    Plasmid

    #126912

    Purpose

    Plasmid containing gRNA expression cassette targeting ADE2 in Saccharomyces cerevisiae

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    ADE2 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKEW321-MbCas12a-RFP-GFP-array

    Plasmid

    #115661

    Purpose

    Encodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    GFP and RFP array for MbCas12
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJEC578

    Plasmid

    #174366

    Purpose

    dCas9 for modular CRISPRa. SYNZIP-dCas9. Expresses dCas9 with a SYNZIP18 domain fused to the N terminus.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Insert

    dCas9 fused to SYNZIP18
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23150

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti

    Plasmid

    #138318

    Purpose

    Lenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFP

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    SauCas9 GFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330_HR_Prnp_3

    Plasmid

    #78621

    Purpose

    pX330 vector encoding SpCas9 and a chimeric guide RNA targeting Prnp coding sequence

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

    Insert

    pX330_HR_Prnp_3
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330A_dCas9-1x6

    Plasmid

    #63600

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D1361Y

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ159

    Plasmid

    #69308

    Purpose

    Gateway entry vector with hSpCas9D10A

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    hSpCas9D10A (Human codon optimized)
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mutation

    D10A mutation; NLS fusion

    Promoter

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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