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Showing: 441 - 480 of 1308 results

  13. 4A3.PBAD-repL(WT).J23115-gfp

    Plasmid
    #197872
    Purpose
    Arabinose induced expression of wildtype repL gene, which triggers DNA replication in cis and in trans, weak level of constitutive GFP expression
    Depositor
    Baojun Wang
    Article
    Huan et al Front Microbiol. 2023 Feb 9;14:1095671.
    Inserts
    part kilA/repL
    gfp
    Use
    Tags
    Expression
    Bacterial
    Mutation
    contains only the 24 bp of 3'-end of kilA ge…
    Promoter
    Bba_J23115 and PBAD
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197872-plasmid-map-sequence-id-395257

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  17. pBAD-6xHis-AviTag-ALFAtag-eGFP

    Plasmid
    #195854
    Purpose
    Bacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.
    Depositor
    John Ngo
    Article
    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103
    Insert
    6xHis-AviTag-ALFAtag-eGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195854-plasmid-map-sequence-id-383965

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  20. pBEST-p15a-pBAD-UTR1-TetR-T500

    Plasmid
    #45787
    Depositor
    Richard Murray
    Insert
    Tetracycline repressor
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    July 16, 2013
    Availability
    Academic Institutions and Nonprofits only
    45787-plasmid-map-sequence-id-64258

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  23. N295A (EcN), N229A (EcQ) MetNIQ in pBAD

    Plasmid
    #118581
    Purpose
    N295A Mutation of MetN in Methionine ABC Transporter and N229A Mutation in Methionine Binding Protein (MetQ) for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (metN E. coli)
    MetI (metI E. coli)
    MetQ (metQ E. coli)
    Use
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Asparagine (Asn) 229 to Alanine (Ala) and…
    Promoter
    None and araBAD Promotoer
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118581-plasmid-map-sequence-id-228549

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  24. N295A (EcN), Y160A (EcI) MetNIQ in pBAD

    Plasmid
    #118260
    Purpose
    N295A Mutation of MetN and Y160A in MetI in Methionine ABC Transporter for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (Y160A) (metI E. coli)
    MetQ (metQ E. coli)
    Use
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118260-plasmid-map-sequence-id-228496

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  26. N295A (EcN), M163A (EcI) MetNIQ in pBAD

    Plasmid
    #118261
    Purpose
    N295A Mutation of MetN and M163A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (M163A) (metI E. coli)
    MetQ (metQ E. coli)
    Use
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118261-plasmid-map-sequence-id-228528

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  27. N295A (EcN), M107A (EcI) MetNIQ in pBAD

    Plasmid
    #118259
    Purpose
    N295A Mutation of MetN and M107A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (M107A) (metI E. coli)
    MetQ (metQ E. coli)
    Use
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118259-plasmid-map-sequence-id-228500

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  28. N295A (EcN), F103A (EcI) MetNIQ in pBAD

    Plasmid
    #118258
    Purpose
    N295A Mutation of MetN and F103A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (F103A) (metI E. coli)
    MetQ (metQ E. coli)
    Use
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118258-plasmid-map-sequence-id-228498

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  30. T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB

    Plasmid
    #110547
    Purpose
    CRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.
    Depositor
    Ichiro Matsumura
    Insert
    dCas9
    Use
    Tags
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pBAD
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    110547-plasmid-map-sequence-id-210556

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  32. PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid
    #225649
    Purpose
    tetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance
    Depositor
    Andre Sanches Ribeiro
    Article
    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/
    Insert
    tetR/tetA, araBAD and LacO3O1 in tandem conformation
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    tetA, araBAD and LacO3O1 promoters
    Available since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    225649-plasmid-map-sequence-id-448716

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  34. pKPY115

    Plasmid
    #72669
    Purpose
    Encodes Thr251Gly-EcPheRS Under Arabinose-Inducible (PBAD) Control
    Depositor
    David Tirrell
    Insert
    Thr251Gly-EcPheRS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Thr251Gly in Alpha Subunit
    Promoter
    PBAD
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    72669-plasmid-map-sequence-id-133221

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  35. pOPARA1

    Plasmid
    #200416
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200416-plasmid-map-sequence-id-394143

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  36. pOPARA2

    Plasmid
    #200417
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (spectinomycin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200417-plasmid-map-sequence-id-394144

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  37. pWT018a

    Plasmid
    #107885
    Purpose
    R3 plasmid. Contains PBAD-EGFP and is part of the CAMERA system for rewritable multi-event analog recording in bacteria
    Depositor
    David Liu
    Article
    Tang et al Science. 2018 Feb 15. pii: science.aap89
    Insert
    PBAD-EGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107885-plasmid-map-sequence-id-207292

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  38. pWT004a

    Plasmid
    #107883
    Purpose
    R1 plasmid. Contains PBAD-EGFP and is part of the CAMERA system for rewritable multi-event analog recording in bacteria
    Depositor
    David Liu
    Article
    Tang et al Science. 2018 Feb 15. pii: science.aap89
    Insert
    PBAD-EGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107883-plasmid-map-sequence-id-207299

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Showing: 441 - 480 of 1308 results