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Showing: 551 - 600 of 2950 results

  8. pCLAP

    Plasmid
    #200786
    Purpose
    Cloning template for N-terminal CLIPf tagging and C-terminal SNAPf tagging of constructs for mammalian expression. Also used for the mammalian expression of CLIPf-SNAPf-HisTag-FLAG fusion protein.
    Depositor
    Alison Twelvetrees
    Article
    Smith et al bioRxiv 2024.12.20.629623
    Type
    Empty backbone
    Use
    Tags
    CLIPf and SNAPf-6xHis-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    200786-plasmid-map-sequence-id-435692

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  9. SH4-FRB-EBFP2

    Plasmid
    #222418
    Purpose
    To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells
    Depositor
    Stephen Royle
    Article
    Downie et al bioRxiv 2024.05.31.596797
    Insert
    SH4
    Use
    Tags
    FRB-HA-EBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    222418-plasmid-map-sequence-id-439139

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  14. GFP-LiMETER-v1

    Plasmid
    #113933
    Purpose
    Express an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFP
    Depositor
    Yubin Zhou
    Article
    Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.
    Insert
    LOV2 and Rit polybasic domain (PB)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    113933-plasmid-map-sequence-id-225305

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  17. pGal-Trans-Myc-HA-GFP11-KDEL

    Plasmid
    #177724
    Purpose
    ER protein collagen beta (1-O) galactosyltransferase with a C-terminal Myc tag, an HA tag and the eleventh β-strand of GFP
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)
    Use
    Tags
    GFP11, HA, KDEL, and Myc
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177724-plasmid-map-sequence-id-352616

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  18. pAAV-hSyn-GluA2-scFv-mNeon-KDEL

    Plasmid
    #230057
    Purpose
    AAV plasmid that expresses a a single chain variable fragment (scFv) for anti- GluA2 with a C-terminal mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA2 single chain variable fragment (scFv)
    Use
    AAV
    Tags
    ER retrieval motif (KDEL) and mNeon
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230057-plasmid-map-sequence-id-457175

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  20. pAAV-hSyn-GluA1-scFv-mNeon-KDEL

    Plasmid
    #230056
    Purpose
    AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 tagged with mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA1 single chain variable fragment (scFv)
    Use
    AAV
    Tags
    ER retrieval motif (KDEL) and mNeon
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230056-plasmid-map-sequence-id-457171

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  38. pREXC3GH

    Plasmid
    #47077
    Purpose
    FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags
    Depositor
    Eric Geertsma, Raimund Dutzler
    Article
    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.
    Type
    Empty backbone
    Use
    Lactococcus lactis expression
    Tags
    3C protease site, GFP, and His
    Expression
    Mutation
    Promoter
    NIS (nisin)
    Available since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    47077-plasmid-map-sequence-id-67103

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  42. pcDXC3GMS

    Plasmid
    #49031
    Purpose
    FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, EGFP, Myc tag and streptavidin binding peptide
    Depositor
    Eric Geertsma, Raimund Dutzler
    Article
    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.
    Type
    Empty backbone
    Use
    Tags
    3C protease site (PreScission site), EGFP, MYC, a…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    49031-plasmid-map-sequence-id-71989

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Showing: 551 - 600 of 2950 results