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We narrowed to 601 results for: bira

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Showing: 221 - 240 of 601 results

  1. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid
    #79889
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Expression
    Bacterial
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79889-plasmid-map-sequence-id-150065

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  4. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-M566T

    Plasmid
    #218832
    Purpose
    Expresses N-terminal BirA*-tagged human TLK1 variant 5 mutant M566T for BioID experiments.
    Depositor
    Travis Stracker
    Insert
    TLK1 M566T (TLK1 Human)
    Tags
    FLAG-BirA*
    Expression
    Mammalian
    Mutation
    M566T
    Promoter
    CMV
    Available Since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218832-plasmid-map-sequence-id-434422

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  5. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-Q479E

    Plasmid
    #218833
    Purpose
    Expresses N-terminal BirA*-tagged human TLK1 variant 5 mutant Q479E for BioID experiments.
    Depositor
    Travis Stracker
    Insert
    TLK1 Q479E (TLK1 Human)
    Tags
    FLAG-BirA*
    Expression
    Mammalian
    Mutation
    Q479E
    Promoter
    CMV
    Available Since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218833-plasmid-map-sequence-id-434421

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  9. phage - ubc - nls - 2xmcp - egfp - cMycBirA*

    Plasmid
    #131133
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Insert
    NLS-HA-2xMCP-EGFP-cMycBirA*
    Use
    Lentiviral
    Tags
    NLS, HA, cMyc (N-terminal of BirA*)
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available Since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131133-plasmid-map-sequence-id-255501

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  11. Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

    Plasmid
    #208048
    Purpose
    Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    BirAopt
    Use
    Lentiviral
    Mutation
    BirAopt is human-codon-optimized BirA (PMID 15707…
    Promoter
    EFS
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208048-plasmid-map-sequence-id-410202

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  14. piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)

    Plasmid
    #209086
    Purpose
    Ngn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expression
    Depositor
    Vikram Khurana
    Article
    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.
    Inserts
    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    Tags
    AviTag
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    TRE4G and hSyn
    Available Since
    Dec. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    209086-plasmid-map-sequence-id-417159

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  16. Lenti-EFS-BirAopt-GSQ-RNF4ca-P2A-BLAST

    Plasmid
    #208047
    Purpose
    Enables constitutive expression of N-terminal BirAopt-fused RNF4ca (catalytically inactive RING mutant); to perform bioE3; selection with blasticidin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    RNF4 (RNF4 Human)
    Use
    Lentiviral
    Tags
    BirAopt
    Mutation
    Cys>Ala mutations in conserved RING domain
    Promoter
    EFS
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208047-plasmid-map-sequence-id-410205

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  18. pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT

    Plasmid
    #89890
    Purpose
    BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d
    Insert
    HA-BirA-2A-citrine
    Use
    Unspecified
    Tags
    Citrine
    Available Since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89890-plasmid-map-sequence-id-181309

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Showing: 221 - 240 of 601 results