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Plasmid#183277PurposeAll-in-One CRISPRko system with a guide RNA that targets CSF1R geneDepositorInsertCSF1R
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPU-huTRIMmiR30-TRIM5
Plasmid#132938PurposeAll-in-one huTRIM5 rescue-TRIM5 shRNA knockdownDepositorInserthuman TRIM5
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterSFFVAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_NEMF
Plasmid#127126DepositorInsertgRNA NEMF (NEMF Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4-miR30-TRIM5
Plasmid#132932PurposeLentivector for TRIM5 knockdown and puromycin resistanceDepositorInsertTRIM5 shRNA
UseLentiviral and RNAiTagsExpressionMutationPromoterSFFVAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-Otx2shRNA1
Plasmid#73981PurposeOtx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.DepositorInsertshRNA that targets the mouse Otx2 gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_TEAD3_1
Plasmid#86337PurposeEncodes gRNA for 3' target of human TEAD3DepositorInsertgRNA against TEAD3 (TEAD3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
gRNA[bTub].1026C
Plasmid#112691Purposeexpress gRNA targeting bTub under dU6-3 promoterDepositorInsertU6.3-gRNA[bTub]
UseCRISPRTagsExpressionMutationPromoterdU6-3Available sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Dgcr8_2
Plasmid#73526PurposeExpresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8DepositorInsertDGCR8
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
shCDK6(2)
Plasmid#73553PurposeshRNA(2) against CDK6DepositorInsertshRNA against CDK6
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
gRNA_SRR124.5_tagBFP
Plasmid#163753PurposegRNA expression vector containing the tagBFP fluorescent marker to target the region upstream of the SRR124 SOX2 enhancer in human cells.DepositorInsertgRNA_SRR124.5
UseCRISPRTagstagBFPExpressionMammalianMutationPromoterU6Available sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC3
Plasmid#191858PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330A-Blimp1-L1-#1
Plasmid#171503Purposedeletion of a genomic locus in Blimp1(Prdm1) geneDepositorInsertBlimp1 (Prdm1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
E42_control_NGFR
Plasmid#189800PurposeRetroviral delivery of control guide RNADepositorInsertLuciferase gRNA
UseRetroviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_HLA_DRB
Plasmid#164992PurposeExpression of gRNA targeting HLA-DRB locus, including DRB1*01:02:01DepositorInsertgRNA against HLA-DRB1*01:02:01
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_TYMS
Plasmid#183326PurposeAll-in-One CRISPRko system with a guide RNA that targets TYMS geneDepositorInsertTYMS
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_KDR
Plasmid#183305PurposeAll-in-One CRISPRko system with a guide RNA that targets KDR geneDepositorInsertKDR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
1046E=dgRNAs[traB,bTub]
Plasmid#149425PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes.DepositorInsertU6.3-gRNAs[TraB, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterU6.3Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNAftsI
Plasmid#149649Purposeall-in-one CRISPRi vector for targeting B. burgdorferi ftsIDepositorInsertdCas9, lacI, sgRNAftsI
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_P0526-sgRNAftsI
Plasmid#149650Purposeall-in-one CRISPRi vector for targeting B. burgdorferi ftsIDepositorInsertdCas9, lacI, sgRNAftsI
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, P0526Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNAftsI
Plasmid#149563PurposeftsI gRNA expression in B. burgdorferiDepositorInsertsgRNAftsI
UseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only