Addgene

Showing: 2381 - 2400 of 2899 results

Results per page:

Sort results by:

FSW-HRP-V5-MDGA1

Plasmid

#82541

Purpose

Lentiviral vector for expressing HRP-MDGA1 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

MDGA1 (MDGA1 Human)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Mutation

Promoter

Human Synapsin

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

Plasmid

#173865

Purpose

Mammalian expression of mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

Use

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CRY-tTA (B702)

Plasmid

#92032

Purpose

Expresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Use

Tags

tTA2 (TetR fused to VP64)

Expression

Mammalian

Mutation

NLS on CRY2 is mutated

Promoter

CMV

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGY466

Plasmid

#166661

Purpose

For light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeast

Depositor

Gaël Yvert

Article

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Cre: E340A, D341A

Promoter

MET17

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
DF-UAS-spa-GCaMP6f

Plasmid

#67568

Purpose

Zebrafish expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6f

Use

Zebrafish transgenesis (see :a spinal opsin contr…

Tags

HIS-tag

Expression

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only
dmBACCS2VL-IRES-dOrai-IRES-GFP

Plasmid

#72897

Purpose

Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2VL-IRES-dOrai-IRES-GFP

Use

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CNC3G (CASANOVA-C3 with glycine linkers)

Plasmid

#137192

Purpose

Expresses CNC3G (CASANOVA-C3 with glycine linkers), a blue light dependent NmeCas9 inhibitory protein

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2021 Mar 18;49(5):e29

Insert

CNC3G (CASANOVA-C3 with glycine linkers)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Cry2 mCherry RGS4(del 1-33) in pcDNA3.1

Plasmid

#64207

Purpose

mCherry fused between Cry2 and RGS4(del 1-33) to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 (CRY2 Mustard Weed)
RGS4(del 1-33) (RGS4 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pEF1a-CRY2PHR-SID4X

Plasmid

#63663

Purpose

AAV expression of CRYS2PHR-SID4X

Depositor

Dong Kong

Article

Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

Insert

SID4X-CYR2PHR

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
FSW-HRP-V5-MDGA2

Plasmid

#82542

Purpose

Lentiviral vector for expressing HRP-MDGA2 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

MDGA2 (Mdga2 Rat)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Mutation

Promoter

Human Synapsin

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IP-EGFP-LOV2-OMP25

Plasmid

#233346

Purpose

Stable expression of EGFP-LOV2(N538E)-OMP25 in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

Depositor

Noboru Mizushima

Article

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

LOV2 domain of phototropin1
Omp25 (Synj2bp Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

N-terminus is deleted and N538E

Promoter

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB26 (punc-17::GtACR2::mCerulean::βHK::Chrimson)

Plasmid

#214889

Purpose

Expresses the optogenetic tool BiPOLES in cholinergic neurons of C. elegans.

Depositor

Alexander Gottschalk

Article

Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

Insert

GtACR2_mCerulean_bHK_Chrimson

Use

Tags

mCerulean

Expression

Worm

Mutation

Promoter

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB27 (pmyo-3::GtACR2::mCerulean::βHK::Chrimson)

Plasmid

#214890

Purpose

Expresses the optogenetic tool BiPOLES in BWMs of C. elegans.

Depositor

Alexander Gottschalk

Article

Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

Insert

GtACR2_mCerulean_bHK_Chrimson

Use

Tags

mCerulean

Expression

Worm

Mutation

Promoter

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB31 (punc-47::GtACR2::mCerulean::bHK::Chrimson)

Plasmid

#214891

Purpose

Expresses the optogenetic tool BiPOLES in GABAergic neurons of C. elegans.

Depositor

Alexander Gottschalk

Article

Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

Insert

GtACR2_mCerulean_bHK_Chrimson

Use

Tags

mCerulean

Expression

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS2156

Plasmid

#158099

Purpose

High Copy number plasmid containing SYN1 insert

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

SYN1 (SYN1 Human)

Use

Tags

Expression

Mutation

Promoter

SYN1

Available since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pX552-EF1a-DIO Chronos-eGFP(with gRNA scaffold)

Plasmid

#199582

Purpose

Expresses Chronos-eGFP in a Cre-dependent manner

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Chronos eGFP

Use

AAV, CRISPR, and Cre/Lox

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a intron A

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX552 EF1a DIO Chronos GFP_Vgat gRNA

Plasmid

#215277

Purpose

Expresses Vgat gRNA in a Cre dependent Chronos GFP vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Vgat gRNA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX552 EF1a DIO Chronos GFP_VgatTTT gRNA

Plasmid

#215278

Purpose

Expresses Vgat Control gRNA in a Cre dependent Chronos GFP vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Vgat control gRNA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
OptoProfilin.S138E

Plasmid

#208287

Purpose

Expresses Cry2(1-531)-mCherry-Profilin.S138E

Depositor

Robert Hughes

Article

Mann et al Chembiochem. 2024 May 2;25(9):e202400007

Insert

Cry2 - mCherry - Profilin.S138E (Cry2, PRF1 Mouse, Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Ser 138 (sometimes numbered 137) mutated to Glu

Promoter

CMV

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
OptoProfilin.S138A

Plasmid

#208288

Purpose

Expresses Cry2(1-531)-mCherry-Profilin.S138A

Depositor

Robert Hughes

Article

Mann et al Chembiochem. 2024 May 2;25(9):e202400007

Insert

Cry2 - mCherry - Profilin (Cry2, PRF1 Mouse, Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Ser 138 (sometimes numbered 137) mutated to Ala

Promoter

CMV

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge