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Plasmid#180318Purposemammalian expression of human SEPT2 fused to monomeric superfolder GFPDepositorInsertSEPTIN2 (SEPTIN2 Human)
UseTagsmsfGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSSRB274_pAC8-eGFP-hsCRBN
Plasmid#218791PurposeInsect cell expression vector for FLAG-TEV-eGFP-Prescission-hsCRBNDepositorInsertProtein cereblon (CRBN Human)
UseTagsFLAG-TEV-eGFP-PrescissionExpressionInsectMutationeGFP fused to N-terminusPromoterpolyhedrinAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-EFNB2 (D62Q-Q130L-V167L)
Plasmid#200977PurposeMammalian expression plasmid for myc-tagged EFNB2 (D62Q-Q130L-V167L specificity mutant)DepositorInsertEFNB2 (EFNB2 Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationD62Q-Q130L-V167L (increase specificity towards he…PromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
CA-RIT-NFAT1 (PGK-Puro)
Plasmid#63214PurposeConstitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interactio. Contains Puromycin selection markerDepositorInsertCA-RIT-NFAT1 (Nfatc2 Mouse)
UseRetroviralTagsHAExpressionMammalianMutationAmino acids 1-3 removed; CA: PASSGSSASF mutated t…PromoterAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV_GFP10-14-SEPT7
Plasmid#180341Purposemammalian expression of human SEPT7 fused to GFP10DepositorInsertSEPTIN7 (SEPTIN7 Human)
UseTagsGFP10ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTT5-hSDC1-CS1/CS2
Plasmid#52370Purposeexpresses human Syndecan-1 point mutant at S184A and S194A in HEK293-EBNA1 (293E) suspension culture.DepositorInsertSDC1 (SDC1 Human)
UseTagsExpressionMammalianMutationR95Q, S184A and S194A (according to numbering in …PromoterCMVAvailable sinceDec. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEN6-Ubc5 Sc
Plasmid#83234PurposepHis-par2-UbHs/Ubc5Sc/ Uba1TaDepositorUseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 HA D122A pcDNA3
Plasmid#206120Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1DepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationD122APromoterCMVAvailable sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA E1658A (EIVA) pcDNA3
Plasmid#206114Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE1658APromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 EIVA (E1707A) pcDNA3
Plasmid#206125Purposeexpression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation bindingDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationE1707APromoterCMVAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 HA EIA (E320A) pcDNA3
Plasmid#206124Purposeexpression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation bindingDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationE320APromoterCMVAvailable sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV_mApple-SEPT7 Gmut2
Plasmid#180317Purposemammalian expression of human SEPT7 Gmut2 fused to mAppleDepositorInsertSEPTIN7 (SEPTIN7 Human)
UseTagsmAppleExpressionMammalianMutationSEPT7 H279DPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1-14-GFP10
Plasmid#180343Purposemammalian expression of human SEPT9_i1 fused to GFP10DepositorInsertSEPTIN9_v1 (SEPTIN9 Human)
UseTagsGFP10ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ289-RVR
Plasmid#138127PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a with D513R, K519V and N523R mutations, without promoterDepositorInsertErCas12a RVR variant
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationD513R, K519V and N523RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
eIF3f- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192242Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3fDepositorInserteIF3f-knockin-homology arm-mAID-mCherry2-Hygro (EIF3F Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ284-RVR
Plasmid#138117PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V and N573R mutations, without promoterDepositorInsertMb2Cas12a RVR variant
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationN563R, K569V and N573RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTT5-hSDC1-CS1
Plasmid#52365Purposeexpresses human Syndecan-1 point mutant at S184A in HEK293-EBNA1 (293E) suspension culture.DepositorInsertSDC1 (SDC1 Human)
UseTagsExpressionMammalianMutationR95Q and S184A (according to numbering in Fig. 2A…PromoterCMVAvailable sinceDec. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQC MCS IRES G418
Plasmid#110344Purposeγ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Geneticin selection.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-PRMT3(E338Q)
Plasmid#164696PurposeExpression of PRMT3(E338Q) with an N-terminal FLAG tagDepositorInsertPRMT3 (PRMT3 Human)
UseTagsFLAGExpressionMammalianMutationE338QPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTT5-hSDC1-S15A
Plasmid#52363Purposeexpresses human Syndecan-1 serine 15 mutated to alanine (numbering excludes signal peptide) in HEK293-EBNA1 (293E) suspension culture.DepositorInsertSDC1 (SDC1 Human)
UseTagsExpressionMammalianMutationS15A and R95Q (according to numbering in Fig. 2A …PromoterCMVAvailable sinceDec. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mEtfdh
Plasmid#232314PurposeExpression of mouse mutant EtfdhDepositorInsertEtfdh (Etfdh Mouse)
UseLentiviralTagsExpressionMutationPAM Sequence Mutations: (G36C, T39C, C42T, C72T, …PromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pSDC803_IKZF1_mutant
Plasmid#224113PurposeInsect cell expression vector for IKZF1 mutant for CRBN cryoEM pre-blottingDepositorInsertIKZF1 (IKZF1 Human)
UseTagsFlag-TEVExpressionInsectMutationResidues 140-196, Q146A and G151NPromoterpolyhedrinAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AGTR1-DuET
Plasmid#213183PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertAGTR1 (AGTR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Mts
Plasmid#195065PurposeExpresses FLAG-tagged Drosophila mts proteinDepositorInsertmicrotubule star (mts Fly)
UseTags3xFLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Pp2A-29B
Plasmid#195066PurposeExpresses FLAG-tagged Drosophila Pp2A-29B proteinDepositorInsertProtein phosphatase 2A at 29B (Pp2A-29B Fly)
UseTags3xFLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIVA (E1658A) pCAGGS
Plasmid#206082Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE1658APromoterCMV/B-actinAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EIA (E314A) pCAGGS
Plasmid#206081Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain I which reduces trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE314APromoterCMV/B-actinAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only